A Study of Nucleic Acid Synthesis in Ascites Tumor Cells by Two-Emulsion Autoradiography

Baserga, Renato

doi:10.1083/jcb.12.3.633

Cited by 57 publications

(6 citation statements)

References 17 publications

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“…The binding of histone polypeptides was assayed by selective dissociation with the ionic detergent, sodium deoxycholate. Since histones appear to repress genome transcription (although perhaps in a nonspecific fashion) the tight binding of these proteins to DNA during mitosis is consistent with the restriction of mitotic RNA synthesis in vitro (Stein & Farber, 1972;Johnson & Holland, 1965) and in vivo (Taylor, 1960;Baserga, 1962;Prescott & Bender, 1962). However, the similarity of S-phase and mitotic histones makes it unlikely that they alone can account for cell-cycle-stage-specific variations in their binding to DNA.…”

Section: Discussionmentioning

confidence: 81%

Non-histone chromosomal proteins. Evidence for their role in mediating the binding of histones to deoxyribonucleic acid during the cell cycle

Stein

Hunter

Lavie

1974

Biochemical Journal

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By selective dissociation of histones with the ionic detergent sodium deoxycholate, we have demonstrated that these basic chromosomal polypeptides, which are effective inhibitors of transcription, are more tenaciously bound to DNA in mitotic than in S-phase chromatin. Evidence is presented which suggests that cell-cycle-stage-specific non-histone chromosomal proteins can account for such variations in the association of histones with DNA. When chromatin is reconstituted with DNA and histones are pooled from S-phase and mitotic cells and either S-phase or mitotic non-histone chromosomal proteins, a preferential extraction of histones with sodium deoxycholate from chromatin reconstituted with S-phase rather than mitotic non-histone chromosomal proteins is observed. In contrast, the extractability of histones with sodium deoxycholate from nucleohistone complexes reconstituted with DNA pooled from S-phase and mitotic cells and either S-phase or mitotic histones is identical. Since non-histone chromosomal proteins rather than histones are responsible for the differences in chromatin template activity during S-phase and mitosis, we propose that non-histone chromosomal proteins may modify gene expression during the cell cycle by mediating the binding of histones to DNA.

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Section: Discussionmentioning

confidence: 81%

Non-histone chromosomal proteins. Evidence for their role in mediating the binding of histones to deoxyribonucleic acid during the cell cycle

Stein

Hunter

Lavie

1974

Biochemical Journal

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“…The most striking fact is that metaphase-arrested cells undergo apoptosis but interphase cells do not, so it makes sense to consider which structural and biochemical differences in mitotic cells might facilitate the induction of apoptosis. For example, decreased RNA and protein synthesis at mitosis [35]- [38] may hinder the cell's ability to mount a heat shock response. Lack of a nuclear envelope during mitosis may allow certain factors to reach the chromatin that would not do so during interphase.…”

Section: Discussionmentioning

confidence: 99%

Moderate Hyperthermia Induces Apoptosis in Metaphase-Arrested Cells But Not in Interphase Hela Cells

Paulson¹,

Kresch²,

Mesner³

2016

ABC

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Metaphase-arrest agents and hyperthermia are both known to be capable of inducing apoptosis, and they have been used, separately, in cancer treatments. Here, we have examined whether the two treatments together may have a synergistic effect. We find that when H-HeLa cells are arrested in metaphase with spindle poisons (nocodazole or paclitaxel) and then subjected to mild heat treatment (41.5˚C), they exhibit morphological changes typical of apoptosis within three hours. Moreover, those changes are blocked by the pan-caspase inhibitor zVAD-fmk, indicating apoptosis, and activated Procaspase 3 is detected by immunoblotting and by staining with the fluorescein-labelled caspase inhibitor FAM-VAD-fmk. Interphase cells treated in the same way do not undergo apoptosis, even with spindle poisons present. Induction of apoptosis is more rapid when the cells have been arrested longer in metaphase, suggesting that accumulation or depletion of some cellular component(s) during metaphase-arrest may make them more susceptible to hyperthermia. Further work is in progress to test whether other cell lines exhibit the same behavior and to learn more about the mechanism. The phenomenon is of interest because it may provide clues to how hyperthermia induces cell death and may yield novel therapeutic approaches to block or stimulate apoptosis.

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“…G2 marks the gap between the end of S phase and the start of mitosis. Here, the cell synthesizes materials needed for mitosis, such as RNA and proteins (3).…”

Section: The Cell Cycle In Normal Cellsmentioning

confidence: 99%

Cell-cycle Checkpoints and Aneuploidy on the Path to Cancer

Wenzel

Singh

2018

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A Study of Nucleic Acid Synthesis in Ascites Tumor Cells by Two-Emulsion Autoradiography

Cited by 57 publications

References 17 publications

Non-histone chromosomal proteins. Evidence for their role in mediating the binding of histones to deoxyribonucleic acid during the cell cycle

Non-histone chromosomal proteins. Evidence for their role in mediating the binding of histones to deoxyribonucleic acid during the cell cycle

Moderate Hyperthermia Induces Apoptosis in Metaphase-Arrested Cells But Not in Interphase Hela Cells

Cell-cycle Checkpoints and Aneuploidy on the Path to Cancer

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