A study of low power laser on the regenerative process of Girardia tigrina (Girard,1850) (Turbellaria; Tricladida; Dugesiidae)

Lopes, Kar.; Velho, Nádia Maria Rodrigues de Campos; Munin, Egberto

doi:10.1590/s1519-69842009000200013

Cited by 6 publications

(5 citation statements)

References 17 publications

(17 reference statements)

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“…The last two species L. friderici and S. brevipinna were the most 13 C depleted fishes and they have similar feeding habits. Both fishes are iliophagous and feed on detritus, benthic filamentous algae and invertebrates associated to bottom substrates [52], [53].…”

Section: Resultsmentioning

confidence: 99%

Methane Carbon Supports Aquatic Food Webs to the Fish Level

et al. 2012

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Large amounts of the greenhouse gas methane (CH4) are produced by anaerobic mineralization of organic matter in lakes. In spite of extensive freshwater CH4 emissions, most of the CH4 is typically oxidized by methane oxidizing bacteria (MOB) before it can reach the lake surface and be emitted to the atmosphere. In turn, it has been shown that the CH4-derived biomass of MOB can provide the energy and carbon for zooplankton and macroinvertebrates. In this study, we demonstrate the presence of specific fatty acids synthesized by MOB in fish tissues having low carbon stable isotope ratios. Fish species, zooplankton, macroinvertebrates and the water hyacinth Eichhornia crassipes were collected from a shallow lake in Brazil and analyzed for fatty acids (FA) and carbon stable isotope ratios (δ13C). The fatty acids 16∶1ω8c, 16∶1ω8t, 16∶1ω6c, 16∶1ω5t, 18∶1ω8c and 18∶1ω8t were used as signature for MOB. The δ13C ratios varied from −27.7‰ to −42.0‰ and the contribution of MOB FA ranged from 0.05% to 0.84% of total FA. Organisms with higher total content of MOB FAs presented lower δ13C values (i.e. they were more depleted in 13C), while organisms with lower content of MOB signature FAs showed higher δ13C values. An UPGMA cluster analysis was carried out to distinguish grouping of organisms in relation to their MOB FA contents. This combination of stable isotope and fatty acid tracers provides new evidence that assimilation of methane-derived carbon can be an important carbon source for the whole aquatic food web, up to the fish level.

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Section: Resultsmentioning

confidence: 99%

Methane Carbon Supports Aquatic Food Webs to the Fish Level

et al. 2012

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“…As regards OM healing, LLLT is known to increase fibroblast proliferation , favoring the collagen synthesis and angiogenesis ; reducing COX‐2, TNF‐α and pro‐inflammatory cytokines IL‐6 and IL‐1β ; promoting differentiation of anti‐inflammatory cytokines IL‐2, IL‐4, IL‐8 and IL‐10 ; and acting in the NFkB pathway . In addition to analgesia, LLLT can enhance peripheral endogenous opioid production and decrease serum prostaglandin E2 .…”

Section: Introductionmentioning

confidence: 99%

Comparative study among three different phototherapy protocols to treat chemotherapy‐induced oral mucositis in hamsters

Campos

Cruz

Pereira

et al. 2016

Journal of Biophotonics

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In this study, clinical, biochemical and histological analysis were used to compare different phototherapies, including LED, low and high-power laser (HPL) for the treatment of chemotherapy (CT)-induced oral mucositis (OM). One-hundred-fifty hamsters were divided into five groups: C: control; CH: CT/OM induction; L: CT/OM induction and treatment with LED (635 nm, 1.2 J), HL: CT/OM induction and treatment with HPL (808 nm, 10 J), LL: CT/OM induction and treatment with low-level laser therapy (LLLT) (660 nm, 1.2 J). OM was induced by scratches performed on check pouch mucosa after two injections of 5-Fluorouracil. The experiment lasted 10 days and OM was analyzed by specific clinical scales on days 5, 7 and 10. The animals were euthanized and the cheek pouch mucosa removed for biochemical (TNF-α concentration) and histological (light microscopy) analysis. After statistical analysis, the authors' results showed LED and LLLT therapies were efficient treatments for OM, decreasing TNF-α concentration on day 7 (p < 0.05) and completely healing the mucosa on day 10. HPL showed no interference in final healing of OM. According to the methodology used and the results obtained in the present study, LLLT and LED therapies were the best choices to decrease the severity of OM, accelerating tissue repair and decreasing the inflammatory process. Clinical evaluation of OM in Groups CH, LL, L and HL and their respective arrangement of phototherapy treatments at different time intervals (5, 7 and 10 days).

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“…This species is notable for presenting steroids, triterpenoids, flavonoids, tannins and saponins, which are chemical compounds that have pharmacological properties (Hnatyszyn et al, 2007). Moreover, several studies have shown that the use of low-power laser has beneficial therapeutic results on the cicatrization in different tissues (Lopes et al, 2009;Moraes et al, 2012). The main medical applications of LLLT are in reducing pain and inflammation, augmenting tissue repair and promoting regeneration of different tissues and nerves, and in preventing tissue damage in situations where it is likely to occur (Byrnes et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Wound-Healing potential of Sebastiania hispida (Mart.) Pax (Euphorbiaceae) ointment compared to low power laser in rats

et al. 2016

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Impaired wound healing represents a serious complication in some pathologies and the use of plant extracts has proved to improve tissue repair. The objectives of this study were to evaluate the healing potential of the ointment of Sebastiana hispida compared with Aluminum-Gallium Indium-Phosphide Laser (InGaAlP) in surgically induced wounds in rats and to perform the phytochemical analysis. The phytochemical analysis was performed in the classic way and also by HPLC. A controlled study was developed with 80 rats (200-250 g) in which a linear excision was performed in the dorsal region after shaving, measuring 2 cm × 1 cm (epidermis and dermis) exposing the muscle fascia. The rats were randomly divided into four groups of twenty animals each. The experimental groups (n = 5) were G1 (Saline); G2 (crude methanol plant extract 2% + Carbopol Gel 98%); G3 (crude methanol plant extract 2% + lanolin/vaseline) and G4 (laser). The incision healing processes were monitored during 3, 7, 14 and until 21 days after excision. The histologic parameters evaluated were Collagen fiber types, microscopic examination and neovascularization. There was a significant increase in the deposition of collagen fibers, as evidenced by a better organized epithelial tissue, keratinized and showing greater proliferation of new blood vessels in the inflammatory phase in the group treated with both the extract and laser. The results were correlated to the phenolic derivatives found after qualitative and quantitative analysis. These compounds were considered responsible for the healing process. The topical treatment with S. hispida leaves, in the two different formulations, was more effective than the application of the laser (Ingan ALP) 660 nm in the model used.Keywords: laser therapy, tissue repair, inflammatory cells, medicinal plants. Potencial cicatrizante da pomada de Sebastiania hispida (Mart.) Pax (Euphorbiaceae) comparado ao laser de baixa potência ResumoA cicatrização deficiente representa uma complicação grave em algumas patologias e o uso de extratos de plantas tem demonstrado melhora no processo de reparação tecidual. O objetivo deste estudo foi avaliar o potencial cicatrizante da pomada de Sebastiania hispida comparado ao Laser Indio Phosphide-Gallium-Aluminum (InGaAlP) em feridas cutâneas induzidas cirurgicamente em ratos e realizar analise fitoquímica. A análise fitoquímica foi via clássica e por CLAE. O experimento foi desenvolvido com 80 ratos (200-250 g), divididos aleatoriamente em quatro grupos (n = 5): G1 (salina); G2 (extrato bruto metanólico 2% + Carbopol Gel 98%); G3 (extrato bruto metanólico 2% + lanolina/ vaselina) e G4 (laser). Após a tricotomia da região dorsal realizou uma excisão linear, medindo 2 cm × 1 cm (epiderme e derme), expondo a fáscia muscular. Em sequência os tratamentos e o monitoramento do processo de cicatrização das feridas cirúrgicas ocorreu nos tempos de: 3, 7, 14 e até 21 dias após a excisão. Os parâmetros histológicos avaliadosBraz.

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A study of low power laser on the regenerative process of Girardia tigrina (Girard,1850) (Turbellaria; Tricladida; Dugesiidae)

Cited by 6 publications

References 17 publications

Methane Carbon Supports Aquatic Food Webs to the Fish Level

Methane Carbon Supports Aquatic Food Webs to the Fish Level

Comparative study among three different phototherapy protocols to treat chemotherapy‐induced oral mucositis in hamsters

Wound-Healing potential of Sebastiania hispida (Mart.) Pax (Euphorbiaceae) ointment compared to low power laser in rats

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