A study of low-energy ion induced radiolysis of thiol-containing amino acid cysteine in the solid and aqueous solution states

Ke, Zhixin; Huang, Qing; Bingrong, Dang; Lü, Yue; Yuan, Hang; Zhang, Shuqing; Zhang, Yu

doi:10.1016/j.nimb.2010.06.017

Cited by 17 publications

(13 citation statements)

References 39 publications

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“…[49] Similarly, the sulfhydryl group in cysteine is also susceptible to the reactive species produced by discharge plasma, as previously reported by us. [30] In the present work, Raman spectroscopy was used to investigate the change of the state of sulfur of GSH as a result of discharge plasma treatment. As seen from Figure 1, the most striking differences of GSH Raman spectra before and after discharge are both in the ÀSH and ÀSÀSÀ stretching vibration region: the Raman peak of ÀSÀSÀ stretching vibration at 508 cm À1 appeared after discharge treatment, concomitantly with a decrease in the intensity of the ÀSH stretching vibration band at 2 567 cm…”

Section: Formation Of Gssg and Quantification Of [Gssg]/[gsh]mentioning

confidence: 99%

“…[30] It is also of great importance from the biological point of view since its production within cells is likely toxic. After collecting the gas emitted from the gas outlet, the reagent presented amethyst with maximum absorption at 670 nm, while the control showed no color change and no absorption in the same region.…”

Section: Formation Of Hydrogen Sulfidementioning

confidence: 99%

“…Previously, our group have reported the damage of amino acids (phenylalanine and cysteine) and bases by non-thermal discharge plasma at the gas-solution interface. [29][30][31] In this paper, the damage of reduced glutathione (g-L-glutamyl-L-cysteinyl-glycine, GSH) under the same conditions was investigated. GSH is considered as the most abundant low molecular weight thiol-containing antioxidant in vivo, [32] with concentration in the range of 1-10 mM in the cytosol of cells.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Damage of Glutathione by Glow Discharge Plasma at the Gas–Solution Interface through Raman Spectroscopy

Zhang

Huang

2012

Plasma Processes & Polymers

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Although non‐thermal discharge plasma is more and more applied in biological field, the molecular mechanisms of plasma acting on biomolecules are still unclear, which are indispensable for understanding the plasma‐induced biological effects. In this work, discharge plasma at the gas–solution interface was employed to irradiate on reduced glutathione (GSH), the most abundant low molecular weight thiol‐containing antioxidant in cells. Through Raman spectroscopy, both the irreversible damage and reversible conversion of GSH to oxidized glutathione (GSSG) were monitored in a non‐destructive fashion, so that the reaction kinetic processes through multi‐pathways could be evaluated quickly and quantitatively. Based on the experimental data, the reaction mechanism was discussed. magnified image

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Section: Formation Of Gssg and Quantification Of [Gssg]/[gsh]mentioning

confidence: 99%

Section: Formation Of Hydrogen Sulfidementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of Damage of Glutathione by Glow Discharge Plasma at the Gas–Solution Interface through Raman Spectroscopy

Zhang

Huang

2012

Plasma Processes & Polymers

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“…It was observed that OH radical dependent abstraction of a hydrogen atom from the α-carbon of amino acids, aliphatic side chains of hydrophobic amino acid residues of protein and from the protein polypeptide backbone seem to be the possible initial sites of attack 31 32 36 . There are some studies of the plasma action on amino acids by different research groups 37 38 39 40 . They observed the disulfide linkage formation and hydroxylation of amino acids after the plasma treatment.…”

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confidence: 99%

Influence of reactive species on the modification of biomolecules generated from the soft plasma

Attri

Kumar

Park

et al. 2015

Sci Rep

108

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Plasma medicine is an upcoming research area that has attracted the scientists to explore more deeply the utility of plasma. So, apart from the treating biomaterials and tissues with plasma, we have studied the effect of soft plasma with different feeding gases such as Air, N2 and Ar on modification of biomolecules. Hence, in this work we have used the soft plasma on biomolecules such as proteins ((Hemoglobin (Hb) and Myoglobin (Mb)), calf thymus DNA and amino acids. The structural changes or structural modification of proteins and DNA have been studied using circular dichroism (CD), fluorescence spectroscopy, protein oxidation test, gel electrophoresis, UV-vis spectroscopy, dynamic light scattering (DLS) and 1D NMR, while Liquid Chromatograph/Capillary Electrophoresis-Mass Spectrometer (LC/CE-MS) based on qualitative and quantitative bio-analysis have been used to study the modification of amino acids. Further, the thermal analysis of the protein has been studied with differential scanning calorimetry (DSC) and CD. Additionally, we have performed docking studies of H2O2 with Hb and Mb, which reveals that H2O2 molecules preferably attack the amino acids near heme group. We have also shown that N2 gas plasma has strong deformation action on biomolecules and compared to other gases plasma.

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“…Comparable results were obtained when treating GapDH protein, which was also inactivated more rapidly in a liquid compared to a dried environment . Ke et al also demonstrated that cysteine is affected more quickly when treated under liquid conditions, whereas treatment under dried conditions was less efficient at damaging the amino acid. Grzegorzewski et al investigated the influence of both atmospheric‐pressure (Ar‐fed jet) and low‐pressure (RF glow‐discharge used with oxygen) plasmas on dried 1,4‐benzopyrone derivates.…”

Section: Resultsmentioning

confidence: 97%

A combined low‐pressure hydrogen peroxide evaporation plus hydrogen plasma treatment method for sterilization − Part 2: An intercomparison study of different biological systems

Lackmann

Fiebrandt

Raguse

et al. 2016

Plasma Processes & Polymers

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Low‐pressure plasmas are a promising alternative to modern sterilization processes. As plasma is a surface process, multilayered stacks of spores are a crucial challenge to overcome. Here, a combined process of condensed hydrogen peroxide and hydrogen plasma is analyzed for its efficacy against various spore concentrations showing a clear increase in efficacy using a combined process compared to the two steps used separately. Besides spores, protein contaminations are a major issue in clinics and the combined process is investigated for protein removal efficiency using the well‐established BSA model. Furthermore, RNase A serves as a difficult‐to‐inactivate protein model to investigate protein inactivation efficiency. Finally, inactivation mechanisms of RNase A with a special focus on sulfur‐based modifications are investigated using Raman spectroscopy.

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A study of low-energy ion induced radiolysis of thiol-containing amino acid cysteine in the solid and aqueous solution states

Cited by 17 publications

References 39 publications

Assessment of Damage of Glutathione by Glow Discharge Plasma at the Gas–Solution Interface through Raman Spectroscopy

Assessment of Damage of Glutathione by Glow Discharge Plasma at the Gas–Solution Interface through Raman Spectroscopy

Influence of reactive species on the modification of biomolecules generated from the soft plasma

A combined low‐pressure hydrogen peroxide evaporation plus hydrogen plasma treatment method for sterilization − Part 2: An intercomparison study of different biological systems

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