A Study of Isolation and Identification of Campylobacter Species, Listeria  Monocytogenes E. Coli O157:h7 and Salmonella Species From Raw Chicken Carcasses and Beef Meat by Multiplex Polymer Chain Reaction in Baghdad City.

Khalil., ZenaK

doi:10.21474/ijar01/5134

Cited by 2 publications

(3 citation statements)

References 12 publications

(12 reference statements)

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“…The results of this study indicated that the highest rate of infection with E.coli was found in the age group(1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) months by (48%), followed by the age group of (12-24) months by (28%), and the age group (36-48) by (14%). While the lowest rate of infection with E.coli was within the age group (24-36) months by(6%), and the age group (48-60) months by (4%), as shown in Table(2).…”

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confidence: 72%

“…E.coli has many virulence factors that enable the bacteria to cause infection and disease in human; the most important of these are intestinal diseases represented by watery and bloody diarrhea which called diarrheagenic E. coli (DEC), urinary tract infection (UTIs) which called uropathogenic E. coli (UPEC), sepsis, meningitis and conjunctivitis (Khalil., 2016;Al-Kalifawi, 2013) Interestingly, some strains of E.coli have the ability to overcome the immune system and cause disease due to their possession of a set of encoded genes located at specific sites known as pathogen city islands (PAIs) (Al-Ibrahim& Hadi,2015; Hassan and Mahmood,2019 ).PAIs are more present in pathogenic strains of E.coli than in non-pathogenic strains, which code for a number of virulence factors such as adhesions, toxins, siderophores, capsules, lipopolysaccharides (LPS),Biofilm (Ghafil,2018), enzymes and type III secretory systems, TypeIII secretion systems, haemolysin, alkaline protease, and antibiotic resistance (Ramírez & Eckhard, 2022; Abdul-Ghaffar and Abu-Risha,2017 ).…”

Section: Introductionmentioning

confidence: 99%

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Detection the Phylogenetic groups of E. coli that isolated from diarrheal in children under five years and study their relationship of common serotypes in Baghdad hospitals

2023

JPTCP

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The study included 50isolates of E.coli isolatesthat were obtained from (120) stool samples collected from children with diarrhea of both sexes who visited hospitals in Baghdad. E.coli serotypes were diagnosed according to serotyping method for different age groups of children under five years. The results were represented by (7) (14%) isolates of E.coli belonging to Anticoli 1 group, (10) (20%) isolates belonging to Anticoli 11 group, (5) (23%) isolates belonging to Anticoli 111 group and (22) (44%) isolates which included (2/22) (9%)belongs to serotypes O111, O78 by (2/22) (9%), and for each of the serotypes O25, O142, and O119 are (3/22) and by 14%, O55 (5/22) ) at 23% and O44 (4/22) by 18%. As for the rest of the isolates, no results were given, which is represented by (28) (56%) unknown isolates which were not classified for any of the serotypes. Moreover, the results showed that the highest percentage of diarrhea was in males at (56%), while in females it was(44%); in addition the highest percentage of infection with E. coli in males and females was within the age group (1-12) months by (48%), followed by the age group of (12-24) months by (28%), and then the age group (36-48) by 14%, while the lowest rate of E.coli infection in males and females found in the age group (24-36) months by (6%) followed by the age group (48-60) monthsby(4%).The results of the phylogenetic groups detected several groups includinggroup A (2/50, 4%), B1 (0/50, 0%), B2 (1/50, 2%), C (12/50, 24%), D (6/50,12%), F (3/50,6%), Clade1 (0%) and E (26/50, 52%) which is the predominant group.

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confidence: 72%

Section: Introductionmentioning

confidence: 99%

Detection the Phylogenetic groups of E. coli that isolated from diarrheal in children under five years and study their relationship of common serotypes in Baghdad hospitals

2023

JPTCP

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“…PCR was performed in 50µL final volume solution for each sample consisting of 25 µL OneTaq® Hot Start Quick-Load 2X Master Mix with Standard Buffer (NEB), 0.4 µM primers (for S . Typhimurium/ L. monocytogenes ) as described by Anju, Latha, Sunil et al, 2014 [20] and Khalil 2017 [21] respectively, 5 µL of the target bacterial DNA and UltraPure™ DNase/RNase-Free Water (Invitrogen) up to a final volume of 50 µL.…”

Section: Methodsmentioning

confidence: 99%

A comparison of different DNA extraction methods and molecular techniques for the detection and identification of foodborne pathogens

2021

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<abstract> <p>Foodborne infections continue to plague Europe. Food safety monitoring is in crisis as the existing techniques for detecting pathogens do not keep up with the global rising of food production and consumption. Thus, the development of innovative techniques for detecting and identifying pathogenic bacteria has become critical. The aim of the present study was firstly to develop an innovative simple and low cost method of extracting bacterial DNA from contaminated food and water samples with <italic>Salmonella enteric</italic> subsp. <italic>enteric</italic> serovar Typhimurium and <italic>Listeria monocytogenes</italic> and its comparison with two commercial DNA extraction kits (Qiagen, Macherey-Nagel). Finally, pathogens' detection using two molecular techniques (PCR-electrophoresis, LAMP), in order to evaluate the best combination of DNA extraction and identification based on their sensitivity, cost, rapidity and simplicity. Considering the above criteria, among them, best was proved an in-house bacterial DNA extraction method, based on the chloroform-isoamyl alcohol protocol, with certain modifications. This technique showed statistically similar results in terms of sensitivity, compared to the commercial kits, while at the same time maintained high rapidity and much lower cost. Lastly, between the molecular techniques, LAMP was found more promising considering its simplicity, high rapidity and sensitivity. Conclusively, the in-house DNA extraction method along with the LAMP technique, was proven to be the best among the presented combinations.</p> </abstract>

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A Study of Isolation and Identification of Campylobacter Species, Listeria Monocytogenes E. Coli O157:h7 and Salmonella Species From Raw Chicken Carcasses and Beef Meat by Multiplex Polymer Chain Reaction in Baghdad City.

Cited by 2 publications

References 12 publications

Detection the Phylogenetic groups of E. coli that isolated from diarrheal in children under five years and study their relationship of common serotypes in Baghdad hospitals

Detection the Phylogenetic groups of E. coli that isolated from diarrheal in children under five years and study their relationship of common serotypes in Baghdad hospitals

A comparison of different DNA extraction methods and molecular techniques for the detection and identification of foodborne pathogens

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