Regional differences in water properties and responses to stressors are key to understanding ecosystem vulnerabilities in coastal regions globally. Our study focuses on Canada's British Columbia coastal waters, from Queen Charlotte Strait to the Strait of Georgia. This area is bisected by shallow, narrow, tidally mixed channels with some of the strongest tidal currents in the world. We examine differences between regions on either side of this constriction, focusing on physical water properties and responses to three potential physical and biogeochemical stressors: nutrient levels, marine heatwaves, and ocean acidification. Our results quantify a spatially abrupt and temporally persistent lateral gradient in temperature, salinity, and density co-located with a previously documented strong mixing zone. The distributions of density on either side of this front remain largely distinct throughout the tidal cycle, for all seasons, and for over 70 individual years for which data are available. Additionally, nutrient concentrations and molar ratios north of the front are statistically distinct from those to the south, and regions north of the front responded more strongly to the arrival of the marine heatwave known as "The Blob". Lastly, regional differences in sensitivity to ocean acidification are identified, with high 2 pCO E values at the surface in the Discovery Islands making that area very sensitive to ocean acidification. These results demonstrate the degree to which dramatic water property differences in adjacent coastal regions can persist over a wide range of time scales, with important connections to their responses to environmental stressors and implications for present and future ecosystem vulnerability.Plain Language Summary Regional differences in coastal waters are key to understanding marine ecosystem vulnerabilities. Our study focuses on British Columbia Canada, from Queen Charlotte Strait to the Strait of Georgia. This area is bisected by shallow, narrow channels mixed by some of the strongest tidal currents globally. We examine how water properties vary on either side of this constriction, and further how each region responds to stressors such as nutrient levels, marine heatwaves, and ocean acidification. Our results show that water properties such as temperature, salinity, and density differ between the two halves of the study area, and persist throughout the tidal cycle and year-round, for over 70 individual years. Additionally, the levels of nutrients and their relative concentrations differ, and regions north of the front experienced more extreme temperatures following the arrival of the marine heatwave known as "The Blob". Lastly, regions on either side of the front are found to have different sensitivities to ocean acidification, with the highest sensitivity in the Discovery Islands. These results indicate that adjacent coastal regions can have dramatic water property differences that persist over a wide range of time scales and may respond differently to environmental stressors, with importan...