A study of genetic variability of human parainfluenza virus type 1 in Croatia, 2011–2014

Košutić-Gulija, Tanja; Slović, Anamarija; Ljubin‐Sternak, Sunčanica; Mlinarić‐Galinović, Gordana; Forčić, Dubravko

doi:10.1099/jmm.0.000297

Cited by 8 publications

(9 citation statements)

References 41 publications

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“…In several countries multiple lineages of HPIV-3 were observed within the same country in a single season or year showing that multiple lineages can co-circulate. Co-circulation of multiple HPIV-3 lineages was also observed previously in a study in Croatia [46]. The co-circulation of multiple clades is not uncommon for viruses and has been observed in other paramyxoviruses like RSV subtypes A and B and human metapneumovirus with subtypes A1, A2, B1, and B2 [50,51].…”

Section: Resultssupporting

confidence: 70%

“…Since these few sequences represented all the sequences available for these two countries, it is unclear if other clades co-circulate with clade 2 in these regions. Previous studies have found these same clades co-circulating in Croatia, Japan, USA, and Vietnam [36,40,41,46,47]. Combined, the results of these studies suggest that there may have only been two main clades of HPIV-1 circulating globally during the period included in this analysis.…”

Section: Resultssupporting

confidence: 56%

“…Cluster B was much smaller than cluster C with sequences mostly from Australia collected in 2007, one sequence from South Africa in 2007, and one recent 2016 sequence from USA. Previous studies with the HN gene have also identified a small number of sequences from cluster B in Canada and Japan [46,48,49]. Noteworthy, there were no cluster C representative sequences from Australia.…”

Section: Resultsmentioning

confidence: 95%

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Sequencing and analysis of globally obtained human parainfluenza viruses 1 and 3 genomes

Bose

Shrivastava

et al. 2019

PLoS ONE

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Human Parainfluenza viruses (HPIV) type 1 and 3 are important causes of respiratory tract infections in young children globally. HPIV infections do not confer complete protective immunity so reinfections occur throughout life. Since no effective vaccine is available for the two virus subtypes, comprehensive understanding of HPIV-1 and HPIV-3 genetic and epidemic features is important for diagnosis, prevention, and treatment of HPIV-1 and HPIV-3 infections. Relatively few whole genome sequences are available for both HPIV-1 and HPIV-3 viruses, so our study sought to provide whole genome sequences from multiple countries to further the understanding of the global diversity of HPIV at a whole-genome level. We collected HPIV-1 and HPIV-3 samples and isolates from Argentina, Australia, France, Mexico, South Africa, Switzerland, and USA from the years 2003–2011 and sequenced the genomes of 40 HPIV-1 and 75 HPIV-3 viruses with Sanger and next-generation sequencing with the Ion Torrent, Illumina, and 454 platforms. Phylogenetic analysis showed that the HPIV-1 genome is evolving at an estimated rate of 4.97 × 10 −4 mutations/site/year (95% highest posterior density 4.55 × 10 −4 to 5.38 × 10 −4 ) and the HPIV-3 genome is evolving at a similar rate (3.59 × 10 −4 mutations/site/year, 95% highest posterior density 3.26 × 10 −4 to 3.94 × 10 −4 ). There were multiple genetically distinct lineages of both HPIV-1 and 3 circulating on a global scale. Further surveillance and whole-genome sequencing are greatly needed to better understand the spatial dynamics of these important respiratory viruses in humans.

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Section: Resultssupporting

confidence: 70%

Section: Resultssupporting

confidence: 56%

Section: Resultsmentioning

confidence: 95%

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Sequencing and analysis of globally obtained human parainfluenza viruses 1 and 3 genomes

Bose

Shrivastava

et al. 2019

PLoS ONE

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“…For human parainfluenza virus 1 (KBPV-VR-44), the 1,992 bases of the HN gene were obtained by RT-nested PCR following the previously published method 28 . The obtained genomic RNA sequences and RT-PCR-positive amplicons were further analysed with related sequences in GenBank using MAFFT 29 and MEGA version 6 30 .…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of novel bat paramyxovirus B16-40 potentially belonging to the proposed genus Shaanvirus

Noh

Jeong

Yoon

et al. 2018

Sci Rep

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The bat paramyxovirus B16-40 was first isolated in Korea in this study. Using the isolated virus, we could obtain not only genomic information, but also several biological characteristics of the virus. In the phylogenetic analysis, the virus was found to belong to the recently proposed genus Shaanvirus. Through sequence analyses and in vitro testing, the isolated virus was also found to have haemagglutinin-neuraminidase (HN) protein as one of the structural proteins. When mouse antiserum was generated against the isolated virus and tested, it was cross-reactive to human parainfluenza virus 1 in an indirect immunofluorescence assay but could not cross-neutralize human parainfluenza virus 1. In addition, the bat paramyxovirus B16-40 was not infectious in the mouse model. Collectively, this study provided basic information on further classification of the bat paramyxovirus B16-40 and related viruses in the proposed genus Shaanvirus.

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“…In a recent phylogenetic analysis, the HPIV3 cluster C/subcluster C3 (genetic lineage C3a) was found to be the most frequent circulating HPIV3 strain among Croatian children with respiratory diseases [8]. However, the significance of other types, especially HPIV serotype 1, in epidemic years should be emphasized as well [3, 9, 10].…”

Section: Introductionmentioning

confidence: 99%

An Outbreak of Human Parainfluenza Virus 3 (Phylogenetic Subcluster C5) Infection among Adults at a Residential Care Facility for the Disabled in Croatia, 2018

Čivljak¹,

Košutić-Gulija

Slović

et al. 2019

Intervirology

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Introduction: Although highly pertinent for children, outbreaks of human parainfluenza virus (HPIV) may cause up to 15% of all respiratory illnesses in adults and predispose them to serious adverse outcomes, with HPIV serotype 3 (HPIV3) being the most common. This study represents the first report of an HPIV3 outbreak among adults at a long-term health-care facility in Croatia. Methods: A retrospective study was conducted to investigate an outbreak of acute respiratory infection (ARI) at a single residential care facility for the disabled in Croatia. Demographic, epidemiological, and clinical data were collected for all residents, while hospitalized patients were appraised in detail by laboratory/radio-logical methods. Multiplex PCR for respiratory viruses and sequencing was performed. Partial HPIV3 HN 581 nt sequences were aligned with HPIV3 sequences from the Gen-Bank database to conduct a phylogenetic analysis, where different bioinformatic approaches were employed. Results: In late June 2018, 5 of the 10 units at the facility were affected by the outbreak. Among the 106 residents, 23 (21.7%) developed ARI, and 6 (26.1%) of them were hospitalized. HPIV3 was identified in 18 (73%) of the residents and 5 (83%) of the hospitalized individuals. Isolated HPIV3 strains were classified within the phylogenetic subcluster C5 but grouped on 2 separate branches of the phylogenetic tree. During the entire outbreak period, none of the institution's employees reported symptoms of ARI. Conclusions: Our study has shown that this health care-associated outbreak of HPIV3 infection could have been linked to multiple importation events. Preventive measures in curbing such incidents should be enforced vigorously.

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A study of genetic variability of human parainfluenza virus type 1 in Croatia, 2011–2014

Cited by 8 publications

References 41 publications

Sequencing and analysis of globally obtained human parainfluenza viruses 1 and 3 genomes

Sequencing and analysis of globally obtained human parainfluenza viruses 1 and 3 genomes

Isolation and characterization of novel bat paramyxovirus B16-40 potentially belonging to the proposed genus Shaanvirus

An Outbreak of Human Parainfluenza Virus 3 (Phylogenetic Subcluster C5) Infection among Adults at a Residential Care Facility for the Disabled in Croatia, 2018

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