A study of genes involved in adipocyte differentiation

Zhu, Shunming; Cheng, Gong; Zhu, Huolan; Guan, Gongchang

doi:10.1515/jpem-2014-0002

Cited by 14 publications

(7 citation statements)

References 7 publications

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“…The effect of ANGPTL4 on adipose tissue accretion would later be reinforced by the synthesis of other cytokines such as VEGF, whose concentration we found to increase in the last trimester of pregnancy. These results are in accordance with other studies reporting that ANGPTL4 expression is significantly upregulated in obesity, in the early 18 and the later phases of adipocyte differentiation 19 , 37 , and also in adipose tissue cells from subjects with obesity 21 , 36 .…”

Section: Discussionsupporting

confidence: 93%

Angiopoietin-like protein 4 (ANGPTL4) is related to gestational weight gain in pregnant women with obesity

Ortega-Senovilla

Poppel

Desoyé

et al. 2018

Sci Rep

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Angiopoietin-like protein 4 (ANGPTL4) is a circulating protein involved in the regulation of adipose tissue metabolism. However, its role in obesity and pregnancy is unknown. To evaluate the relationship between gestational weight gain (GWG) and circulating concentrations of ANGPTL4 in pregnant women with overweight and obesity, weight gain and fasting maternal blood samples of thirty-one pregnant women was drawn at 15, 24 and 32 weeks of gestation. ANGPTL4 concentrations continuously rose throughout gestation, whereas VEGF and leptin did not show the same trend. NEFA and glycerol concentrations remained stable during pregnancy. In contrast, total concentrations of saturated, monounsaturated and n-6 fatty acids, but not n-3 fatty acids, increased with pregnancy. In multiple regression analysis, the increase in plasma ANGPTL4 and decrease in linoleic acid concentrations were the most significant predictors of GWG, although only ANGPTL4 was significantly associated with the weight gain from early pregnancy (area under the ROC curve was 0.80 p < 0.01(95% CI 0.61–0.99)). In conclusion, in pregnant women with overweight and obesity, an increase in plasma ANGPTL4 concentrations throughout pregnancy is positively associated with GWG and could be used as an early marker of increased susceptibility to excess gestational weight gain.

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Section: Discussionsupporting

confidence: 93%

Angiopoietin-like protein 4 (ANGPTL4) is related to gestational weight gain in pregnant women with obesity

Ortega-Senovilla

Poppel

Desoyé

et al. 2018

Sci Rep

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“…The gene of LYRM1 is highly expressed in omental adipose tissue of obese individuals and is an obesity-related gene involved in adipocyte differentiation and growth [ 7 , 68 , 69 ]. The overexpression of LYRM1 in mature 3T3-L1 adipocytes resulted in reduced insulin-stimulated uptake of glucose and impaired mitochondrial function [ 70 ].…”

Section: The Diverse Roles Of the Lyr Motif Proteins In Mitochondrmentioning

confidence: 99%

Eukaryotic LYR Proteins Interact with Mitochondrial Protein Complexes

Angerer

2015

Biology

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In eukaryotic cells, mitochondria host ancient essential bioenergetic and biosynthetic pathways. LYR (leucine/tyrosine/arginine) motif proteins (LYRMs) of the Complex1_LYR-like superfamily interact with protein complexes of bacterial origin. Many LYR proteins function as extra subunits (LYRM3 and LYRM6) or novel assembly factors (LYRM7, LYRM8, ACN9 and FMC1) of the oxidative phosphorylation (OXPHOS) core complexes. Structural insights into complex I accessory subunits LYRM6 and LYRM3 have been provided by analyses of EM and X-ray structures of complex I from bovine and the yeast Yarrowia lipolytica, respectively. Combined structural and biochemical studies revealed that LYRM6 resides at the matrix arm close to the ubiquinone reduction site. For LYRM3, a position at the distal proton-pumping membrane arm facing the matrix space is suggested. Both LYRMs are supposed to anchor an acyl-carrier protein (ACPM) independently to complex I. The function of this duplicated protein interaction of ACPM with respiratory complex I is still unknown. Analysis of protein-protein interaction screens, genetic analyses and predicted multi-domain LYRMs offer further clues on an interaction network and adaptor-like function of LYR proteins in mitochondria.

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“…The encoded protein is known to be induced by peroxisome proliferation activators and functions as a hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. Its expression is strongly induced during adipocyte as well as sebocyte differentiation [19–21], supporting it’s up-regulation during meibocyte differentiation. Secondly, PLIN2 encodes a protein that is associated with surface membrane of lipid droplet, and has been shown to be involved in the development and maintenance of adipose tissue.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis after PPARγ activation in human meibomian gland epithelial cells (hMGEC)

2019

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Purpose PPARγ plays a critical role in the maturation of immortalized human meibomian gland epithelial cells (hMGEC). To further understand the molecular changes associated with meibocyte differentiation, we analyzed transcriptome profiles from hMGEC after PPARγ activation. Methods Three sets of cultivated hMGEC with or without exposure to PPARγ agonist, rosiglitazone were used for RNA-seq analysis. RNA was isolated and processed to generate 6 libraries. The libraries were then sequenced and mapped to the human reference genome, and the expression results were gathered as reads per length of transcript in kilobases per million mapped reads (RPKM) values. Differential gene expression analyses were performed using DESeq2 and NOISeq. Gene ontology enrichment analysis (GOEA) was performed on gene sets that were upregulated or downregulated after rosiglitazone treatment. Five genes were selected for validation and differential expression was confirmed using quantitative PCR. The Differential expression of CK5 was evaluated using Western blotting. Results Expression data indicated that about 58,000 genes are expressed in hMGEC. DESeq2 and NOISeq indicated that 296 and 3436 genes were upregulated and 258 and 3592 genes were down regulated after rosiglitazone treatment, respectively. Of genes showing significant differences >2 fold, GOEA indicated that cellular and metabolic processes were highly represented. Expression of ANGPTL4, PLIN2, SQSTM1, and DDIT3 were significantly upregulated and HHIP was downregulated by rosiglitazone. CK5 was downregulated by rosiglitazone. Conclusions The RNA-seq data suggested that PPARγ activation induced alterations in cell differentiation and metabolic process and affected multiple signaling pathways such as PPAR, autophagy, WNT, and Hedgehog.

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A study of genes involved in adipocyte differentiation

Abstract: A variety of genes were discovered and validated with RT-PCR at the late phase of adipocyte differentiation. This may help us better understand the onset of obesity and the potential role of adipocytes in other organs.

Cited by 14 publications

References 7 publications

Angiopoietin-like protein 4 (ANGPTL4) is related to gestational weight gain in pregnant women with obesity

Angiopoietin-like protein 4 (ANGPTL4) is related to gestational weight gain in pregnant women with obesity

Eukaryotic LYR Proteins Interact with Mitochondrial Protein Complexes

Transcriptome analysis after PPARγ activation in human meibomian gland epithelial cells (hMGEC)

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