A Study of Factors Affecting the Success of Human Fertilization in Vitro. II. Influence of Semen Quality and Oocyte Maturity on Fertilization and Cleavage

Testart, J; Lassalle, Bruno; Frydman, René; Belaïsch, J

doi:10.1095/biolreprod28.2.425

Cited by 53 publications

(20 citation statements)

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“…The degree of cumulus expansion did not necessarily correlate with nuclear maturation, as the maturation rate of oocytes whose cumulus remained compact after IVM was not significantly different from that of oocytes whose cumulus became expanded during IVM. However, these two conditions are necessary, as Testart et al [58] found that a higher proportion of human oocytes were able to cleave following IVF when cumulus expansion was complete.…”

Section: Discussionmentioning

confidence: 99%

Equine Oocyte Competence for Nuclear and Cytoplasmic in Vitro Maturation: Effect of Follicle Size and Hormonal Environment1

Goudet

Bézard

Duchamp

et al. 1997

Biology of Reproduction

108

102

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Equine oocyte competence after in vitro maturation (IVM) was investigated in terms of the diameter of the follicle of origin and the stage of the estrous cycle, with three criteria of maturation: nuclear stage after DNA Hoechst staining, meiotic spindle morphology after tubulin immunocytochemical staining, and cortical granule localization after lectin labeling. Seven successive in vivo ultrasound-guided follicular punctures were performed on 10 cyclic saddle mares, alternatively at the end of the follicular phase (after induction of ovulation with a gonadotropin injection) and in midluteal phase (with or without a gonadotropin injection). Expanded cumulus-oocyte complexes (COCs) were stained at collection, and compact COCs were stained after in vitro culture. They were observed under a confocal microscope. Successive punctures on one mare provided 0.9 preovulatory COCs and 8 immature COCs per 22 days. Among the preovulatory oocytes, 55% had completed nuclear and cytoplasmic maturation, 86% of which displayed a normal meiotic spindle. Of the 262 oocytes cultured in vitro, 37% completed nuclear maturation. The nuclear and cytoplasmic maturation rate significantly increased with follicle diameter. The IVM rate tended to be higher in follicular phase and tended to increase in luteal phase with the gonadotropin injection. The meiotic spindle morphology was not significantly different between the classes of follicular diameters. This study provided the opportunity to increase the number of characterized oocytes collected per cycle and per mare. This is the first report showing the progressive acquisition of meiotic competence in the equine oocyte during antral follicle growth and is the only description of the equine meiotic spindle.

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Section: Discussionmentioning

confidence: 99%

Equine Oocyte Competence for Nuclear and Cytoplasmic in Vitro Maturation: Effect of Follicle Size and Hormonal Environment1

Goudet

Bézard

Duchamp

et al. 1997

Biology of Reproduction

108

102

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“…CUMULUS CELL miR-378 IMPAIRS OOCYTE MATURATION one of the primary measures of oocyte quality, creating a microenvironment favorable for nutrients and paracrine factors to reach the oocyte to alter gene expression and allow meiotic progression (52). During ovulation, the expansion of cumulus facilitates the release of a COC from the follicle and helps to maintain the viability of the oocyte during its passage in the oviduct (22,40).…”

Section: E530mentioning

confidence: 99%

MicroRNA-378 regulates oocyte maturation via the suppression of aromatase in porcine cumulus cells

Pan

Toms

Shen

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

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Pan B, Toms D, Shen W, Li J. MicroRNA-378 regulates oocyte maturation via the suppression of aromatase in porcine cumulus cells. Am J Physiol Endocrinol Metab 308: E525-E534, 2015. First published January 27, 2015; doi:10.1152/ajpendo.00480.2014.-We sought to investigate whether miR-378 plays a role in cumulus cells and whether the manipulation of miRNA levels in cumulus cells influences oocyte maturation in vitro. Cumulus-oocyte complexes (COCs) from ovarian follicles had significantly lower levels of precursor and mature miR-378 in cumulus cells surrounding metaphase II (MII) oocytes than cumulus cells surrounding germinal vesicle (GV) oocytes, suggesting a possible role of miR-378 during COC maturation. Overexpression of miR-378 in cumulus cells impaired expansion and decreased expression of genes associated with expansion (HAS2, PTGS2) and oocyte maturation (CX43, ADAMTS1, PGR). Cumulus cell expression of miR-378 also suppressed oocyte progression from the GV to MII stage (from 54 Ϯ 2.7 to 31 Ϯ 5.1%), accompanied by a decrease of growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15), zona pellucida 3 (ZP3), and CX37 in the oocytes. Subsequent in vitro fertilization resulted in fewer oocytes from COCs overexpressing miR-378 reaching the blastocyst stage (7.3 Ϯ 0.7 vs. 16.6 Ϯ 0.5%). miR-378 knockdown led to increased cumulus expansion and oocyte progression to MII, confirming a specific effect of miR-378 in suppressing COC maturation. Aromatase (CYP19A1) expression in cumulus cells was also inhibited by miR-378, leading to a significant decrease in estradiol production. The addition of estradiol to IVM culture medium reversed the effect of miR-378 on cumulus expansion and oocyte meiotic progression, suggesting that decreased estradiol production via suppression of aromatase may be one of the mechanisms by which miR-378 regulates the maturation of COCs. Our data suggest that miR-378 alters gene expression and function in cumulus cells and influences oocyte maturation, possibly via oocyte-cumulus interaction and paracrine regulation.

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“…Evidence has emerged to support the involvement of various locally produced factors as co-regulators of folliculogenesis and oocyte nuclear and cytoplasmic maturation in addition to extrinsic regulation by pituitary gonadotropins and metabolic hormones [5]. Optimal expansion of the cumulus mass appears to be essential for cytoplasmic maturation [6,7]. In the bovine, the induction of cumulus expansion prior to fertilization increased the incidence of oocyte penetration [8].…”

Section: Introductionmentioning

confidence: 99%

Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis

Dell’Aquila

Caillaud

Maritato

et al. 2004

Reprod Biol Endocrinol

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The aim of this study was to investigate cumulus expansion, nuclear maturation and expression of connexin 43, cyclooxygenase-2 and FSH receptor transcripts in equine cumuli oophori during in vivo and in vitro maturation in the presence of equine FSH (eFSH) and precursors for hyaluronic acid synthesis. Equine cumulus-oocyte complexes (COC) were cultured in a control defined medium supplemented with eFSH (0 to 5 micrograms/ml), Fetal Calf Serum (FCS), precursors for hyaluronic acid synthesis or glutamine according to the experiments. After in vitro maturation, the cumulus expansion rate was increased with 1 microgram/ml eFSH, and was the highest with 20% FCS. It was not influenced by precursors for hyaluronic acid synthesis or glutamine. The expression of transcripts related to cumulus expansion was analyzed in equine cumulus cells before maturation, and after in vivo and in vitro maturation, by using reverse transcription-polymerase chain reaction (RT-PCR) with specific primers. Connexin 43, cyclooxygenase-2 (COX-2) and FSH receptor (FSHr) mRNA were detected in equine cumulus cells before and after maturation. Their level did not vary during in vivo or in vitro maturation and was influenced neither by FSH nor by precursors for hyaluronic acid synthesis. Results indicate that previously reported regulation of connexin 43 and COX-2 proteins during equine COC maturation may involve post-transcriptional mechanisms.

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A Study of Factors Affecting the Success of Human Fertilization in Vitro. II. Influence of Semen Quality and Oocyte Maturity on Fertilization and Cleavage

Abstract: The incidence of in vitro fertilizationwas analyzed with respect to the degree of cumulus dissociation (expansion) at the time of oocyte recovery and also the semen quality.Of the oocytes surrounded by perfectly ("++") or moderately ("+") p<0.ool.

Cited by 53 publications

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Equine Oocyte Competence for Nuclear and Cytoplasmic in Vitro Maturation: Effect of Follicle Size and Hormonal Environment1

Equine Oocyte Competence for Nuclear and Cytoplasmic in Vitro Maturation: Effect of Follicle Size and Hormonal Environment1

MicroRNA-378 regulates oocyte maturation via the suppression of aromatase in porcine cumulus cells

Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis

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