A study of efficacy of phage amplification technique in diagnosis of pulmonary and extra-pulmonary tuberculosis

Singh, Jai Pratap

doi:10.18203/2349-3933.ijam20190127

Cited by 1 publication

(1 citation statement)

References 2 publications

(2 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…76 FASTPlaqueTBTM, a commercially available kit, offers greater sensitivity and specificity than standard MTB diagnostic methods and can more precisely represent MTB activity, metabolic features, and the proportion of drugresistant bacteria. 77 The second type is Bronx Box, which is based on fluorescent reporter phage and can decrease the time of DST testing to three days for INH, RIF, capreomycin and EMB. Mycobacteriophage-based assays have several advantages, including low cost, reduced detection time, ease of use, infecting only living bacteria and decreased false positive results.…”

Section: Phage-based Susceptibility Testingmentioning

confidence: 99%

Tuberculosis Diagnosis and Detection of Drug Resistance: A Comprehensive Updated Review

Tawfick,

Badawy,

Taleb

et al. 2023

J. Pure Appl. Microbiol.

View full text Add to dashboard Cite

Tuberculosis (TB) is a significant public health challenge, especially in developing nations. Developing a TB eradication strategy is hampered by the global health concern of drug-resistant (DR) TB. Effective patient treatment, preventing TB transfer and avoiding the upsurge of DR strains depend primarily on the timely and accurate identification of DR TB. Due to inadequate sensitivity, the necessity of trained laboratory personnel, the sluggish growth pattern of Mycobacterium bacilli in culture, and the small number of bacilli that are usually found in extrapulmonary TB samples, TB diagnosis is still tricky in clinical practice. Although mycobacterial culture is the gold standard to identify TB and determine drug resistance, it takes 2 to 8 weeks to develop. Despite their high cost, nucleic acid amplification tests (NAATs) and whole-genome sequencing (WGS) are the commonly employed molecular-based methods for diagnosing and identifying TB. The WHO suggested the GeneXpert MTB/RIF to identify TB and detect resistance to rifampicin. In comparison, numerous molecular techniques were developed, including allele-specific PCR (MAS-PCR), solid-phase hybridization, real-time PCR (RT-PCR) and droplet digital PCR-based technique (DDPCR). This manuscript is intended to overview the current approaches for the phenotypic and genotypic diagnosis of TB disease and identifying resistance to antitubercular drugs depending on recently published articles, WHO and CDC reports, and commercially available diagnostic tools.

show abstract

Section: Phage-based Susceptibility Testingmentioning

confidence: 99%