A study of dielectric membrane breakdown in theFucus egg

Gauger, Bruno; Bentrup, Friedrich‐Wilhelm

doi:10.1007/bf01872894

Cited by 32 publications

(8 citation statements)

References 24 publications

(53 reference statements)

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“…The effect of high voltage discharges on cells is to generate holes in the plasma membrane, presumably by causing localized dielectric breakdown. 21 " 23 We confirmed that the exposure of islets to the shocking regimen increases the permeability to small molecular weight ions by observing the efflux of 86 Rb + ( Figure 2). Islets preloaded with 86 Rb + released 92% of the radioisotope within 1 min as compared with only a 55% loss from islets not subjected to the shocking regimen.…”

Section: Resultssupporting

confidence: 87%

Use of a High Voltage Technique to Determine the Molecular Requirements for Exocytosis in Islet Cells

et al. 1980

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Pancreatic islet cells were subjected to high voltage discharges, which induced pore formation in the plasma membrane. This technique was used to determine the molecular requirements of intracellular sites involved in the control of insulin release in /3-cells. Islets, preloaded with 86 Rb + and then shocked, released 92% of the radioisotope within 1 min as compared with only a 55% loss from nonshocked islets. Exposure of the islets to 14 C-urea and 3 H-sucrose at 0 to 10 min after exposure to high voltage discharges indicated that 68% of the intracellular space was occupied by sucrose, whereas sucrose was excluded from nonshocked islet cells. The pores in the plasma membrane resealed about 30 min after their initial formation, as was indicated by the cellular exclusion of sucrose.The Ca ++ concentrations yielding half-maximal and maximal secretory responses from shocked islets were 0.05 x 10~6 M and 0.35 x 1O~6 M, respectively; the presence of 16.7 mM glucose did not alter these values. In intact islets, a variation of extracellular Ca ++ (only in the presence of 16.7 mM glucose) generated a dose-response curve yielding half-maximal and maximal secretory responses at 2.0 x 10~3 M and 4.0 x 1O~3 M, respectively. The total amount of insulin released from shocked islets was three times that released from nonshocked islets during a 15 min incubation period. The addition of 1 or 5 mM ATP during an initial shock and incubation period did not augment the secretory response to 0.05 x 10 6 M Ca ++ , but the presence of ATP was necessary, or the islets would not respond to 0.35 x 1 0 6 M Ca ++ during a subsequent shock and incubation period. The presence of 1.0 mM 3-phosphoglycerate or phosphoenolpyruvate augmented the secretory response to 0.05 x 10~6 M Ca ++ only in the presence of 1.0 mM ATP. Glucose-6-phosphate or fructose-1,6-diphosphate had no influence on the secretory response to Ca ++ in the presence of ATP. An increase in Mg ++ from 1.0 to 10 mM reduced the secretory response to 0.35 x 10~6 M Ca ++ by 63%. Islets, subjected to the high voltage discharges and allowed 30 min to reseal, exhibited a normal secretory response to 16.7 mM glucose. The results indicate that the high voltage technique induces reversible pore formation in /3-cells to introduce ions and solutes into the intracellular environment so that the factors controlling exocytosis can be determined. DIABETES 29:911-918, November 1980.

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Section: Resultssupporting

confidence: 87%

Use of a High Voltage Technique to Determine the Molecular Requirements for Exocytosis in Islet Cells

et al. 1980

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“…The ability of cellular membranes to recover from milder electrical pulses in what is now called "reversible electroporation" was first elucidated in the 1970s. 4,22,28 In 1993, Salford et al 47 first reported on the combined use of electropermeabilization and bleomycin in a rodent glioma model, demonstrating a doubling in survival time compared with animals treated with bleomycin alone. To irreversibly electroporate cell membranes, the electric field in the targeted region needs to be above a critical value, which is dependent on a variety of conditions such as tissue type and pulse parameters (amplitude, duration, frequency, and number).…”

Section: Discussionmentioning

confidence: 99%

Nonthermal irreversible electroporation for intracranial surgical applications

Ellis

García

Rossmeisl

et al. 2011

JNS

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Object. Nonthermal irreversible electroporation (NTIRE) is a novel, minimally invasive technique to treat cancer, which is unique because of its nonthermal mechanism of tumor ablation. This paper evaluates the safety of an NTIRE procedure to lesion normal canine brain tissue.Methods. The NTIRE procedure involved placing electrodes into a targeted area of brain in 3 dogs and delivering a series of short and intense electric pulses. The voltages of the pulses applied were varied between dogs. Another dog was used as a sham control. One additional dog was treated at an extreme voltage to determine the upper safety limits of the procedure. Ultrasonography was used at the time of the procedure to determine if the lesions could be visualized intraoperatively. The volumes of ablated tissue were then estimated on postprocedure MR imaging. Histological brain sections were then analyzed to evaluate the lesions produced.Results. The animals tolerated the procedure with no apparent complications except for the animal that was treated at the upper voltage limit. The lesion volume appeared to decrease with decreasing voltage of applied pulses. Histological examination revealed cell death within the treated volume with a submillimeter transition zone between necrotic and normal brain.Conclusions. The authors' results reveal that NTIRE at selected voltages can be safely administered in normal canine brain and that the volume of ablated tissue correlates with the voltage of the applied pulses. This preliminary study is the first step toward using NTIRE as a brain cancer treatment.

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“…Later, the concept of 'membrane pore' formation, as the result of dielectric breakdown of the cell membranes, was suggested by Kinosita and Tsong [27]. It was also shown that when short duration electric pulses were applied, the cell membranes may reseal [7,8,17,72].…”

Section: Experimental Studies Of Electroporationmentioning

confidence: 99%

Membrane electroporation theories: a review

et al. 2006

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Electroporation, the transient increase in the permeability of cell membranes when exposed to a high electric field, is an established in vitro technique and is used to introduce DNA or other molecules into cells. When the trans-membrane voltage induced by an external electric field exceeds a certain threshold (normally 0.2-1 V), a rearrangement of the molecular structure of the membrane occurs, leading to pore formation in the membrane and a considerable increase in the cell membrane permeability to ions, molecules and even macromolecules. This phenomenon is, potentially, the basis for many in vivo applications such as electrochemotherapy and gene therapy, but still lacks a comprehensive theoretical basis. This article reviews the state of current electroporation theories and briefly considers current and potential applications in biology and medicine.

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A study of dielectric membrane breakdown in theFucus egg

Cited by 32 publications

References 24 publications

Use of a High Voltage Technique to Determine the Molecular Requirements for Exocytosis in Islet Cells

Use of a High Voltage Technique to Determine the Molecular Requirements for Exocytosis in Islet Cells

Nonthermal irreversible electroporation for intracranial surgical applications

Membrane electroporation theories: a review

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