A study of cotyledon senescence in cucumber (Cucumis sativus L.) based on expressed sequence tags and gene expression

Kim, Dae Jae

doi:10.1007/bf03030515

Cited by 9 publications

(17 citation statements)

References 49 publications

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“…Cucumber cotyledons offer a potentially ideal model system for this type of experiment, since one cotyledon leaf of a plant could be subjected to mineral analysis while the second one is harvested for RNA isolation. ESTs from senescing cucumber cotyledons have been described (Kim 2004), and with the completion of the cucumber genome (Huang et al 2009) genome-wide transcriptional profiling experiments are now feasible.…”

Section: Discussionmentioning

confidence: 99%

Natural variation in iron use efficiency and mineral remobilization in cucumber (Cucumis sativus)

Waters

Troupe

2011

Plant Soil

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Section: Discussionmentioning

confidence: 99%

Natural variation in iron use efficiency and mineral remobilization in cucumber (Cucumis sativus)

Waters

Troupe

2011

Plant Soil

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“…Previously, we proposed that the cucumber PEPCK and ICL genes function in senescing the cotyledon for recycling of the demolished intracellular organelles and supply their nutrients to other parts of the cell [6,8]. However, a different mechanism was proposed for ICL and PEPCK enzyme involvement in senescing organs that also needs to be elucidated in the future [1].…”

Section: Discussionmentioning

confidence: 99%

Metabolic Gene Expression in Lipid Metabolism during Cotyledon Development in Cucumbers and the Possibility of a Secondary Transport Route of Acetyl Units

Jeong¹,

Kim

2014

Journal of Life Science

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We investigated the expression of cucumber genes involved in lipid mobilization and metabolism during cotyledon development to compare gene activity and to study the direction of carbon (acetyl unit) transport between glyoxysomes and mitochondria. The core metabolic pathway involving 10 genes was examined in four intracellular compartments: glyoxysomes (peroxisomes), mitochondria, chloroplasts, and cytosol. Additionally, we tested the early germination response of dark-grown seedlings and the immediate light response for a further 3 days. According to the reverse transcription polymerase chain reaction (RT-PCR), 3-L-ketoacyl-CoA thiolase 2 (Thio2), isocitrate lyase (ICL), and malate synthase (MS), the genes involved in storage lipid mobilization showed a similar and consistent pattern of gene expression in seedling development. Furthermore, coordinate expression of the A BOUT DE SOUFFLE (BOU) gene with ICL and MS during seedling emergence pointed to a possible secondary route of acetyl unit (acetyl-CoA) transport between peroxisomes and mitochondria in cucumber. The expression of the BOU gene was light dependent, as shown by BOU activity in Arabidopsis, suggesting that the dark condition also results in weak membrane biogenesis. In addition, several genes were active throughout the development of the green cotyledon, even during senescence. In conclusion, this study summarizes oil-seed germination and gene expression during cucumber cotyledon development and proposes an additional route for acetyl unit transport.

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“…Genes and bioinformatics analysis Cucumber SAGs were presented in Table 1 been isolated from previous study [19]. However, we have cloned more than hundreds of cDNA clones which should be further identified the possible biological functions from the bioinformatics study through National Center for Biotechnology Information (NCBI) (Data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…Polymerase chain reaction (PCR) was performed for 30 cycles using MyCycler (BioRad, USA) with an AccuPower PCR PreMix Kit (Bioneer, Korea) by gene-specific primers (5'-3'), as described in a previous report [19]. All primers were manufactured through Bioneer Co (Daejeon, Korea).…”

Section: Reverse Transcription (Rt) Reaction and Pcr For Cucumber Genesmentioning

confidence: 99%

“…Also, senescence has been studied in many plant species [7,19,20,28,29]. Although senescence occurs in an age-dependent mode in many species, the initiation and progression of senescence can be modulated by a variety of environmental factors (exogenous and endogenous stimulus), such as temperature, mineral deficiency, drought conditions, and pathogen infections following nutrients, hormone and light stress.…”

Section: Introductionmentioning

confidence: 99%

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Response to Plant Hormones of Senescence-related Genes for Cucumis sativus L. in Cotyledon Development

Jeong¹,

Kim²

2016

Journal of Life Science

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This study was carried out to discover the response of cucumber (Cucumis sativus L.) senescence-associated genes (SAGs) to several plant hormones in detached and developing cotyledon. Accordingly, a collection of cucumber SAGs were examined to characterize their gene expression response through semi-quantitative RT-PCR. Cotyledons were excised at day 14 after seed sowing from plantlets, then incubated in 100 μM each of IAA or zeatin solution for up to 4 days in light and darkness. They were collected at 2-day intervals and used for total RNA extraction and subjected to RT-PCR. Gene expression levels of several cucumber SAGs were significantly changed during the incubation period. More than five cucumber SAGs involving SAG 60 responded to the IAA and zeatin treatment. In the ethylene response study, cotyledons were exposed up to 10 days by ethylene gas. Most of the cucumber SAGs did not show immediate response to ethylene in green cotyledon. The exceptions were PCK, SAG 158, and SAG 288 genes, which responded after 1 day of exposure to green cotyledon, while ICL and SAG 281 revealed strong responses after 10 days of being exposed to yellowing cotyledon. These results suggest that several cucumber SAGs react actively in response to starvation or senescence against exogenously applied stimulus. This induced senescence response is able to understand the SAGs role in lipids and amino acids metabolism partly and function in organ senescence during development.

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A study of cotyledon senescence in cucumber (Cucumis sativus L.) based on expressed sequence tags and gene expression

Cited by 9 publications

References 49 publications

Natural variation in iron use efficiency and mineral remobilization in cucumber (Cucumis sativus)

Natural variation in iron use efficiency and mineral remobilization in cucumber (Cucumis sativus)

Metabolic Gene Expression in Lipid Metabolism during Cotyledon Development in Cucumbers and the Possibility of a Secondary Transport Route of Acetyl Units

Response to Plant Hormones of Senescence-related Genes for Cucumis sativus L. in Cotyledon Development

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