A study of chemically induced dynamic electron polarization (CIDEP) in Photosystem I of whole algal cells at ambient temperatures

Manikowski, H.; McIntosh, Alan R.; Bolton, James R.

doi:10.1016/0005-2728(84)90158-0

Cited by 12 publications

(10 citation statements)

References 32 publications

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“…This loss of structure going from 2 H to *H material was also observed in the ESE work of Norris et al (1978), see below. Manikowski et al (1984) observed that, when the temperature was lowered from 293 to 273 K, the 1 /is signal became close to the signal observed at cryogenic temperatures in unorientated samples (see Section 3.2.2). This resolves the hitherto puzzling fact that the nonflow signal at room temperature of could not be reproduced for unorientated frozen samples.…”

Section: Q* 9i = */»" • (Ip+-lx-) • ( V -^X -) = Q V (3-3)supporting

confidence: 66%

“…It was assumed that in part of the reaction centres forward electron transport to X was blocked by the repetitive flash technique. Unfortunately, the inaccuracy of the field profile of the fast phase makes it difficult to compare it with the spectrum obtained by Manikowski et al (1984). The 00-08 /is component showed a largely emissive signal under non-flow conditions.…”

Section: Q* 9i = */»" • (Ip+-lx-) • ( V -^X -) = Q V (3-3)mentioning

confidence: 99%

“…This resolves the hitherto puzzling fact that the nonflow signal at room temperature of could not be reproduced for unorientated frozen samples. The work of Dismukes & Tycko (1981) and Manikowski et al (1984) demonstrates that the 'purely' emissive signal is not a( = o polarized spectrum so that it cannot be used for the type of spectral simulation employed by Friesner et al (1979). The 273 K, 1 /is spectrum of Manikowski et al (1984) further demonstrates that cw, 1 MHz modulation detection essentially gives the same results as cw direct detection.…”

Section: Q* 9i = */»" • (Ip+-lx-) • ( V -^X -) = Q V (3-3)mentioning

confidence: 99%

“…Incident microwave power, 20 mW; temperature, 273 K. (B) As (A) for T = 293 K. (C) As (A) for protonated Anacystis nidulans algae. (D) As (C) for T = 293 K. FromManikowski et al (1984). (A) Time-resolved ESE spectra of perdeuterated Synechococcus lividus algae at room temperature.…”

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confidence: 99%

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Electron spin polarization of photosynthetic reactants

Hoff

1984

Quart. Rev. Biophys.

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Photosynthesis is the conversion of the quantum energy of light into the chemical energy of complex organic molecules and organized cellular structures in plants and in some bacteria. The processes of photosynthesis span the time domain of subpicoseconds to the millennia of slow-growing trees, its study brings together such diverse disciplines as photophysics, biochemistry, botany and ecology. In the last few decades tremendous progress has been made in understanding the multivarious chemical reactions that ultimately lead to the fixation of carbon dioxide into organic substance, yet the basic mechanism underlying the conversion of photon energy into chemical energy still remains very much an enigma. These so-called primary reactions which transduce the excitation energy of excited chlorophyll pigments into the potential energy of stabilized, separated charges on electron donor and electron acceptor molecules have been studied with a variety of physical techniques, among which fast optical spectroscopy and electron paramagnetic resonance (EPR) are prominent. This review will highlight one intriguing aspect of EPR, namely that of electron spin polarization (ESP).† It will be shown that ESP of photosynthetic primary reactants offers a unique tool to gain insight in the electrostatic and magnetic interactions that make photosynthesis work. Moreover, it will become apparent that ESP in photosynthesis has several unique traits not (yet) found in ESP of photochemical reactions in vitro. As such, it may serve as a paradigma of ESP phenomena and will present an absorbing spectacle also for EPR spectroscopists outside photosynthesis.

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Section: Q* 9i = */»" • (Ip+-lx-) • ( V -^X -) = Q V (3-3)supporting

confidence: 66%

Section: Q* 9i = */»" • (Ip+-lx-) • ( V -^X -) = Q V (3-3)mentioning

confidence: 99%

Section: Q* 9i = */»" • (Ip+-lx-) • ( V -^X -) = Q V (3-3)mentioning

confidence: 99%

mentioning

confidence: 99%

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Electron spin polarization of photosynthetic reactants

Hoff

1984

Quart. Rev. Biophys.

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“…It was found that the polarized lineshape depended greatly on orientation of the chloroplasts (Dismukes et al, 1978;Dismukes and Sauer, 1978). Early attempts at simulation (Friessner et al, 1979) of the lineshape of the orientated and unorientated material were invalidated when later work established that the lineshape of unorientated chloroplasts at early times was more complicated than found earlier (Dismukes and Tycko, 1981;Manikowski et al, 1984). This result was confirmed and extended by time-resolved EPR at higher frequencies of deuterated algae [24 GHz (Furrer and Thurnauer, 1983); 35 GHz (Thurnauer and Gast, 1985)l.…”

Section: The Plant Photosystemsmentioning

confidence: 95%

Magnetic Interactions Between Photosynthetic Reactants

Hoff

1986

Photochem & Photobiology

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Experimental Evidence for the Anisotropic Nature of the Transient EPR Spectrum from Photosystem I Observed in Cyanobacteria

Bock

Stehlik

Thurnauer

1988

Israel Journal of Chemistry

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Abstract. Early transient EPR spectra at room temperature, with high spectral (Kband, 24 GHz) and time (50 ns) resolution, are presented for whole cells of deuterated cyanobacteria (Synechococcus lividus). A full range, between high and low microwave power, is covered. The true low-power spectrum of the charge-separated state in Photosystem I prior to electron transfer to the iron-sulfur center acceptors could be established. The anisotropic nature of the signal has been confirmed with the observation of transient nutations at high microwave power, which indicate possible motional effects or spin dynamics.

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A study of chemically induced dynamic electron polarization (CIDEP) in Photosystem I of whole algal cells at ambient temperatures

Cited by 12 publications

References 32 publications

Electron spin polarization of photosynthetic reactants

Electron spin polarization of photosynthetic reactants

Magnetic Interactions Between Photosynthetic Reactants

Experimental Evidence for the Anisotropic Nature of the Transient EPR Spectrum from Photosystem I Observed in Cyanobacteria

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