A Study of Bromine Speciation in Human Serum and Ambroxol Determination in Rat Plasma by Liquid Chromatography–Inductively Coupled Plasma Mass Spectrometry

Liu, Deye; Chen, Geng; Huo, Zongli; Hong, Liu; Ji, Wenling; Liu, Hualiang

doi:10.1007/s10337-019-03730-z

Cited by 9 publications

(1 citation statement)

References 36 publications

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“…Iodine can only be obtained from the outside world, and the iodine content in food is extremely low. Te resulting iodine defciency causes goiter and afects the intelligence and physical development of the human body [1][2][3]. Te bioavailability and toxicity of diferent forms of iodine are diferent, and the toxicity of iodate is more signifcant than iodide.…”

Section: Introductionmentioning

confidence: 99%

Trypsin Digestion Pretreatment of Kelp Samples and the Iodine Speciation Analysis of Iodate, Iodide, MIT, and DIT by HPLC-ICP-MS

Chen

Cui

et al. 2023

Journal of Chemistry

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The analysis of iodine content in kelp is mainly focused on determining total iodine content, which lacks a rapid and sensitive analytical method for iodine species corresponding to iodate, iodide, monoiodotyrosine (MIT), and diiodo-tyrosine (DIT). Based on exploring the pretreatment methods of enzymatic hydrolysis of kelp, a rapid determination method of high-performance liquid chromatography and inductively coupled plasma mass spectrometry (HPLC-ICP-MS) for different iodine species existed in kelp samples was established. The pretreatment method is trypsin hydrolysis of kelp samples treated at 50°C for 10 hours, then the extraction solution was obtained by centrifugation, and filtration was proved for the first time. A high-efficiency separation anion-exchange column (Dionex IonPac AS-14) for iodate, iodide, MIT, and DIT with 0.1 mol·L−1(NH4)2CO3 solution at pH = 9 as a mobile phase, a flow rate of 0.8 mL·min−1 for the first 1500 s, and 1.2 mL·min−1 for the last 1800 s was confirmed. The linear ranges of the standard calibrations on four iodine species are 1.0–150 μg·L−1, and the detection limits are 1.62, 1.02, 1.59, and 2.55 μg·L−1, respectively, while the RSD values of four iodine species are all less than 2%. Under the optimized conditions, the proposed method was successfully applied for the speciation analysis of iodine in kelp samples.

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Section: Introductionmentioning

confidence: 99%