A study of blood flow dynamics in flap delay using the full-field laser perfusion imager

Du, Zijing; Zan, Tao; Li, Hua; Li, Qingfeng

doi:10.1016/j.mvr.2011.09.010

Cited by 20 publications

(19 citation statements)

References 41 publications

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“…FLPI were measured under anesthesia using The Moor instrument (Axminster, UK) as described previously [25]. Briefly, the FLPI measurements were performed in a warm and quiet environment.…”

Section: Methodsmentioning

confidence: 99%

“…To evaluate microvessel density, we first stained tissue samples with the endothelial cell marker von Willebrand Factor [25,26]. In brief, rats were anesthetized and full thickness skin flap specimens measuring 0.5 cm × 0.5 cm from proximal margin 3 cm from the base were harvested, fixed in 10% saline-buffered formalin, embedded in paraffin, and cut into 5-µm-thick sections.…”

Section: Methodsmentioning

confidence: 99%

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Isoflurane Preconditioning Increases Survival of Rat Skin Random-Pattern Flaps by Induction of HIF-1a Expression

Sun

Zhang

et al. 2013

Cell Physiol Biochem

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Background: Survival of random-pattern skin flaps is important for the success of plastic and reconstructive surgeries. This study investigates isoflurane-induced protection against ischemia of skin flap and the underlying molecular mechanism in this process. Methods: Human umbilical vein endothelial cells (HUVECs) and human skin fibroblast cells were exposed to isoflurane for 4 h. Expression of hypoxia inducible factor-1α (HIF-1α), heme oxygenase-1 (HO-1) and vascular endothelial growth factor (VEGF) were analyzed up to 24 h post isoflurane exposure using qRT-PCR and western blot, or ELISA analyses. PI3K inhibitors - LY 294002 and wortmannin, mTOR inhibitor - rapamycin, and GSK3β inhibitor - SB 216763 were used respectively to assess the effects of isoflurane treatment and HIF-1α expression. Furthermore, 40 rats were randomly divided into 5 groups (control, isoflurane, scrambled siRNA plus isoflurane, HIF-1α siRNA plus isoflurane, and DMOG) and subjected to random-pattern skin flaps operation. Rats were prepared for evaluation of flap survival and full-feld laser perfusion imager (FLPI) (at 7 day) and microvessel density evaluation (at 10 day). Results: Isoflurane exposure induced expression of HIF-1α protein, HO-1 and VEGF mRNA and proteins in a time-dependent manner. Both LY 294002 and wortmannin inhibited phospho-Akt, phospho-mTOR, phospho-GSK 3β and HIF-1α expression after isoflurane exposure. Both wortmannin and rapamycin inhibited isoflurane-induced phospho-4E-BP1 (Ser 65) and phospho-P70^s6k (Thr 389) and HIF-1α expression. SB 216763 pre-treatment could further enhance isoflurane-induced expression of phospho-GSK 3β (Ser 9) and HIF-1α protein compared to the isoflurane-alone cells. In animal experiments, isoflurane alone, scrambled siRNA plus isoflurane, or DMOG groups had significantly upregulated vascularity and increased survival of the skin flaps compared to the controls. However, HIF-1α knockdown abrogated the protective effect of isoflurane preconditioning in rats. Conclusions: Isoflurane preconditioning improves survival of skin flaps by up the regulation of HIF-1α expression via Akt-mTOR and Akt-GSK 3β signaling pathways.

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“…FLPI were measured under anesthesia using The Moor instrument (Axminster, UK) as described previously [25]. Briefly, the FLPI measurements were performed in a warm and quiet environment.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Isoflurane Preconditioning Increases Survival of Rat Skin Random-Pattern Flaps by Induction of HIF-1a Expression

Sun

Zhang

et al. 2013

Cell Physiol Biochem

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“…Although accurate absolute measurements RBC V cannot be calculated, perfusion changes can be successively monitored and tracked. Laser speckle imaging has been demonstrated on the rodent skin fold model [119,120], assessed the vasodilation responses of intestinal tissues [121], analysed retinal blood flow and cerebral perfusion [122][123][124][125], and stroke [110].…”

Section: Laser Speckle Imagingmentioning

confidence: 99%

“…Reported studies have shown it to be used in the quantitative analysis of cancer metastatic growth [208,209] and in the detection of hypoxic cells [210]; it has provided contrast in ocular surgery [211]; qualitative assessment of its use in esophageal lesions has been reported [212]; and analysis of irregular pigmentation, for example, port wine stains [213][214][215]. Histology has also been employed for flow purposes, for example, in the observation of novel threadlike structures on organ surfaces [216] and has been used in conjunction with LSI (see § 2.2.3) to visualise blood vessel density [120].…”

Section: Histological Stainingmentioning

confidence: 99%

“…OPT imaging is performed in both transmission and emission modes; transmission is used for studies of anatomy, whereas emission mode is used to image the distribution of fluorescently labelled samples. In transmission mode, a wide field (diffuse or non-diffuse) light source illuminates a sample which is angu- Figure 17 Haematoxylin and eosin stained sections (1 Â 100) of rodent dorsal skin flap [120]. larly rotated stepwise; the light transmitted in this stepwise fashion is captured using a CCD microscope (see Figure 18).…”

Section: Optical Projection Tomographymentioning

confidence: 99%

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‘Go with the flow ’: A review of methods and advancements in blood flow imaging

Daly

Leahy

2012

Journal of Biophotonics

107

108

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Physics has delivered extraordinary developments in almost every facet of modern life. From the humble thermometer and stethoscope to X‐Ray, CT, MRI, ultrasound, PET and radiotherapy, our health has been transformed by these advances yielding both morphological and functional metrics. Recently high resolution label‐free imaging of the microcirculation at clinically relevant depths has become available in the research domain. In this paper, we present a comprehensive review on current imaging techniques, state‐of‐the‐art advancements and applications, and general perspectives on the prospects for these modalities in the clinical realm. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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Stem cells enhance reperfusion following ischemia: Validation using laser speckle imaging in predicting tissue repair

Tang¹,

Thompson²,

Nathan³

et al. 2018

The Laryngoscope

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A study of blood flow dynamics in flap delay using the full-field laser perfusion imager

Cited by 20 publications

References 41 publications

Isoflurane Preconditioning Increases Survival of Rat Skin Random-Pattern Flaps by Induction of HIF-1a Expression

Isoflurane Preconditioning Increases Survival of Rat Skin Random-Pattern Flaps by Induction of HIF-1a Expression

‘Go with the flow ’: A review of methods and advancements in blood flow imaging

Stem cells enhance reperfusion following ischemia: Validation using laser speckle imaging in predicting tissue repair

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