A study of 24 patients with colistin resistant gram negative isolates in a tertiary care hospital in South India

Abstract

VISA strains), with no totally vancomycin-resistant (VRSA) strains. All of phenotypic MRSA isolates, except of one, were positive for PBP2a. Conclusion:The study concludes that S. aureus is the most prevalent pathogen causing bacteremia in leukemia patients, with MRSA variety comprising the majority of these strains. Also, phenotypic method for MRSA detection can be performed using either of Methicillin, Oxacillin, or Cefoxitin with same results, with a nonsignificant statistical difference between the phenotypic method and the genotypic method-via the PBP2a detection.

Molecular study of colistin resistant clinical isolates of Enterobacteriaceae species

“…In the present study, 50 colistin resistant isolates during 36 months had been collected in which the most common sources were urine (46%), blood (30%) and wounds (24%).Similar result was obtained by previous study [22][23][24].…”

A comparative study among different methods of detection of colistin resistant gram negative bacilli in a tertiary care hospital

“…Elderly patients admitted to the intensive care units are at high risk from colistin resistant bacteria owing to reduced immunity and multiple co-morbidities. It was observed that the mean age of the patients was 55.2 year in the current study which was quite similar to observation by Arjun et al [9] and Goel et al [10] where they mentioned mean age of 58.33 year and 41.1 year respectively. However another studies [22,23] observed mean age of 23.5 years and 32.3 years which was a contrasting observation.…”

“…The various samples with colistin resistance organisms in the present study was seen maximum in endotracheal tube secretion (34.3%), urine (18.7%) followed by bronchial wash (12.5%), pus (12.5%), blood (9.3%) and sputum (6.25%) which was comparable to the study by Bhasker et al [24] where they also found colistin resistance organisms maximum in tracheal secretion (33.73%) followed by blood (24.3%), urine (17.9%), pus (6.7%), bronchoalveolar lavage (6.4%), catheter tips (4.5%), fluid (3.95%) and tissue (2.4%). In the study by Arjun et al [9] also found urine sample (33%) as a major source of colistin resistant isolate followed by respiratory samples (20.8%), pus (16.67%), blood (6%) and also in cerebrospinal fluid (4.17%) whereas Pawar et al [8] reported colistin resistant bacilli from pus (42.3%), followed by catheter tip (19.7%), urine (16.7%), and endotracheal tube secretion (9.1%), sputum (9.1%) each. Zaki et al [25] reported maximum colistin resistant isolates from urine (46%) followed by blood (30%) and exudates (24%).…”

“…The highest resistance was reported from Asia followed by Europe, America and Africa [3]. In India reports of colistin resistance among GNB are also emerging at an alarming rate especially colistin resistant Klebsiella pneumonia and Pseudomonas aeruginosa [8][9][10][11][12]. Due to emergence of MDR GNB colistin has been re-introduced as a therapeutic drug which can be used in both human and veterinary medicine [13,14].…”

“…Contributory factors such as recent hospitalization, prolonged hospital stay (24-25 days), surgical trauma, presence of infection and co-morbid conditions such as diabetes, chronic kidney disease (CKD), use of invasive devices and prior colistin exposure are commonly seen [9].…”