A study into parental assignment of the communal spawning protogynous hermaphrodite, giant grouper (Epinephelus lanceolatus)

Bright, David; Reynolds, Adam; Nguyen, Nguyen Hong; Knuckey, Richard; Knibb, Wayne; Elizur, Abigail

doi:10.1016/j.aquaculture.2016.03.013

Cited by 10 publications

(7 citation statements)

References 36 publications

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“…We also found the similar skew phenomenon in previous parentage research on orange-spotted grouper ( E. coioides ) [ 53 ]. This result is unexpected, since Bright et al [ 49 ] have described that, although a skew parental contribution exists in communally spawning giant grouper, all males and females successfully participated in production over the spawning period. The most possible explanation is that our sampling frequency of offspring was incomplete relative to the whole spawning periods, which could lead to a loss of genetic diversity and low parental contribution [ 49 ].…”

Section: Discussionmentioning

confidence: 92%

“…Whether in conservation or aquaculture programs of this species, the application of appropriate molecular tools to trace pedigree information will help to monitor the genetic diversity of wild or cultured population and thus inform researchers of adjusting management plans. However, although 72 microsatellite markers have been developed for giant grouper so far [ 43 , 45 , 46 , 47 ], only a few of them have been applied in parentage related analysis [ 48 , 49 ]. For groupers, moreover, the development of SNP markers has only been reported for identification of growth traits in potato grouper ( E. tukula ) [ 50 ] and orange-spotted grouper ( E. coioides ) [ 51 ], while the application of SNPs for parentage analysis in groupers has not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

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Parentage Analysis in Giant Grouper (Epinephelus lanceolatus) Using Microsatellite and SNP Markers from Genotyping-by-Sequencing Data

Weng

Yang

Wang

et al. 2021

Genes

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Pedigree information is necessary for the maintenance of diversity for wild and captive populations. Accurate pedigree is determined by molecular marker-based parentage analysis, which may be influenced by the polymorphism and number of markers, integrity of samples, relatedness of parents, or different analysis programs. Here, we described the first development of 208 single nucleotide polymorphisms (SNPs) and 11 microsatellites for giant grouper (Epinephelus lanceolatus) taking advantage of Genotyping-by-sequencing (GBS), and compared the power of SNPs and microsatellites for parentage and relatedness analysis, based on a mixed family composed of 4 candidate females, 4 candidate males and 289 offspring. CERVUS, PAPA and COLONY were used for mutually verification. We found that SNPs had a better potential for relatedness estimation, exclusion of non-parentage and individual identification than microsatellites, and > 98% accuracy of parentage assignment could be achieved by 100 polymorphic SNPs (MAF cut-off < 0.4) or 10 polymorphic microsatellites (mean Ho = 0.821, mean PIC = 0.651). This study provides a reference for the development of molecular markers for parentage analysis taking advantage of next-generation sequencing, and contributes to the molecular breeding, fishery management and population conservation.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Parentage Analysis in Giant Grouper (Epinephelus lanceolatus) Using Microsatellite and SNP Markers from Genotyping-by-Sequencing Data

Weng

Yang

Wang

et al. 2021

Genes

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“…One recent study on the contribution of individual broodstock to spawning in E. lanceolatus revealed that both the maternal and paternal contributions varied between different nights of spawning, and although all males and females successfully mated over the spawning period, the majority of offspring were from a single, dominant male in the broodstock population (Bright et al 2016). Clearly, studies such as these, incorporating molecular approaches, are valuable in providing information that can be used to infer social and behavioural aspects of grouper reproduction.…”

Section: Reproduction In Captivitymentioning

confidence: 99%

A review of grouper (Family Serranidae: Subfamily Epinephelinae) aquaculture from a sustainability science perspective

Rimmer

Glamuzina

2017

Reviews in Aquaculture

147

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Aquaculture of groupers is carried out in tropical and subtropical areas throughout the world, but most production is from Asia, with three countries responsible for an estimated 92% of global production: China (65% of total production), Taiwan Province of China (17%) and Indonesia (11%). We calculate that there are at least 47 grouper species plus 15 grouper hybrids that have been trialled or are currently aquacultured. While grouper aquaculture has undoubtedly provided positive social and economic benefits to coastal communities in Asia, practices associated with grouper aquaculture have also led to widespread concerns regarding its environmental impacts. This paper reviews environmental, economic and social impacts of grouper aquaculture within a sustainability science framework. An update, building on early technical reviews of grouper aquaculture, was used to identify the main environmental, economic and social impacts. The main environmental impacts identified were as follows: seedstock sources, particularly the use of wild‐caught postlarval or juvenile fish as seedstock; the use of ‘trash’ fish as the major feed source; and water quality and substrate impacts of sea cage aquaculture. Significant economic and social impacts arising from grouper aquaculture include employment and income generation, but the economic impacts of disease outbreaks are significant, reducing fish survival to harvest size to around 50–70%. Current approaches to ameliorate negative impacts include the following: development and implementation of better management practices (BMPs) at national level, and the development of a global ecolabel certification scheme by the Aquaculture Stewardship Council. Both these approaches have significant constraints, particularly when applied to small‐scale farmers who produce the bulk of farmed grouper. We conclude that there is a need for improved higher level coordination between the major producer countries to address identified sustainability constraints to grouper aquaculture.

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“…There are at least 47 grouper species plus 15 grouper hybrids that have been trialed or are currently aquacultured [1]. The giant grouper, Epinephelus lanceolatus , is the largest grouper in the world and can grow to 2.3 m, weigh up to 400 kg [3], and it is popular for its rapid growth, reaching up to 3 kg in the first year [4]. E. lanceolatus itself is difficult to breed and rear; therefore, incorporating the rapid growth rate of giant groupers in the genome of hybrids has been the major focus of research on hybrid groupers [5].…”

Section: Introductionmentioning

confidence: 99%

Whole Genome Sequencing of the Giant Grouper (Epinephelus lanceolatus) and High-Throughput Screening of Putative Antimicrobial Peptide Genes

et al. 2019

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Giant groupers, the largest grouper type in the world, are of economic importance in marine aquaculture for their rapid growth. At the same time, bacterial and viral diseases have become the main threats to the grouper industry. Here, we report a high-quality genome of a giant grouper sequenced by an Illumina HiSeq X-Ten and PacBio Bioscience Sequel platform. A total of 254 putative antimicrobial peptide (AMP) genes were identified, which can be divided into 34 classes according to the annotation of the Antimicrobial Peptides Database (APD3). Their locations in pseudochromosomes were also determined. Thrombin-, lectin-, and scolopendin-derived putative AMPs were the three largest parts. In addition, expressions of putative AMPs were measured by our transcriptome data. Two putative AMP genes (gapdh1 and gapdh2) were involved in glycolysis, which had extremely high expression levels in giant grouper muscle. As it has been reported that AMPs inhibit the growth of a broad spectrum of microbes and participate in regulating innate and adaptive immune responses, genome sequencing of this study provides a comprehensive cataloging of putative AMPs of groupers, supporting antimicrobial research and aquaculture therapy. These genomic resources will be beneficial to further molecular breeding of this economically important fish.

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A study into parental assignment of the communal spawning protogynous hermaphrodite, giant grouper (Epinephelus lanceolatus)

Cited by 10 publications

References 36 publications

Parentage Analysis in Giant Grouper (Epinephelus lanceolatus) Using Microsatellite and SNP Markers from Genotyping-by-Sequencing Data

Parentage Analysis in Giant Grouper (Epinephelus lanceolatus) Using Microsatellite and SNP Markers from Genotyping-by-Sequencing Data

A review of grouper (Family Serranidae: Subfamily Epinephelinae) aquaculture from a sustainability science perspective

Whole Genome Sequencing of the Giant Grouper (Epinephelus lanceolatus) and High-Throughput Screening of Putative Antimicrobial Peptide Genes

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