A Structures for Lossless Ion Manipulations (SLIM) Module for Collision Induced Dissociation

Abstract

A collision induced dissociation (CID) structures for lossless ion manipulations (SLIM) module is introduced and coupled to a quadrupole time-of-flight (QTOF) mass spectrometer (MS). The SLIM CID module was mounted after an ion mobility (IM) drift tube to enable IM/CID/MS studies. The efficiency of CID was studied by using the model peptide leucine enkephalin. CID efficiencies (62%) compared favorably to other beam-type CID methods. Additionally, the SLIM CID module was used to fragment a mixture of 9 peptides after IM separation. This work also represents the first application of SLIM in the 0.3 to 0.5 Torr pressure regime, an order of magnitude lower in pressure than previously studied.

Ion Mobility–Mass Spectrometry Using a Dual-Gated 3D Printed Ion Mobility Spectrometer

“…Glass drift tubes coated in a resistive material produce very uniform electric fields and act as an infinite number of closely spaced electrodes with continuous voltage drops along their central axes; however, such drift tubes are difficult to manufacture. Alternatively, drift tubes constructed using PCBs are simple to manufacture, inexpensive, and have been widely used, including in the construction of structures for lossless ion manipulations (SLIM), which cycles ions inside a serpentine-like drift tube multiple times, effectively achieving drift lengths several hundred meters in length. − As opposed to drift tubes, Barmpounis et al used mold casting to construct a DMA out of polyurethane and coated the pieces with a thin layer of nickel to provide electrical conductivity.…”

New frontiers for mass spectrometry based upon structures for lossless ion manipulations

“…Collision induced dissociation (CID) has already been illustrated with a constant field SLIM module operated at 265 mTorr. 55 The SLIM module consisted of three discrete but integrated regions. The first two regions had independent DC gradients and were followed by a region designed to act as a planar quadrupole.…”

“…(b) VCID = 30, CID efficiency = 62%. 55 Adapted with permission from ref. 55 , Copyright (2016) Springer.…”

Compression Ratio Ion Mobility Programming (CRIMP) Accumulation and Compression of Billions of Ions for Ion Mobility-Mass Spectrometry Using Traveling Waves in Structures for Lossless Ion Manipulations (SLIM)

“…We have been developing structures for lossless ion manipulations (SLIM) to address important analytical challenges. SLIM enable a palette of core capabilities that include IM separations, ion trapping, ion dissociation, and “switching” for ion selection for trapping or alternative routing. − Recently, very high IM resolution has been achieved using a 13 m long serpentine path SLIM module and initially demonstrated for a range of samples with lossless ion transmission. , This work has highlighted the potential for achieving ultrahigh IM resolution for efficient analyses of complex mixtures, and the recent development of multipass SLIM s erpentine u ltra-long p ath with e xtended r outing (SUPER) high-resolution IM-MS . However, the benefits of improved IM resolution using very long path lengths are limited at some point by increased peak broadening leading to peak dilution and consequent detection related degradation of sensitivity.…”