A Structural Genomics Approach to the Study of Quorum Sensing

Lewis, H.A.; Furlong, E.B.; Laubert, B.; Eroshkina, Galina; Batiyenko, Yelena; Adams, J.M.; Bergseid, M.G.; Marsh, C.D.; Peat, Thomas S.; Sanderson, Wendy; Sauder, J.M.; Buchanan, Sean G.

doi:10.1016/s0969-2126(01)00613-x

Cited by 88 publications

(88 citation statements)

References 22 publications

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“…We searched the Protein Data Bank to find a protein possessing a cysteine and several histidines positioned adjacent to each other in a similar manner as in PH1602-extein. The search results included several zinc metalloenzymes, such as LuxS quorum-sensing proteins, 15 and some tRNA synthetases. 16 Indeed, zinc ions show a stronger preference toward nitrogen and sulfurcontaining ligands, such as His and Cys.…”

Section: Materials and Methods Cloning Of The Gene Encoding Ph1602-ementioning

confidence: 99%

Crystal structure of an RtcB homolog protein (PH1602‐extein protein) from Pyrococcus horikoshii reveals a novel fold

et al. 2006

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Section: Materials and Methods Cloning Of The Gene Encoding Ph1602-ementioning

confidence: 99%

Crystal structure of an RtcB homolog protein (PH1602‐extein protein) from Pyrococcus horikoshii reveals a novel fold

et al. 2006

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“…Each active site contains a catalytically (46,66,120). A more recent study on the crystal structure of LuxS protein suggests that the metal ion in the native protein is Fe 2ϩ and not Zn 2ϩ (159).…”

Section: Autoinducersmentioning

confidence: 99%

Messing with Bacterial Quorum Sensing

Gonzalez

Keshavan

2006

Microbiol Mol Biol Rev

373

232

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SUMMARY Quorum sensing is widely recognized as an efficient mechanism to regulate expression of specific genes responsible for communal behavior in bacteria. Several bacterial phenotypes essential for the successful establishment of symbiotic, pathogenic, or commensal relationships with eukaryotic hosts, including motility, exopolysaccharide production, biofilm formation, and toxin production, are often regulated by quorum sensing. Interestingly, eukaryotes produce quorum-sensing-interfering (QSI) compounds that have a positive or negative influence on the bacterial signaling network. This eukaryotic interference could result in further fine-tuning of bacterial quorum sensing. Furthermore, recent work involving the synthesis of structural homologs to the various quorum-sensing signal molecules has resulted in the development of additional QSI compounds that could be used to control pathogenic bacteria. The creation of transgenic plants that express bacterial quorum-sensing genes is yet another strategy to interfere with bacterial behavior. Further investigation on the manipulation of quorum-sensing systems could provide us with powerful tools against harmful bacteria.

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“…One of these, as exemplified by that observed with Vibrio harveyi in the regulation of bioluminescence production (Bassler et al 1993;Mok et al, 2003), employs autoinducer-2 (AI-2) as the signalling molecule. The V. harveyi AI-2 is produced from S-adenosylhomocysteine (SAH) via enzymic steps involving the nucleosidase Pfs, which converts SAH to S-ribosylhomocysteine (SRH), and LuxS, which converts SRH to 4,5-dihydroxy-2,3-pentanedione, the immediate precursor of AI-2 (De Keersmaecker et al, 2006;Lewis et al, 2001;Schauder et al, 2001). Unlike AI-1, which is generally species-specific and is responded to only by cells of kindred genetic lineages, AI-2 has been discovered to exist in diverse bacteria and the AI-2 molecules produced by one bacterial species are responded to by bacteria of different species and genera.…”

Section: Introductionmentioning

confidence: 99%

Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain

2008

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Edwardsiella tarda is a bacterial pathogen that can infect both humans and animals. TX1, an Ed. tarda strain isolated from diseased fish, was found to produce autoinducer 2 (AI-2)-like activity that was growth phase dependent and modulated by growth conditions. The gene coding for the AI-2 synthase was cloned from TX1 and designated luxS Et. LuxS Et was able to complement the AI-2 mutant phenotype of Escherichia coli strain DH5a. Expression of luxS Et correlated with AI-2 activity and was increased by glucose and decreased by elevated temperature. The effect of glucose was shown to be mediated through the cAMP-CRP complex, which repressed luxS Et expression. Overexpression of luxS Et enhanced AI-2 activity in TX1, whereas disruption of luxS Et expression by antisense RNA interference (i) reduced the level of AI-2 activity, (ii) impaired bacterial growth under various conditions, (iii) weakened the expression of genes associated with the type III secretion system and biofilm formation, and (iv) attenuated bacterial virulence. Addition of exogenous AI-2 was able to complement the deficiencies in the expression of TTSS genes and biofilm production but failed to rescue the growth defects. Our results (i) demonstrated that the AI-2 activity in TX1 is controlled at least in part at the level of luxS Et expression, which in turn is regulated by growth conditions, and that the temporal expression of luxS Et is essential for optimal bacterial infection and survival; and (ii) suggested the existence in Ed. tarda of a LuxS/AI-2-mediated signal transduction pathway that regulates the production of virulence-associated elements.

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A Structural Genomics Approach to the Study of Quorum Sensing

Cited by 88 publications

References 22 publications

Crystal structure of an RtcB homolog protein (PH1602‐extein protein) from Pyrococcus horikoshii reveals a novel fold

Crystal structure of an RtcB homolog protein (PH1602‐extein protein) from Pyrococcus horikoshii reveals a novel fold

Messing with Bacterial Quorum Sensing

Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain

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