A Stress-Responsive Gene from Groundnut, Gdi-15, Is Homologous to Flavonol 3-O-Glucosyltransferase Involved in Anthocyanin Biosynthesis

Ramaswamy, Gopalakrishna; Kumar, Ganesh; Krishnaprasad, B. T.; Mathew, M. K.; Kumar, Manish

doi:10.1006/bbrc.2001.4992

Cited by 31 publications

(24 citation statements)

References 25 publications

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“…Zeng et al (2002) isolated two early light-induced proteins from resurrection plant (Tortula ruralis) in response to diverse environmental conditions and found these genes play a key role in the protection and repair of photosynthetic apparatus, and also known to protect against photo oxidative stress (Hutin et al 2003). Gopalakrishna et al (2001) noticed that the expression level of early lightinduced protein was the maximum in sunflower at 30% soil moisture level. In the present study, we observed a gradual increase in the expression of early light-induced protein with increasing stress intensity (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Purification of poly (A ? ) RNA was carried out separately from both control and stressed RNA by magnetic separation after annealing to 5 0 -biotinylated oligo-dT primer as described by Gopalakrishna et al (2001) and subsequently immobilizing on to streptavidin-linked paramagnetic beads as described by Mishra et al (2005).…”

Section: Rna Isolation and Mrna Purificationmentioning

confidence: 99%

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Identification of drought stress-responsive genes from drought-tolerant groundnut cultivar (Arachis hypogaea L. cv K-134) through analysis of subtracted expressed sequence tags

et al. 2011

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Drought is one of the major abiotic stresses limiting crop productivity in arid and semi-arid regions and influences many aspects of plant development. Groundnut (Arachis hypogaea L.) is an important oil yielding crop and considered as relatively drought tolerant. In this study, two groundnut cultivars were first tested for their drought tolerance based on physiological marker attributes such as relative water content, total chlorophyll content, cell membrane stability and free proline content and identified cultivar K-134 as a drought tolerant and cultivar JL-24 as drought susceptible. To gain a better understanding of the drought stress responses at molecular level, we carried out a genomic analysis of stress-responsive genes/transcripts in drought-tolerant cultivar K-134. As a first step toward characterization of stress-responsive genes, construction and analysis of subtracted cDNA library from droughttolerant cultivar (K-134) is reported here. Using this strategy a total of 200 ESTs were isolated, sequenced, of which 120 high-quality ESTs were obtained and clustered. Further, our analysis revealed that 31% sequences were unique and no homology to known proteins in the database. This observation has great relevance since groundnut is a stress-adapted legume crop. Further, to validate the identified differentially expressed genes, expression profiles of selected clones were analyzed using dot blot (reverse northern), northern blot analysis. We showed that these clones are differentially expressed under different abiotic stress conditions. The implications of the analyzed genes in abiotic stress tolerance were discussed.

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Section: Resultsmentioning

confidence: 99%

Section: Rna Isolation and Mrna Purificationmentioning

confidence: 99%

Identification of drought stress-responsive genes from drought-tolerant groundnut cultivar (Arachis hypogaea L. cv K-134) through analysis of subtracted expressed sequence tags

et al. 2011

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“…In one of our earlier studies it has been shown that the transcript levels of F3OGT (NCBI GenBank Accession no. DQ256368 and AY029322) significantly increased under stress (Gopalakrishna et al 2001). However, marginal increase in anthocyanin was seen under water deficit stress during this study.…”

Section: Screening Silenced Plants and Functional Characterizationmentioning

confidence: 90%

“…Amongst them F3OGT silenced plants showed more susceptible phenotype. Earlier the F3OGT had been shown to be up-regulated under drought in peanut (Gopalakrishna et al 2001). Also this gene is involved in regulating anthocyanin levels which is known to play role in drought tolerance by protecting membrane lipids and can also act as antioxidants.…”

Section: Chlorophyll Degradation In Silenced Plants Under Water Deficmentioning

confidence: 99%

Functional characterization of Nicotiana benthamiana homologs of peanut water deficit-induced genes by virus-induced gene silencing

Senthil‐Kumar

Govind

et al. 2006

Planta

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Determining the functional role of genes that are differentially regulated during a stress response is challenging. In this study, few water deficit-induced genes from peanut were characterized in Nicotiana benthamiana using virus-induced gene silencing (VIGS) and their relevance for stress adaptation was validated. Twenty-five cDNA clones from peanut water deficit stress-induced cDNA library that had more than 50% nucleotide similarity with N. benthamiana or tomato homologs were selected. VIGS in peanut is not yet feasible and therefore we characterized these 25 genes in N. benthamiana. Increased membrane damage was seen under water deficit stress in most of the silenced plants signifying that many of these stress-induced genes are important to confer drought tolerance. Among the genes tested, silencing by homolog of flavonol 3-O-glucosyltransferase (F3OGT), homolog of alcohol dehydrogenase, homologous to salt inducible protein, and homolog of heat shock protein 70 showed more visible wilting symptoms compared with the control plants during water deficit stress. Interestingly, down-regulation of two genes, homologous to aspartic proteinase 2, and homolog of Jumonji class of transcription factor showed relative drought tolerant phenotypes. F3OGT silenced plants showed more wilting symptoms, membrane damage and chlorophyll degradation than any other silenced plants during water deficit. Our results demonstrate that VIGS approach can be used to characterize and assess the functional relevance of water deficit stress-induced cDNAs in a heterologous species.

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“…Several studies have shown that GTases are induced by a variety of stresses, including cold, pathogens (Bowles 1998;Lee and Raskin 1999), desiccation (Gopalakrishna et al 2001), SA (Horvath and Chua 1996) and methyl jasmonate (Imanishi et al 1998). To determine whether the expression levels of UGTCs2 and UGTCs3 were influenced by exogenous hormones, or by other stimuli such as drought stress and wounding, total RNA was isolated from treated leaves and used as a template in the RT-PCR reactions.…”

Section: Unaltered Expression Of the Gtase Genes Under Stress Conditionsmentioning

confidence: 99%

Glucosylation of the saffron apocarotenoid crocetin by a glucosyltransferase isolated from Crocus sativus stigmas

Rubio-Moraga¹,

Nohales²,

Pérez³

et al. 2004

Planta

132

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Saffron, the dry stigma of Crocus sativus L., is considered to be the world's most expensive spice. Three major apocarotenoids--crocin, crocetin and picrocrocin--are responsible for the colour and bitter taste of saffron. The final step in the biosynthesis of the 20-carbon esterified carotenoid crocin is the transformation of the insoluble crocetin into a soluble and stable storage form by glucosylation. These glucosylation reactions are catalysed by glucosyltransferases (GTases) that play a crucial role in natural-product biosynthesis. Using degenerate primers designed to match the plant secondary product GTase (PSPG) box we cloned two cDNAs, UGTCs2 and UGTCs3, from C. sativus stigmas that encode putative polypeptides of 460 and 475 amino acids, respectively. These genes were expressed differentially in saffron tissues. UGTCs2 was mainly expressed in fully developed stigmas, whereas UGTCs3 was mainly expressed in stamens. The UGTCs2 transcript was not detected in the stigma tissue of a Crocus species that does not synthesize crocin, while UGTCs3 and other structural genes for carotenoid biosynthesis were expressed in the stigma of all tested Crocus species. To identify the biochemical function of UGTCs2, the isolated cDNA was expressed in Escherichia coli cells. The recombinant protein UGTCs2 had glucosylation activity against crocetin, crocetin beta-D-glucosyl ester and crocetin beta-D-gentibiosyl ester. These results might suggest that the isolated clone UGTCs2 codes for a saffron crocetin GTase.

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A Stress-Responsive Gene from Groundnut, Gdi-15, Is Homologous to Flavonol 3-O-Glucosyltransferase Involved in Anthocyanin Biosynthesis

Cited by 31 publications

References 25 publications

Identification of drought stress-responsive genes from drought-tolerant groundnut cultivar (Arachis hypogaea L. cv K-134) through analysis of subtracted expressed sequence tags

Identification of drought stress-responsive genes from drought-tolerant groundnut cultivar (Arachis hypogaea L. cv K-134) through analysis of subtracted expressed sequence tags

Functional characterization of Nicotiana benthamiana homologs of peanut water deficit-induced genes by virus-induced gene silencing

Glucosylation of the saffron apocarotenoid crocetin by a glucosyltransferase isolated from Crocus sativus stigmas

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