A stress associated protein from Chinese wild Vitis amurensis, VaSAP15, enhances the cold tolerance of transgenic grapes

Shu, Xin; Lei, Ding; Gu, Bao; Zhang, Hongjuan; Guan, Pingyin; Zhang, Jianxia

doi:10.1016/j.scienta.2021.110147

Cited by 14 publications

(6 citation statements)

References 82 publications

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“…Most functional analyses of SAPs in plants have focused on typical SAP proteins, which have one AN1 domain and one A20 domain, while only a few studies have investigated SAPs containing only AN1 domains [ 22 , 39 , 44 , 45 ] and AN1-AN1-C 2 H 2 -C 2 H 2 type SAPs [ 11 ]. IbSAP16 is one of the two AN1-AN1-C 2 H 2 -C 2 H 2 encoding genes in sweetpotato.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of the A20/AN1 Zinc Finger Protein Family Genes in Ipomoea batatas and Its Two Relatives and Function Analysis of IbSAP16 in Salinity Tolerance

Xie

Yang

Wang

et al. 2022

IJMS

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Stress-associated protein (SAP) genes—encoding A20/AN1 zinc-finger domain-containing proteins—play pivotal roles in regulating stress responses, growth, and development in plants. They are considered suitable candidates to improve abiotic stress tolerance in plants. However, the SAP gene family in sweetpotato (Ipomoea batatas) and its relatives is yet to be investigated. In this study, 20 SAPs in sweetpotato, and 23 and 26 SAPs in its wild diploid relatives Ipomoea triloba and Ipomoea trifida were identified. The chromosome locations, gene structures, protein physiological properties, conserved domains, and phylogenetic relationships of these SAPs were analyzed systematically. Binding motif analysis of IbSAPs indicated that hormone and stress responsive cis-acting elements were distributed in their promoters. RT-qPCR or RNA-seq data revealed that the expression patterns of IbSAP, ItbSAP, and ItfSAP genes varied in different organs and responded to salinity, drought, or ABA (abscisic acid) treatments differently. Moreover, we found that IbSAP16 driven by the 35 S promoter conferred salinity tolerance in transgenic Arabidopsis. These results provided a genome-wide characterization of SAP genes in sweetpotato and its two relatives and suggested that IbSAP16 is involved in salinity stress responses. Our research laid the groundwork for studying SAP-mediated stress response mechanisms in sweetpotato.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of the A20/AN1 Zinc Finger Protein Family Genes in Ipomoea batatas and Its Two Relatives and Function Analysis of IbSAP16 in Salinity Tolerance

Xie

Yang

Wang

et al. 2022

IJMS

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“…Гены, кодирующие семейство стресс-ассоциированных белков (Stress-Associated Proteins, SAP), могут реагировать на различные биотические и абиотические стрессовые факторы, и играют важную роль в процессах повышения устойчивости растений к различным стрессовым воздействиям. Shu с соавторами (Shu et al, 2021) изучали защитную функцию гена VaSAP15 в контроле устойчивости к низким температурам у дикорастущей формы V. amurensis. Листья V. vinifera у трансгенных линий сорта ʻThompson Seedlessʼ, которые были получены методом агробактериальной трансформации и характеризова-лись сверхэкспрессией гена VaSAP15, меньше повреждались холодом по сравнению с диким типом.…”

Section: ягодные культурыunclassified

“…Уровень экспрессии некоторых генов, ассоциированных с холодоустойчивостью, включая CBF1, CBF2, CBF3, COR27, RD29B и NCED1, оказался повышенным. Устойчивость к холоду, ассоциированная с белком VaSAP15, обеспечивается взаимодействием этого белка с другими партнерами, такими как VaPDI1, белковая дисульфид-изомераза, которая модулирует пути сигнальной трансдукции и регулирует ответ генов на холодовой стресс (Shu et al, 2021).…”

Section: ягодные культурыunclassified

The new directions in genetics, breeding and biotechnology of ornamental and berry crops in the N.I. Vavilov Institute of Plant Genetic Resources (VIR)

Rakhmangulov

Barabanov

Erastenkova

et al. 2023

Biotehnologiâ i selekciâ rastenij

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The use of modern breeding methods, biotechnology, and molecular genetics makes it possible to identify promising accessions with specified economically important traits at early pre-breeding stages. The success of creating new varieties depends on the availability of unique collections of plant genetic resources, information about genomes, possibility of in vitro cultivation with high regenerative capacity, and practical skills and competencies in this area. One of the advanced methods for accelerating the breeding process is genome editing using the CRISPR/Cas system. This method allows the effective modification of genes in order to obtain varieties with desired traits. In 2022, a new youth laboratory of genetics, breeding, biotechnology of ornamental and berry crops was set up at VIR as part of the National Project "Science and Universities". It is noteworthy that this event coincided with the 135th anniversary of the birth of N.I. Vavilov. The work of the laboratory is aimed at obtaining lines with desired properties for the further breeding process; identifying target genes of economically important traits for obtaining new varieties, lines, and hybrids; as well as creating protocols for the accelerated reproduction of virus-free material of commercially demanded varieties oriented towards import substitution. This review discusses current trends in breeding of ornamental and berry crops: e.g., flower color change in snapdragon and peony; flower aroma improvement in rose; architectonics change in actinidia; and increase of resistance to stress factors in blackberries, strawberries, and grapes.

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“…VaICE1 acts upstream of CBF in the cold stress pathway, binding and activating the CBF3 promoter, which subsequently binds to the CRT/DRE cis-acting element ( CCGAC ) in the promoter region and induces the expression of the downstream cold response gene ( COR15A ) and other cold adaptation genes, thus improving frost resistance (Dong et al 2013 ). The overexpression of the VaSAP15 gene isolated from highly cold tolerant wild crape myrtle in Thompson seedless grapes improved its cold tolerance, and the damage of the leaves of the transgenic strain was significantly lower than that of the wild-type leaves after cold stress at 0 °C (Shu et al 2021 ). VvBAP1 improves grapevine cold tolerance by regulating and controlling sugar content and activating antioxidant enzyme activity.…”

Section: Research Progress Of Overwintering Measures Which Avoiding B...mentioning

confidence: 99%

Overwintering covered with soil or avoiding burial of wine grapes under cold stress: Chinese wine industry's past and future, challenges and opportunities

Wan,

Yang,

Yin

et al. 2023

Stress Biology

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In northwest China, where winter is extremely cold and the grapevine is vulnerable to freezing damage, the application of soil covering has promoted the vigorous development of the local grape and wine industries. However, in recent years, the negative effects of burying soil for cold protection on the environment have gradually emerged. In some viticultural regions, the phenomenon of "summer forest, winter desert" has appeared. Therefore, it is urgent for the Chinese grape industry to find a better solution to overwinter safely and environmentally friendly. This review summarizes the advantages and disadvantages of widely used solutions to overwinter such as covering vines with soil, breeding of cold-resistant grapes, cold-resistant cultivation model, physical and chemical covering materials, and protected grape facilities were reviewed. Future overwintering measures were proposed which avoid burial and grape overwintering research directions. It also provides a theoretical foundation and technical support to improve grape yield and quality in northwest China.

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A stress associated protein from Chinese wild Vitis amurensis, VaSAP15, enhances the cold tolerance of transgenic grapes

Cited by 14 publications

References 82 publications

Genome-Wide Identification of the A20/AN1 Zinc Finger Protein Family Genes in Ipomoea batatas and Its Two Relatives and Function Analysis of IbSAP16 in Salinity Tolerance

Genome-Wide Identification of the A20/AN1 Zinc Finger Protein Family Genes in Ipomoea batatas and Its Two Relatives and Function Analysis of IbSAP16 in Salinity Tolerance

The new directions in genetics, breeding and biotechnology of ornamental and berry crops in the N.I. Vavilov Institute of Plant Genetic Resources (VIR)

Overwintering covered with soil or avoiding burial of wine grapes under cold stress: Chinese wine industry's past and future, challenges and opportunities

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