A streamlined method to generate endothelial cells from human pluripotent stem cells via transient doxycycline-inducible ETV2 activation

Luo, Allen Chilun; Wang, Jiuhai; Wang, Kai; Zhu, Yonglin; Gong, Liyan; Lee, Umji; Li, Xiang; Tremmel, Daniel M.; Lin, Ruei-Zeng; Ingber, Donald E.; Gorman, James; Melero-Martin, Juan M.

doi:10.1007/s10456-024-09937-5

Angiogenesis

2024

DOI: 10.1007/s10456-024-09937-5

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A streamlined method to generate endothelial cells from human pluripotent stem cells via transient doxycycline-inducible ETV2 activation

Allen Chilun Luo,

Jiuhai Wang,

Kai Wang

et al.

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2024

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Cited by 1 publication

References 52 publications

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miR126-mediated impaired vascular integrity in Rett syndrome

Osaki,

Wan,

Haratani

et al. 2024

Preprint

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Rett syndrome (RTT) is a neurodevelopmental disorder that is caused by mutations in melty-CpG binding protein 2 (MeCP2). MeCP2 is a non-cell type-specific DNA binding protein, and its mutation influences not only neural cells but also non-neural cells in the brain, including vasculature associated with endothelial cells. Vascular integrity is crucial for maintaining brain homeostasis, and its alteration may be linked to the pathology of neurodegenerative disease, but a non-neurogenic effect, especially the relationship between vascular alternation and Rett syndrome pathogenesis, has not been shown. Here, we recapitulate a microvascular network using Rett syndrome patient-derived induced pluripotent stem (iPS) cells that carry MeCP2[R306C] mutation to investigate early developmental vascular impact. To expedite endothelial cell differentiation, doxycycline (DOX)-inducible ETV2 expression vectors were inserted into the AAVS1 locus of Rett syndrome patient-derived iPS cells and its isogenic control by CRISPR/Cas9. With these endothelial cells, we established a disease microvascular network (Rett-dMVNs) and observed higher permeability in the Rett-dMVNs compared to isogenic controls, indicating altered barrier function by MeCP2 mutation. Furthermore, we unveiled that hyperpermeability is involved in the upregulation of miR126-3p in Rett syndrome patient-derived endothelial cells by microRNA profiling and RNAseq, and rescue of miR126-3p level can recover their phenotype. We discover miR126-3p-mediated vascular impairment in Rett syndrome patients and suggest the potential application of these findings for translational medicine.

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miR126-mediated impaired vascular integrity in Rett syndrome

Osaki,

Wan,

Haratani

et al. 2024

Preprint

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show abstract

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A streamlined method to generate endothelial cells from human pluripotent stem cells via transient doxycycline-inducible ETV2 activation

Cited by 1 publication

References 52 publications

miR126-mediated impaired vascular integrity in Rett syndrome

miR126-mediated impaired vascular integrity in Rett syndrome

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