A strategy for screening bioactive components from natural products based on two-dimensional cell membrane chromatography and component-knockout approach

Liu, Run‐Zhou; Rui, Wen; An, Hai-Ming; Liu, Xinguang; Li, Chaoran; Li, Ping; Yang, Hua

doi:10.1016/j.chroma.2019.04.066

Cited by 15 publications

(5 citation statements)

References 32 publications

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“…The development of novel functional foods and nutraceuticals has been increasing largely because, on one hand, the market is demanding these products, and on the other hand, the chemistry and biochemistry of natural products as well as food technology and biotechnology have allowed important advancements. Moreover, countless studies undertaken to confirm the health claims of this type of product or their bioactive components have contributed for a great development of this market [57,[81][82][83][84][85][86].…”

Section: Plant-based Dairy Alternativesmentioning

confidence: 99%

The Link between the Consumer and the Innovations in Food Product Development

Guiné

Florença

Barroca

et al. 2020

Foods

146

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New lifestyles, higher incomes and better consumer awareness are increasing the demand for a year-round supply of innovative food products. In past decades, important developments have been achieved in areas related to food and the food industry. This review shows that factors influencing performance in new product development (NPD) are dynamic and continuously guiding project development. The data obtained by direct involvement of consumers can impact positively successful product development and enhance the company’s financial performance. The study of consumer behaviour and attitudes towards new foods encompasses multiple aspects, such as preference, choice, desire to eat certain foods, buying intentions and frequency of consumption. Additionally, both the consumers’ willingness to purchase and the willingness to pay a premium are important in NPD, launching and success.

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Section: Plant-based Dairy Alternativesmentioning

confidence: 99%

The Link between the Consumer and the Innovations in Food Product Development

Guiné

Florença

Barroca

et al. 2020

Foods

146

View full text Add to dashboard Cite

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“…Liu et al established a 2D H9c2/CMC-HPLC coupled with a QTOF-MS system to screen seven myocardial protection compounds from red yeast rice. 84 In the 1 D separation, three fractions were first screened, and four additional bioactive compounds were identified after knocking out high content/affinity compounds. Notably, the two rounds of screening avoided column overload caused by high concentration and promoted the enrichment of trace compounds, providing another possibility for discovery of trace compounds from complex matrices.…”

Section: Zonal Elution-based 2d Modementioning

confidence: 99%

“…Liu et al . established a 2D H9c2/CMC‐HPLC coupled with a QTOF‐MS system to screen seven myocardial protection compounds from red yeast rice 84 . In the 1 D separation, three fractions were first screened, and four additional bioactive compounds were identified after knocking out high content/affinity compounds.…”

Section: Application Of 2d Chromatography In Screening Of Bioactive C...mentioning

confidence: 99%

Two‐dimensional chromatography in screening of bioactive components from natural products

Xiang

Qiu

et al. 2022

Phytochemical Analysis

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Introduction: Screening and analysis of bioactive components from natural products is a fundamental part of new drug development and innovation. Two-dimensional (2D) chromatography has been demonstrated to be an effective method for screening and preparation of specific bioactive components from complex natural products. Objective: To collect details of application of 2D chromatography in screening of natural product bioactive components and to outline the research progress of different separation mechanisms and strategies. Methodology: Three screening strategies based on 2D chromatography are reviewed, including traditional separation-based screening, bioactivity-guided screening and affinity chromatography-based screening. Meanwhile, in order to cover these aspects, selections of different separation mechanisms and modes are also presented.Results: Compared with traditional one-dimensional (1D) chromatography, 2D chromatography has unique advantages in terms of peak capacity and resolution, and it is more effective for screening and identifying bioactive components of complex natural products. Conclusion:Screening of natural bioactive components using 2D chromatography helps separation and analysis of complex samples with greater targeting and relevance, which is very important for development of innovative drug leads.

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“…The classical methods for screening involve the separation and purification of a single compound from complex TCM extracts, followed by biological validation of each purified compound in sequence. In recent years, several novel methods for screening drugs have emerged, such as two‐dimensional cell membrane chromatography (Liu et al, ) and cell membrane cloaked magnetic carbon nanotubes, to screen bioactive lead compounds from TCMs (Hu, Bu, Cao, et al, , Hu, Bu, Zhen, et al, ). These methods are less time‐consuming and have satisfactory selectivity.…”

Section: Introductionmentioning

confidence: 99%

A rapid assay to screen adenosine deaminase inhibitors from Ligustri Lucidi Fructus against metabolism of cordycepin utilizing ultra‐high‐performance liquid chromatography–tandem mass spectrometry

Guan

Liu

et al. 2020

Biomedical Chromatography

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Cordycepin has recently received increased attention owing to its extensive pharmacological activity. Adenosine deaminase (ADA) is widely distributed in mammalian blood and tissues; as a result, cordycepin is quickly metabolized upon entering into the body and converted into the inactive metabolite 3′‐deoxyinosine, thus limiting its activity when administered alone. We herein present a novel ultra‐high‐performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method for screening ADA inhibitors against the metabolism of cordycepin. Cordycepin and 3′‐deoxyinosine were chosen as substrate and product, respectively. A proper separation was achieved for all analytes within 3 min. 3′‐Deoxyinosine was quantified in the presence or absence of potential ADA inhibitors to evaluate ADA activity. The assay can simultaneously determine substrate and product, with the endogenous substance and ADA inhibitors added not interfering in its activity. After optimizing the enzymatic incubation and UHPLC–MS/MS conditions, Km and Vmax values for ADA deamination of cordycepin were 95.18 ± 7.85 μm and 363.90 ± 12.16 μmol/min/unit, respectively. Oleanolic acid and ursolic acid from Ligustri Lucidi Fructus were chosen as ADA inhibitors with half maximal inhibitory concentration values of 21.82 ± 0.39 and 18.41 ± 0.14 μm, respectively. A non‐competitive inhibition model was constructed and this assay can be used to screen other potential ADA inhibitors quickly and accurately.

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A strategy for screening bioactive components from natural products based on two-dimensional cell membrane chromatography and component-knockout approach

Cited by 15 publications

References 32 publications

The Link between the Consumer and the Innovations in Food Product Development

The Link between the Consumer and the Innovations in Food Product Development

Two‐dimensional chromatography in screening of bioactive components from natural products

A rapid assay to screen adenosine deaminase inhibitors from Ligustri Lucidi Fructus against metabolism of cordycepin utilizing ultra‐high‐performance liquid chromatography–tandem mass spectrometry

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