The stabilization mechanism of water-in-oil (W/O) emulsions
has
been studied by measuring the interactions between two water droplets
in n-tetradecane using atomic force microscopy. The
effects of water-soluble surfactants (SDS/CTAB/Tween 80), an oil-soluble
surfactant (Span 20), and the coexistence of the water and oil-soluble
surfactants on the stability of water droplets in oil were investigated
separately. It is found that the addition of oil-soluble surfactants
(Span 20) prevents the coalescence of water droplets in oil. To discuss
the role of an oil-soluble surfactant, we analyzed the force curve
by applying the theoretical model. The results demonstrate that the
oil-soluble surfactant (Span 20) stabilizes dispersed droplets by
adsorbing onto the interface and forming a relatively tighter layer
with the increase in surfactant concentration, which hinders film
rupture. This behavior of the surfactant could also be properly characterized
by steric hindrance. A further step was taken by introducing another
water-soluble surfactant. It is found that the addition of either
SDS or CTAB into the water phase is futile in inducing droplet coalescence
in the presence of Span 20. In contrast, Tween 80 was found to be
effective in destabilizing water droplets, which could be due to the
competitive adsorption between Tween 80 and Span 20 at the interface.
By characterizing the interfacial adsorption of Tween 80 and Span
20 with a theoretical adsorption isotherm model, the result indicates
that interface replacement would result in a loose adsorption layer
that is insufficient to hinder droplet coalescence. Our study provides
an intriguing understanding of the role of surfactants in the stabilization
and destabilization of water-in-oil emulsions.