A strategy for building an amplified transcriptional switch to detect bacterial contamination of plants

Czarnecka, Eva; Verner, F. Lance; Gurley, William B.

doi:10.1007/s11103-011-9845-2

Cited by 11 publications

(7 citation statements)

References 42 publications

(63 reference statements)

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“…The promoter controls genes activity which suppresses chlorophyll synthesis and induces its degradation. Lettuce and Arabidopsis have been modified to signal for bacterial contamination effectively through a gene circuit controlled by a positive autoregulatory transcriptional feedback loop (Czarnecka et al, 2012). This emerging approach has great potential to be used broadly, for developing sentinel plant that detects abiotic stresses including high temperature in field and activates gene regulation machinery for imparting heat stress tolerance.…”

Section: Role Of Synthetic Biology For Developing Resilient Crops Against High Temperature Stressmentioning

confidence: 99%

Response and tolerance mechanism of food crops under high temperature stress: a review

et al. 2022

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High temperature stress events are critical factors inhibiting crop yield. Meanwhile, world population is growing very rapidly and will be reached up to 9 billion by 2050. To feed increasing world population, it is challenging task to increase about 70% global food productions. Food crops have significant contribution toward global food demand and food security. However, consequences from increasing heat stress events are demolishing their abilities to survive and sustain yield when subjected to extreme high temperature stress. Therefore, there is dire need to better understand response and tolerance mechanism of food crops following exposure to heat stress. Here, we aimed to provide recent update on impact of high temperature stress on crop yield of food crops, pollination, pollinators, and novel strategies for improving tolerance of food crop under high temperature stress. Importantly, development of heat-resistant transgenic food crops can grant food security through transformation of superior genes into current germplasm, which are associated with various signaling pathways as well as epigenetic regulation in response to extreme high temperature stress.

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Section: Role Of Synthetic Biology For Developing Resilient Crops Against High Temperature Stressmentioning

confidence: 99%

Response and tolerance mechanism of food crops under high temperature stress: a review

et al. 2022

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“…The promoter controls the activity of genes which inhibit chlorophyll synthesis and initiates its degradation. Arabidopsis and lettuce protoplasts were modified to signal for bacterial contamination efficiently via a gene circuit controlled by a positive autoregulatory transcriptional feedback loop (Czarnecka et al, 2012). This emerging technology has the potential to be applied broadly, with the development of sentinel plants that detect specific or multiple abiotic stresses such as heat, salinity, and drought in the field and initiates a gene regulatory network for imparting stress tolerance.…”

Section: Synthetic Biology As An Emerging Approachmentioning

confidence: 99%

Engineering Multiple Abiotic Stress Tolerance in Canola, Brassica napus

et al. 2020

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Impacts of climate change like global warming, drought, flooding, and other extreme events are posing severe challenges to global crop production. Contribution of Brassica napus towards the oilseed industry makes it an essential component of international trade and agroeconomics. Consequences from increasing occurrences of multiple abiotic stresses on this crop are leading to agroeconomic losses making it vital to endow B. napus crop with an ability to survive and maintain yield when faced with simultaneous exposure to multiple abiotic stresses. For an improved understanding of the stress sensing machinery, there is a need for analyzing regulatory pathways of multiple stressresponsive genes and other regulatory elements such as non-coding RNAs. However, our understanding of these pathways and their interactions in B. napus is far from complete. This review outlines the current knowledge of stress-responsive genes and their role in imparting multiple stress tolerance in B. napus. Analysis of network cross-talk through omics data mining is now making it possible to unravel the underlying complexity required for stress sensing and signaling in plants. Novel biotechnological approaches such as transgene-free genome editing and utilization of nanoparticles as gene delivery tools are also discussed. These can contribute to providing solutions for developing climate change resilient B. napus varieties with reduced regulatory limitations. The potential ability of synthetic biology to engineer and modify networks through fine-tuning of stress regulatory elements for plant responses to stress adaption is also highlighted.

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“…This result is useful for applications such as sensing of external signals, where a small or transient exposure to a compound leads to amplification of a response that is proportionally large relative to the initial signal. This was done in Arabidopsis and lettuce protoplasts ( Czarnecka et al, 2012 ), but it is not known if the system would behave similarly in stably transformed plants. Another application of synthetic positive feedback circuit is stimulus memory, in which a response to a brief exposure is maintained well beyond the time the stimulus is present.…”

Section: Plant Synthetic Biology Applicationsmentioning

confidence: 99%

Quantitative and Predictive Genetic Parts for Plant Synthetic Biology

McCarthy

Medford

2020

Front. Plant Sci.

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Plant synthetic biology aims to harness the natural abilities of plants and to turn them to new purposes. A primary goal of plant synthetic biology is to produce predictable and programmable genetic circuits from simple regulatory elements and well-characterized genetic components. The number of available DNA parts for plants is increasing, and the methods for rapid quantitative characterization are being developed, but the field of plant synthetic biology is still in its early stages. We here describe methods used to describe the quantitative properties of genetic components needed for plant synthetic biology. Once the quantitative properties and transfer function of a variety of genetic parts are known, computers can select the optimal components to assemble into functional devices, such as toggle switches and positive feedback circuits. However, while the variety of circuits and traits that can be put into plants are limitless, doing synthetic biology in plants poses unique challenges. Plants are composed of differentiated cells and tissues, each representing potentially unique regulatory or developmental contexts to introduced synthetic genetic circuits. Further, plants have evolved to be highly sensitive to environmental influences, such as light or temperature, any of which can affect the quantitative function of individual parts or whole circuits. Measuring the function of plant components within the context of a plant cell and, ideally, in a living plant, will be essential to using these components in gene circuits with predictable function. Mathematical modeling will be needed to account for the variety of contexts a genetic part will experience in different plant tissues or environments. With such understanding in hand, it may be possible to redesign plant traits to serve human and environmental needs.

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A strategy for building an amplified transcriptional switch to detect bacterial contamination of plants

Cited by 11 publications

References 42 publications

Response and tolerance mechanism of food crops under high temperature stress: a review

Response and tolerance mechanism of food crops under high temperature stress: a review

Engineering Multiple Abiotic Stress Tolerance in Canola, Brassica napus

Quantitative and Predictive Genetic Parts for Plant Synthetic Biology

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