A Straightforward Approach to Engineer Vascularized Adipose Tissue Using Microvascular Fragments

Acosta, Francisca M.; Stojkova, Katerina; Brey, Eric M.; Rathbone, Christopher R.

doi:10.1089/ten.tea.2019.0345

Cited by 15 publications

(13 citation statements)

References 29 publications

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“…It has been shown that wound healing can be promoted by encapsulating SVF cells in a collagen-fibrin hydrogel ( Nilforoushzadeh et al, 2019 ). In another study, a prevascularized adipose tissue was constructed by using MVF ( Acosta et al, 2020 ). Furthermore, a prevascularized skin substitute was developed utilizing MVF and SVF via in vivo prevascularization methods ( Später et al, 2018 ).…”

Section: Supporting Cellsmentioning

confidence: 99%

Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering

Shafiee

Shariatzadeh

Zafari

et al. 2021

Front. Bioeng. Biotechnol.

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Currently, the fabrication of a functional vascular network to maintain the viability of engineered tissues is a major bottleneck in the way of developing a more advanced engineered construct. Inspired by vasculogenesis during the embryonic period, the in vitro prevascularization strategies have focused on optimizing communications and interactions of cells, biomaterial and culture conditions to develop a capillary-like network to tackle the aforementioned issue. Many of these studies employ a combination of endothelial lineage cells and supporting cells such as mesenchymal stem cells, fibroblasts, and perivascular cells to create a lumenized endothelial network. These supporting cells are necessary for the stabilization of the newly developed endothelial network. Moreover, to optimize endothelial network development without impairing biomechanical properties of scaffolds or differentiation of target tissue cells, several other factors, including target tissue, endothelial cell origins, the choice of supporting cell, culture condition, incorporated pro-angiogenic factors, and choice of biomaterial must be taken into account. The prevascularization method can also influence the endothelial lineage cell/supporting cell co-culture system to vascularize the bioengineered constructs. This review aims to investigate the recent advances on standard cells used in in vitro prevascularization methods, their co-culture systems, and conditions in which they form an organized and functional vascular network.

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Section: Supporting Cellsmentioning

confidence: 99%

Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering

Shafiee

Shariatzadeh

Zafari

et al. 2021

Front. Bioeng. Biotechnol.

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“…Adipose tissue-derived MVFs consist of small segments of arterioles, venules, and capillaries (Laschke et al, 2021) and have been explored as an avenue for enhancing vascularization in engineered tissues (Laschke and Menger, 2015;Frueh et al, 2017a;Acosta et al, 2020). Although MVFs do contain the fundamental cells of microvasculature, endothelial cells, and pericytes, they also contain a variety of perivascular cells that influence tissue formation (Mcdaniel et al, 2014;Acosta et al, 2020). Specifically, isolated MVFs contain macrophages (23.6%), T-cells (0.8%), progenitor cells (11.4%), myeloid cells (5.1%), hematopoietic cells (26.7%), pericytes (28.2%), and endothelial cells (21%) (Advances Solutions, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, we and others have demonstrated that stem cells with adipogenic potential are resident within MVFs after isolation (Laschke and Menger, 2015;Acosta et al, 2020). However, it was unknown if MVFs could be used as a source for generating adipose microtissues with thermogenic characteristics.…”

Section: Introductionmentioning

confidence: 99%

Engineering Human Beige Adipose Tissue

Porras

Stojkova

Acosta

et al. 2022

Front. Bioeng. Biotechnol.

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In this study, we described a method for generating functional, beige (thermogenic) adipose microtissues from human microvascular fragments (MVFs). The MVFs were isolated from adipose tissue acquired from adults over 50 years of age. The tissues express thermogenic gene markers and reproduce functions essential for the potential therapeutic impact of beige adipose tissues such as enhanced lipid metabolism and increased mitochondrial respiration. MVFs serve as a potential single, autologous source of cells that can be isolated from adult patients, induced to recreate functional aspects of beige adipose tissue and enable rapid vascularization post-transplantation. This approach has the potential to be used as an autologous therapy for metabolic diseases or as a model for the development of a personalized approach to high-throughput drug development/screening for adipose tissue.

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“…44,45 Previous work from our group and others has demonstrated that in addition to endothelial cell-lined capillaries, MVF are a source of adipose derived stem and progenitor cells, mural cells, and immune cells. 31,32,37 While it is established that MVF can be used to generate white adipose tissue, 31,32,37,46 it was unknown if MVF could be used to generate beige adipose tissue, and to what extent diabetes affects this process.…”

Section: Discussionmentioning

confidence: 99%

“…Importantly, MVF also contain perivascular stem cells with multi-differentiation potential, including adipogenic differentiation. 31,32 Collectively, MVF are a unique tool to exploit the relationship between vascularization and adipogenesis and provide an autologous source of both vessels and adipocytes. In this work, vascularized beige and white adipose tissue models were developed with MVF derived from both healthy and T2D animals.…”

Section: Introductionmentioning

confidence: 99%

Engineering Functional Vascularized Beige Adipose Tissue from Microvascular Fragments of Models of Healthy and Type II Diabetes Conditions

et al. 2022

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Engineered beige adipose tissues could be used for screening therapeutic strategies or as a direct treatment for obesity and metabolic disease. Microvascular fragments are vessel structures that can be directly isolated from adipose tissue and may contain cells capable of differentiation into thermogenic, or beige, adipocytes. In this study, culture conditions were investigated to engineer three-dimensional, vascularized functional beige adipose tissue using microvascular fragments isolated from both healthy animals and a model of type II diabetes (T2D). Vascularized beige adipose tissues were engineered and exhibited increased expression of beige adipose markers, enhanced function, and improved cellular respiration. While microvascular fragments isolated from both lean and diabetic models were able to generate functional tissues, differences were observed in regard to vessel assembly and tissue function. This study introduces an approach that could be employed to engineer vascularized beige adipose tissues from a single, potentially autologous source of cells.

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A Straightforward Approach to Engineer Vascularized Adipose Tissue Using Microvascular Fragments

Cited by 15 publications

References 29 publications

Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering

Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering

Engineering Human Beige Adipose Tissue

Engineering Functional Vascularized Beige Adipose Tissue from Microvascular Fragments of Models of Healthy and Type II Diabetes Conditions

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