A step change in the transfer of interspecific variation into wheat from <i>Amblyopyrum muticum</i>

King, Julie; Grewal, Surbhi; Yang, Caiyun; Hubbart, Stella; Scholefield, Duncan; Ashling, Stephen; Hubbart-Edwards, Stella; Allen, Alexandra M.; Burridge, Amanda J.; Bloor, Claire; Davassi, Alessandro; Silva, Glacy Jaqueline da; Chalmers, K. J.; King, I. P.

doi:10.1111/pbi.12606

Cited by 105 publications

(168 citation statements)

References 42 publications

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“…However, only limited use of this enormous source of genetic variation has been applied to strategic plant breeding programmes (King et al ., ). A major block to the large‐scale, genome‐wide application of genetic variation from wild relatives has been the lack of high‐throughput screening technology to quickly identify and characterize introgressions (King et al ., ). The alien chromosomes in disomic addition lines can be identified by morphological analysis, chromosome banding, in situ hybridization and molecular markers (Hu et al ., ; Kishii et al ., ; McArthur et al ., ; Schneider et al ., ; Wang et al ., ; Wu et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Moreover, 218 genome‐wide wheat/ Ambylopyrum muticum introgressions were detected and characterized using these SNP markers, and seven linkage groups of Am. muticum were constructed to determine the syntenic relationships between the wild relative and hexaploid wheat (King et al ., ). Therefore, the array developed using these SNPs can be employed to characterize wheat‐related species and would provide the wheat community with a valuable resource for the characterization and breeding of hexaploid and tetraploid wheat.…”

Section: Introductionmentioning

confidence: 97%

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Construction of Agropyron Gaertn. genetic linkage maps using a wheat 660K SNP array reveals a homoeologous relationship with the wheat genome

Zhou

Zhang

Che

et al. 2017

Plant Biotechnology Journal

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Summary Agropyron Gaertn. (P genome) is a wild relative of wheat that harbours many genetic variations that could be used to increase the genetic diversity of wheat. To agronomically transfer important genes from the P genome to a wheat chromosome by induced homoeologous pairing and recombination, it is necessary to determine the chromosomal relationships between Agropyron and wheat. Here, we report using the wheat 660K single nucleotide polymorphism (SNP) array to genotype a segregating Agropyron F1 population derived from an interspecific cross between two cross‐pollinated diploid collections ‘Z1842’ [A. cristatum (L.) Beauv.] (male parent) and ‘Z2098’ [A. mongolicum Keng] (female parent) and 35 wheat–A. cristatum addition/substitution lines. Genetic linkage maps were constructed using 913 SNP markers distributed among seven linkage groups spanning 839.7 cM. The average distance between adjacent markers was 1.8 cM. The maps identified the homoeologous relationship between the P genome and wheat and revealed that the P and wheat genomes are collinear and relatively conserved. In addition, obvious rearrangements and introgression spread were observed throughout the P genome compared with the wheat genome. Combined with genotyping data, the complete set of wheat–A. cristatum addition/substitution lines was characterized according to their homoeologous relationships. In this study, the homoeologous relationship between the P genome and wheat was identified using genetic linkage maps, and the detection mean for wheat–A. cristatum introgressions might significantly accelerate the introgression of genetic variation from Agropyron into wheat for exploitation in wheat improvement programmes.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Construction of Agropyron Gaertn. genetic linkage maps using a wheat 660K SNP array reveals a homoeologous relationship with the wheat genome

Zhou

Zhang

Che

et al. 2017

Plant Biotechnology Journal

View full text Add to dashboard Cite

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“…Similar efforts have been made to introduce variation from both evolutionarily near and distant wild wheat relatives (King et al ., ), although these strategies require specialized genetic stocks to allow the transfer of wild wheat chromosome segments. These segments do not freely recombine, therefore, in addition to the beneficial gene, large DNA fragments from the wild relative are introduced often with negative consequences (linkage drag).…”

Section: Use Of Natural Variation For Trait Discoverymentioning

confidence: 99%

Applying the latest advances in genomics and phenomics for trait discovery in polyploid wheat

2018

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Summary Improving traits in wheat has historically been challenging due to its large and polyploid genome, limited genetic diversity and in‐field phenotyping constraints. However, within recent years many of these barriers have been lowered. The availability of a chromosome‐level assembly of the wheat genome now facilitates a step‐change in wheat genetics and provides a common platform for resources, including variation data, gene expression data and genetic markers. The development of sequenced mutant populations and gene‐editing techniques now enables the rapid assessment of gene function in wheat directly. The ability to alter gene function in a targeted manner will unmask the effects of homoeolog redundancy and allow the hidden potential of this polyploid genome to be discovered. New techniques to identify and exploit the genetic diversity within wheat wild relatives now enable wheat breeders to take advantage of these additional sources of variation to address challenges facing food production. Finally, advances in phenomics have unlocked rapid screening of populations for many traits of interest both in greenhouses and in the field. Looking forwards, integrating diverse data types, including genomic, epigenetic and phenomics data, will take advantage of big data approaches including machine learning to understand trait biology in wheat in unprecedented detail.

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“…A recent successful example of this initially un-focused generation of genetic diversity accompanied by high-throughput genotyping involves wheat and one of its wild relatives, namely Amblyopyrum muticum (syn. Aegilops mutica) [86]. A high number of genome-wide, segmental Am.…”

Section: The Way Forwardmentioning

confidence: 99%

Harnessing Genetic Diversity of Wild Gene Pools to Enhance Wheat Crop Production and Sustainability: Challenges and Opportunities

et al. 2017

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Wild species are extremely rich resources of useful genes not available in the cultivated gene pool. For species providing staple food to mankind, such as the cultivated Triticum species, including hexaploid bread wheat (Triticum aestivum, 6x) and tetraploid durum wheat (T. durum, 4x), widening the genetic base is a priority and primary target to cope with the many challenges that the crop has to face. These include recent climate changes, as well as actual and projected demographic growth, contrasting with reduction of arable land and water reserves. All of these environmental and societal modifications pose major constraints to the required production increase in the wheat crop. A sustainable approach to address this task implies resorting to non-conventional breeding strategies, such as "chromosome engineering". This is based on cytogenetic methodologies, which ultimately allow for the incorporation into wheat chromosomes of targeted, and ideally small, chromosomal segments from the genome of wild relatives, containing the gene(s) of interest. Chromosome engineering has been successfully applied to introduce into wheat genes/QTL for resistance to biotic and abiotic stresses, quality attributes, and even yield-related traits. In recent years, a substantial upsurge in effective alien gene exploitation for wheat improvement has come from modern technologies, including use of molecular markers, molecular cytogenetic techniques, and sequencing, which have greatly expanded our knowledge and ability to finely manipulate wheat and alien genomes. Examples will be provided of various types of stable introgressions, including pyramiding of different alien genes/QTL, into the background of bread and durum wheat genotypes, representing valuable materials for both species to respond to the needed novelty in current and future breeding programs. Challenging contexts, such as that inherent to the 4x nature of durum wheat when compared to 6x bread wheat, or created by presence of alien genes affecting segregation of wheat-alien recombinant chromosomes, will also be illustrated.

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A step change in the transfer of interspecific variation into wheat from Amblyopyrum muticum

Cited by 105 publications

References 42 publications

Construction of Agropyron Gaertn. genetic linkage maps using a wheat 660K SNP array reveals a homoeologous relationship with the wheat genome

Construction of Agropyron Gaertn. genetic linkage maps using a wheat 660K SNP array reveals a homoeologous relationship with the wheat genome

Applying the latest advances in genomics and phenomics for trait discovery in polyploid wheat

Harnessing Genetic Diversity of Wild Gene Pools to Enhance Wheat Crop Production and Sustainability: Challenges and Opportunities

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