A Statistical Evaluation of Methods of Chromatogram Interpretation-GPC

Abstract

U n i v e r s i t y , Hamilton, O n t a r i o , Canada. ABSTRACT Herein i s r e p o r t e d a s t a t i s t i c a l e v a l u a t i o n of two common methods of determining t h e molecular weight c a l i b r a t i o n c u r v e u s i n g broad molecular weight d i s t r i b u t i o n s t a n d a r d s . A Monte-Carlo s i m u l a t i o n technique w a s employed t o i n v e s t i g a t e and compare e r r o r propagation i n t h e s e methods. It h a s been common p r a c t i c e t o e v a l u a t e methods of chromatogram i n t e r p r e t a t i o n on a n e n t i r e l y t h e o r e t i c a l b a s i s without c o n s i d e r a t i o n of e r r o r propagation in t h e c a l c u l a t i o n procedures employed i n t h e v a r i o u s methods. It i s p o s s i b l e f o r a method which has a sounder t h e o r e t i c a l b a s i s t o g i v e less r e l i a b l e r e s u l t s because of e x c e s s i v e e r r o r propagat i o n . A s t a t i s t i c a l e v a l u a t i o n of e r r o r p r o p a g a t i o n a l s o i d e n t i f i e s t h e s t e p s i n calcula'tion procedures where s e r i o u s e r r o r p r o p a g a t i o n o c c u r s l e a d i n g perhaps t o a l t e r n a t e and improved c a l c u l a t i o n p a t h s . The a n a l y s i s of s e v e r a l s o u r c e s of e r r o r i s p r e s e n t e d i n d e t a i l i n c l u d i n g propagation e r r o r s due t o e r r o r s i n t h e molecular weight a v e r a g e s of t h e broad molecular weight d i s t r i b u t i o n s t a n d a r d s and chromatogram r e p l i c a t i o n e r r o r .

Diels–Alder Hyperbranched Pyridylphenylene Polymer Fractions as Alternatives to Dendrimers

“…The second was done by internal calibration based on sedimentation–diffusion analysis data, and the third one was made also with sedimentation–diffusion analysis data with implementation of procedures developed for optimized internal calibration with polydisperse standards (Table S1). , …”