A standardized pre-treatment method of biofilm flocs for fluorescence microscopic characterization

Evaluation and optimization of multiple fluorophore analysis of a Pseudomonas aeruginosa biofilm

“…Multiple fluorophores have been used to characterize various bacterial communities. For example, biofilm floc characterization from drinking water treatment plants via independent subsequent scans of three fluorophores to differentiate DNA, alpha-polysaccharides and protein (Sun, et al, 2011); cell clustering in flow cell biofilms using two general protein and a DNA stain (Stewart, et al, 2007); biofilm flocs from wastewater treatment plants using fluorophores for polysaccharides, DNA and protein (Nosyk, et al, 2008); and biofilm granules formed in aerobic batch cultures using fluorophores for proteins, DNA, lipids and polysaccharides (Chen, et al, 2007). …”

Detection Techniques for Extracellular Polymeric Substances in Biofilms: A Review

“…First, the criteria for selecting fluorochromes should be established. Next, an appropriate pretreatment method for staining, which mainly includes fixation and immobilization, should be selected (Nosyk et al 2008;Adav et al 2010). The specimens are then stained; in this step, the order of staining, selection of buffer, incubation time of staining, and washing steps need to be set optimally (Chen et al 2007;Adav et al 2010).…”

Physiological Mechanisms of Imprinting and Homing Migration of Pacific Salmon

“…There are many possible factors affecting the DFAA compositions both within and beyond the stream environment, such as soils, vegetation, litter, pollen, dew, and various microbial activities (Thomas 1997). Among these factors, the roles of complex microbial communities called biofilms have been intensively investigated (Costerton et al 1994;Nosyk et al 2008). A biofilm consists of various microorganisms, and is embedded into a matrix of extracellular polymeric substances.…”