2006
DOI: 10.1016/j.aquaculture.2006.04.026
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A standardized method to propagate Cryptocaryon irritans on a susceptible host pompano Trachinotus ovatus

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Cited by 136 publications
(89 citation statements)
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“…Although the recovery rate is one of the important issues to be resolved, it appears to be acceptable in this assay: it is important to estimate the order of magnitude, not the precise number of C. irritans cells for this disease. Dan et al [17,18] have shown that the lethal dose of theronts is more than 12,000 theronts per fish for the host pompano Trachinotus ovatus. Thus, the lethal dose, which is much higher than our detection limit (20 theronts), allows us to ignore the recovery rate.…”
Section: Discussionmentioning
confidence: 98%
“…Although the recovery rate is one of the important issues to be resolved, it appears to be acceptable in this assay: it is important to estimate the order of magnitude, not the precise number of C. irritans cells for this disease. Dan et al [17,18] have shown that the lethal dose of theronts is more than 12,000 theronts per fish for the host pompano Trachinotus ovatus. Thus, the lethal dose, which is much higher than our detection limit (20 theronts), allows us to ignore the recovery rate.…”
Section: Discussionmentioning
confidence: 98%
“…We are not sure if our data reflects this variation because this was a field investigation, and many factors in the wild may affect the results. But we are confident that snubnose pompano is very susceptible to this parasite, and it has been successfully used as an experimental host to propagate C. irritans in our laboratory (Dan et al 2006).…”
Section: Discussionmentioning
confidence: 99%
“…irritans isolated from Snubnose pompano (Trachinotus blochii) in Daya Bay, Guangdong, China, had been propagated on T. ovatus in the laboratory for eight generations before being used in this study. The propagation and collection of parasite followed that of Dan et al (2006).…”
Section: Experimental Fish and Parasite Isolatementioning
confidence: 99%