A standardized method for the quantification of polysaccharides: An example of polysaccharides from Tremella fuciformis

Gao, Lei; Wang, Yifei; Zhang, Fan; Li, Shiji; Zhao, Jiaqi; Zhang, Qing; Ye, Jingrun; Ma, Yan; Wang, Zhipeng; Chen, Wei

doi:10.1016/j.lwt.2022.113860

Cited by 12 publications

(6 citation statements)

References 42 publications

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“…The absorption peaks around 1,732 and 1,615 cm −1 were the stretching vibrations of a carboxyl group (COO-), and the absorption peak around 1,078 cm −1 was owing to the stretching vibration of C-O-C and C-O-H bending vibration of pyranose ring ( Wu et al, 2022 ; Yuan et al, 2022 ). The results showed that TP was a mannose-based heterosaccharide, and its monosaccharide composition was similar to the previously reported Tremella polysaccharide, but the composition ratio was inconsistent ( Wu et al, 2019 ; Gao et al, 2022 ).…”

Section: Resultssupporting

confidence: 80%

“…The infrared spectrum of TP belongs to typical polysaccharide Wu et al, 2022;Yuan et al, 2022). The results showed that TP was a mannose-based heterosaccharide, and its monosaccharide composition was similar to the previously reported Tremella polysaccharide, but the composition ratio was inconsistent (Wu et al, 2019;Gao et al, 2022).…”

Section: Physicochemical Properties Of Tremella Fuciformis Polysaccha...supporting

confidence: 68%

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Tremella fuciformis polysaccharide reduces obesity in high-fat diet-fed mice by modulation of gut microbiota

Chen

Huang

et al. 2022

Front. Microbiol.

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Obesity is a metabolic disease associated with gut microbiota and low-grade chronic inflammation. Tremella fuciformis is a medicinal and edible fungus; polysaccharide (TP) is the main active component, which has a variety of biological activities, such as hypoglycemic and hypolipidemic. However, the anti-obesity effects and potential mechanisms of TP have never been reported. This study was conducted to elucidate the inhibitory effect of TP on high-fat diet (HFD)-induced obesity in mice. Mice were split into five groups: normal chow diet (NCD) group, NCD_TP_H group, HFD group, HFD_TP_L group and HFD_TP_H group. Our study showed that TP inhibited high-fat diet-induced weight gain and fat accumulation in mice and reduced blood glucose, hyperlipidemia and inflammation. TP also improved gut microbiota disorders by reducing the Firmicutes/Bacteroidetes ratio and modulating the relative abundance of specific gut microbiota. We also found that the anti-obesity and gut microbiota-modulating effects of TP could be transferred to HFD-fed mice via faecal microbiota transplantation (FMT), confirming that the gut microbiota was one of the targets of TP for obesity inhibition. Further studies showed that TP increased the production of short-chain fatty acids and the secretion of intestinal hormones. Our studies showed that TP inhibited obesity by modulating inflammation and the microbe-gut-brain axis, providing a rationale for developing TP to treat obesity and its complications.

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Section: Resultssupporting

confidence: 80%

Section: Physicochemical Properties Of Tremella Fuciformis Polysaccha...supporting

confidence: 68%

Tremella fuciformis polysaccharide reduces obesity in high-fat diet-fed mice by modulation of gut microbiota

Chen

Huang

et al. 2022

Front. Microbiol.

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“…The total sugars in the crude polysaccharides were determined with reference to a previously described phenol–sulfuric acid method with minor modifications [ 22 , 24 ]. A 100 μg/mL standard solution of glucose was prepared as a reference solution.…”

Section: Methodsmentioning

confidence: 99%

Biological Characteristics, Domesticated Cultivation Protocol, Antioxidant Activity, and Protective Effects against Cellular Oxidative Stress of an Underutilized Medicinal Mushroom: Fomitopsis palustris

Liang,

Dai,

Chang

et al. 2024

JoF

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Brown-rot fungus is one of the important medicinal mushrooms, which include some species within the genus Fomitopsis. This study identified wild macrofungi collected from a broad-leaved tree in Liaoning Province as Fomitopsis palustris using both morphological and molecular methods. To elucidate the potential medicinal and economic value of F. palustris, we conducted single-factor and orthogonal tests to optimize its mycelium culture conditions. Subsequently, we completed liquid culture and domestic cultivation based on these findings. Furthermore, crude polysaccharides were extracted from the cultivated fruiting bodies of F. palustris and their antioxidant activity was evaluated using chemical methods and cell-based models. The results showed that the optimal culture conditions for F. palustris mycelium were glucose as the carbon source, yeast extract powder as the nitrogen source, pH 6.0, and a temperature of 35 °C. Moreover, temperature was found to have the most significant impact on mycelial growth. The liquid strains were fermented for 6 days and then inoculated into a cultivation substrate composed of broadleaf sawdust, resulting in mature fruiting bodies in approximately 60 days. The crude polysaccharides extracted from the cultivated fruiting bodies of F. palustris (FPPs) possess in vitro scavenging abilities against DPPH radicals and OH radicals, as well as a certain ferric-reducing antioxidant power. Additionally, FPPs effectively mitigated H2O2-induced oxidative stress in RAW264.7cells by enhancing the intracellular activity of antioxidant enzymes such as SOD and CAT, scavenging excess ROS, and reducing MDA levels. This study provides preliminarily evidence of the potential medicinal and economic value of F. palustris and offers initial data for the future development and utilization of this species.

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“…Galactomannans with concentrated phenol-sulfuric acid become 5-hydroxy methyl furfural by dehydration reaction, then 5-hydroxymethyl furfural with two phenol molecules form a compound 4-[{5 (-(hydroxymethyl) furan-2-il) (4-hydroxyphenyl)methylene} cyclohexa-2,5dien-1-yliden]oxoniumyellow-orange with a wavelength of 490 nm. [21][22][23][24][25] The UV-Vis spectrophotometry method with concentrated phenol-sulphuric acid reagents has the advantage of being easy, sensitive, reproducible, and specific enough to determine carbohydrate levels compared to other methods such as gas chromatography and high-performance liquid chromatography. [10,26,27] Concentrated phenol-sulfuric acid reagents are easy-to-use, inexpensive, and sensitive reagents.…”

Section: Original Articlementioning

confidence: 99%

Untitled

2023

IJGP

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Aim: Method validation is a step to ensure that the method used is in accordance with the desired goals. The Category I validation method determines the content of the main ingredient in the drug substance. Galactomannan is a watersoluble polysaccharide composed of D-galactose and D-mannose and is the main component in guar gum. Materials and Methods: To determine galactomannan content, concentrated phenol-sulfuric acid was used using the UV-Vis Spectrophotometry method. Hydrolysis of guar gum is required to react with phenolic reagents to form colored compounds. Results and Discussion: Optimization of the formation of colored derivative compounds was carried out by hydrolysis of guar gum with the addition of 1.0 mL of sulfuric acid, 30 min time, 100°C temperature, and color stability at 180 min. Method validation was successfully carried out by testing several parameters, including selectivity with the selected wavelength 492 nm. Linear regression equation y = 0.0255 × + 0.0420 (r: 0.9998) and Vxo = 0.76%. The precision expressed in the Coefficient of Variation (C.V) is 1.06%. Accuracy was obtained 100.35 ± 0.56 (%; w/w). Conclusion:In determining the galactomannan content in guar gum powder, it was 70.00 ± 0.24 (%; w/w).

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A standardized method for the quantification of polysaccharides: An example of polysaccharides from Tremella fuciformis

Cited by 12 publications

References 42 publications

Tremella fuciformis polysaccharide reduces obesity in high-fat diet-fed mice by modulation of gut microbiota

Tremella fuciformis polysaccharide reduces obesity in high-fat diet-fed mice by modulation of gut microbiota

Biological Characteristics, Domesticated Cultivation Protocol, Antioxidant Activity, and Protective Effects against Cellular Oxidative Stress of an Underutilized Medicinal Mushroom: Fomitopsis palustris

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