A standardised protocol for the quantification of lactate dehydrogenase activity in saliva

Peña, Vı́ctor Alonso de la; Dios, Pedro Diz; Rocamonde, Santiago Lojo; Sierra, Rafael Tojo; Rodrı́guez-Segade, Santiago

doi:10.1016/s0003-9969(03)00196-1

Cited by 17 publications

(11 citation statements)

References 6 publications

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“…It is difficult to know the reason for the changes of TEA, it is likely to have been influenced by the instability of any of the various enzymes that integrate in this total activity ( 8 ). Lactate dehydrogenase results in our study were similar to other reports that showed a significant decrease of this enzyme in saliva after only 30 min, 3 days and 2 weeks of storage at - 20 ºC ( 2 , 3 , 22 ). These results could be due to the lability of the LD-4 and LD-5 isoenzymes at - 20 ºC ( 28 ).…”

Section: Discussionsupporting

confidence: 92%

Changes of salivary biomarkers under different storage conditions: effects of temperature and length of storage

Barranco

Rubio

Tvarijonaviciute

et al. 2018

Biochem. med. (Online)

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IntroductionIn this report, we aimed to examine the stability of various analytes in saliva under different storage conditions.Materials and methodsAlpha-amylase (AMY), cholinesterase (CHE), lipase (Lip), total esterase (TEA), creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LD), lactate (Lact), adenosine deaminase (ADA), Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability (FRAS), cupric reducing antioxidant capacity (CUPRAC), uric acid (UA), catalase (CAT), advanced oxidation protein products (AOPP) and hydrogen peroxide (H2O2) were colorimetrically measured in saliva obtained by passive drool from 12 healthy voluntary donors at baseline and after 3, 6, 24, 72 hours, 7 and 14 days at room temperature (RT) and 4 ºC, and after 14 days, 1, 3 and 6 months at – 20 ºC and – 80 ºC.ResultsAt RT, changes appeared at 6 hours for TEA and H2O2; 24 hours for Lip, CK, ADA and CUPRAC; and 72 hours for LD, Lact, FRAS, UA and AOPP. At 4 ºC changes were observed after 6 hours for TEA and H2O2; 24 hours for Lip and CUPRAC; 72 hours for CK; and 7 days for LD, FRAS and UA. At – 20 ºC changes appeared after 14 days for AST, Lip, CK and LD; and 3 months for TEA and H2O2. At – 80 ºC observed changes were after 3 months for TEA and H2O2.ConclusionsIn short-term storage, the analytes were more stable at 4 ºC than at room temperature, whereas in long-term storage they were more stable at - 80 ºC than at – 20 ºC.

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Section: Discussionsupporting

confidence: 92%

Changes of salivary biomarkers under different storage conditions: effects of temperature and length of storage

Barranco

Rubio

Tvarijonaviciute

et al. 2018

Biochem. med. (Online)

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“…Then, 100 μL of NADH (at a final concentrate of 0.08 mM) was added just before the change in absorbance observed after 3 minutes using a U-2910 spectrophotometer (Hitachi High-Technologies Corp., Tokyo, Japan). 14 The results were expressed in IU/L.…”

Section: Methodsmentioning

confidence: 99%

Lactate Dehydrogenase and β-Glucuronidase as Salivary Biochemical Markers of Periodontitis Among Smokers and Non-Smokers

Ali

Telgi

Tirth

et al. 2018

Sultan Qaboos Univ Med J

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Objectives: This study aimed to establish lactate dehydrogenase (LDH) and β-glucuronidase as salivary biomarkers of periodontitis among smokers and non-smokers. Methods: This cross-sectional case-control study was conducted at the Aligarh Muslim University, Aligarh, India, between January and June 2017. A total of 200 participants were divided into four groups based on their periodontal and smoking statuses. Unstimulated mixed saliva samples were collected to estimate LDH and β-glucuronidase levels. In addition, total protein was estimated using Lowry’s method. Results: There was a significant increase in enzyme activity in the periodontitis groups compared to the non-periodontitis groups (P <0.001). However, significantly lower enzyme activity was observed among smokers, irrespective of periodontal status (P <0.001). Nevertheless, a receiver operating characteristic curve analysis indicated the diagnostic potential of both enzymes to be fair-to-excellent. Conclusion: Although smoking was found to significantly alter enzyme activity, LDH and β-glucuronidase were reliable salivary biomarkers of periodontitis among both smokers and non-smokers.Keywords: Periodontitis; Biomarkers; Saliva; Lactate Dehydrogenase; beta-Glucuronidase; Smoking; Tobacco Use; India.

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“…The activity of LDH obtained in this study was considerably higher ( p < 0.05) in patients suffering from peptic ulcers ( Table 2). The centrifuged saliva samples were stored at 4 C until use since a gradual degradation of LDH may occur when stored at À20 C. 27 It has been shown that LDH activity in saliva of adults is almost similar to that of young volunteers. 28,29 We also observed that there was no change in LDH activity when various ages were compared, but due to small size of subjects it cannot be conclusive.…”

Section: Discussionmentioning

confidence: 99%

Salivary enzymes in peptic ulcer disease

Motamedi

Mansour-Ghanaei

Sariri

et al. 2013

Journal of Oral Biology and Craniofacial Research

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Peptic ulcerLactate dehydrogenase Peroxidase Amylase a b s t r a c t Aim: Peptic ulcer, the common disease of the upper gastro-intestinal tract, occurs in about 5e10% of the world's population. Therefore, diagnosis of trace disease progression with a noninvasive method is of prime importance in the field of healthcare research. The aim of this study was to evaluate the validity of salivary enzymes as noninvasive biomarkers for peptic ulcer. Materials and methods: In practice, 34 peptic ulcer patients and 30 healthy subjects donated their un-stimulated saliva samples after 8 h of fasting. The activity of some selected enzymes was measured using appropriate enzymatic assay methods. Results: The results indicated an overall alternation in enzymatic activity of saliva in patients suffering from peptic ulcer. Biological activity of a-amylase, peroxidase and lactate dehydrogenase, showed significantly higher values in almost all patients as compared to control subjects. Conclusions: Based on the results of salivary enzyme activity, it was concluded that besides the influence of their peptic ulcer on enzyme activity of saliva, the considerably higher activity of a-amylase could also be related to the major role of the enzyme on physiological oxidative stress.

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A standardised protocol for the quantification of lactate dehydrogenase activity in saliva

Cited by 17 publications

References 6 publications

Changes of salivary biomarkers under different storage conditions: effects of temperature and length of storage

Changes of salivary biomarkers under different storage conditions: effects of temperature and length of storage

Lactate Dehydrogenase and β-Glucuronidase as Salivary Biochemical Markers of Periodontitis Among Smokers and Non-Smokers

Salivary enzymes in peptic ulcer disease

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