A standard sample preparation and calibration procedure for imaging zinc and magnesium in rats' brain tissue by laser ablation-inductively coupled plasma-time of flight-mass spectrometry

Abstract

A calibration procedure consists of several steps, each of which has a significant impact on the final result of the analysis.

The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction

“…Similar to the MALDI IMS workflow, samples are thinly sectioned and mounted onto slides for analysis. A ultraviolet laser is used to systematically ablate material into a sheath gas, which carries the material to the ICP torch for ionization 24 . By visualizing elemental gradients within biofilms, portions of the biomass with differential access to nutrient metals can be identified.…”

Bioanalytics in Quantitive (Bio)imaging/Mapping of Metallic Elements in Biological Samples

“…In bioanalytical studies the biological matrix of standard analyzed samples are usually very complex and are never exactly equal to standard samples (Cuadros-Rodríguez et al, 2007;Meyers et al, 2008). Due to the lack of suitable CRMs and SRMs for (bio)imaging/mapping studies, different calibration strategies are being developed, for example, a matrix matched calibration strategy, based on the conventional extrapolative method (CEM), or extrapolative method, known popularly as the "standard addition method" (Jurowski et al, 2013(Jurowski et al, , 2014b. On the other hand, there are examples where there are commercially available CRMs or SRMs that can be used in (bio)imaging/ mapping metallic elements, such as CRM LGC 7112 for pig liver (Pornwilard et al, 2013), NIST SRM 1577b for bovine liver (Hare et al, 2010), NIST SRM 1547 for peach leaves Wu et al, 2012), MICROMATTER XRF calibration standards (universal standards for XRF analysis), and SRM NIST 1515 for apple leaves (Becker et al, 2008c).…”

“…It was revealed that the zinc concentration is reduced in the hippocampus regions in an animal depression model, and in the brains of people suffering from depression when compared to the control group (healthy people). These studies are presently being conducted by the Institute of the Polish Academy Sciences in Krak ow, with the cooperation of the Faculty of Chemistry, Jagiellonian University in Krak ow (Jurowski et al, 2013(Jurowski et al, , 2014a(Jurowski et al, , 2014b(Jurowski et al, , 2015.…”

On-line reverse isotope dilution analysis for spatial quantification of elemental labels used in immunohistochemical assisted imaging mass spectrometry via LA-ICP-MS

“…External calibration using matrix-matched laboratory standards has been applied most frequently [8][9][10][11] . With the aim to mimic the biological environment and to guarantee comparable physical and chemical behaviours in terms of ablation and ionisation characteristics, different materials have been suggested for the fabrication of matrix-matched standards including gelatin or an homogenate of a target organ such as breast or brain tissue spiked with the element of interest 8,[12][13][14] . Other quantification strategies use internal standardisation or isotope dilution analysis (IDA).…”