A stage-specific open reading frame from three-day old adult worms of<i>Trichinella spiralis</i>encodes zinc-finger motifs

Zhu, Xiaoling; García-Reyna, Patricia; Fu, Bao‐Quan; Yang, Jian; Li, C.V.; Yang, Yaping; Liu, M.Y.; Ortega‐Pierres, Guadalupe; Boireau, Pascal

doi:10.1051/parasite/2005122151

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…Poly (A)+RNAs were isolated from total RNA using a Poly (A) Quick mRNA Isolation Kit (Stratagene, USA). cDNA libraries of ML, Ad3 and Ad5 were previously constructed (Liu et al 2001; Zhu et al 2005). The cDNA library of NBL was constructed using the lamda ZAP II cDNA synthesis kit (Stratagene, USA), according to the instructions provided by the manufacturer, with minor modifications (Vayssier et al 1999).…”

Section: Methodsmentioning

confidence: 99%

“…It has been investigated whether adults at day 3 p.i. (Ad3) represent a stage without any NBL (Liu et al 2001), although they may contain some fertilized eggs in early stages of development (Zhu et al 2005). On the contrary, female adults contain all developmental stages (from fertilized eggs to NBL) of the embryo in the uterus beginning at day 4 p.i.…”

Section: Introductionmentioning

confidence: 99%

“…We thank Catherine Trap, Jean François Fabien and Qiang Lu for all of their help with the experiments, and we are grateful to Dr Isabella Valle and Dr Xingquan Zhu for their helpful comments. The cloning and analysis of the complete coding sequences of the following clones/genes have been reported elsewhere: clone T12D7, Zhu et al (2005); clone T54, Fu et al (2005 a ); clone T671, Fu et al (2005 b ).…”

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confidence: 99%

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Identification of stage-specifically expressed genes ofTrichinella spiralisby suppression subtractive hybridization

Liu

Wang

et al. 2007

Parasitology

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SUMMARYNewborn larvae (NBL) and adult (Ad) stage-specifically expressed genes or members of gene families of Trichinella spiralis were identified by suppression subtractive hybridization (SSH). Six cDNA clones were identified as NBL stage-specific, including 1 member of the T. spiralis gene family encoding glutamic acid-rich proteins, 2 clones encoding novel serine proteases, 2 closely related clones encoding proteins that are members of a deoxyribonuclease II (DNase II)-like family and 1 clone with no similarity to known genes. Four stage-specific clones encoding homologues of retinoid X receptor, caveolin, C2H2 type zinc finger protein and a putative protein with no homology to known sequences were obtained from 3-day-old adult worms. One gene specifically up-regulated in the 5-day-old adult worms encoding a putative cuticle collagen was also identified.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Identification of stage-specifically expressed genes ofTrichinella spiralisby suppression subtractive hybridization

Liu

Wang

et al. 2007

Parasitology

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“…To improve the ES-ELISA, cDNA libraries of different developmental stages of Trichinella were screened using the serum of pigs at different days post-infection (dpi), and some immunodominant antigens of T. spiralis were evaluated to detect Trichinella infection, showing a promising diagnostic potential ( Zhu et al, 2005 ; Liu et al, 2007 ; Wu et al, 2009 ; Liu P. et al, 2013 ). Interestingly, ELISA based on antigenic molecules (T668, Ts -CLP and 31 kDa antigens) could detect Trichinella infection earlier than ES antigens ( Cui et al, 2015 ; Tang et al, 2015 ).…”

Section: Diagnosismentioning

confidence: 99%

Current Research of Trichinellosis in China

Bai

Liu

et al. 2017

Front. Microbiol.

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Trichinellosis, caused by Trichinella, is an emerging or re-emerging zoonotic parasitic disease, which is distributed worldwide with major socio-economic importance in some developing countries. In particular, it has been calculated that more than 40 million people are at risk of Trichinella infection in China. This review summarizes the current information on the epidemiology, laboratory diagnosis and vaccines of trichinellosis in China. Moreover, study of the treatment potential of using Trichinella for immune-related diseases and cancer, as well as the transcription and post-transcription modification of Trichinella were also collected, providing viewpoints for future investigations. Current advances in research will help us to develop new strategies for the prevention and control of trichinellosis and may potentially yield biological agents for treating other diseases.

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“…A protein with zinc-finger domain in T. spiralis (approximately 55 kDa) is present in the crude extracts of muscle larvae, adult worms, and newborn larvae . Another protein with zinc-finger motifs is present only in the adult worms of T. spiralis, and this protein contains putative DNA-binding motifs (Hu et al 2005). …”

Section: The Proteins With Fyve Zinc-finger Domainmentioning

confidence: 99%

Functional genes and proteins of Trichinella spp.

Nagano

Takahashi

2008

Parasitol Res

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Research of Trichinella proteins has been conducted with emphasis on excretory-secretory (E-S) products of muscle larvae because of two reasons. The first is that it has prominent and narrow specific antigenicity, and the second is that it may play some role in nurse cell formation after being secreted into host muscle cells. Proteomic analysis of E-S proteins was further advanced by the aid of new analytical methods such as gene cloning, matrix-assisted laser desorption-ionization time-of-flight mass spectrometry, and expressed sequence tags database analysis. As the research progressed, the interest of researchers moved to identification of function of E-S products, which has shed further light on the intriguing relationships between parasites and hosts. Major constituents of the E-S products include 43-, 53-, and 45-kDa glycoprotein derived from the stichosome. Many proteins were discovered in E-S products after the 43-, 53-, and 45-kDa proteins although the relationships among them remain unclear. Some of the new proteins were partially defined in terms of their function including nuclear antigens, MyoD-like protein, TsJ5 protein, etc. There are better-characterized proteins based on the gene molecular method, which allow easier identification of the function of proteins of interest. Such examples were demonstrated by proteinases, proteinase inhibitors, heat shock proteins, glycosidases, etc.

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A stage-specific open reading frame from three-day old adult worms ofTrichinella spiralisencodes zinc-finger motifs

Cited by 8 publications

References 21 publications

Identification of stage-specifically expressed genes ofTrichinella spiralisby suppression subtractive hybridization

Identification of stage-specifically expressed genes ofTrichinella spiralisby suppression subtractive hybridization

Current Research of Trichinellosis in China

Functional genes and proteins of Trichinella spp.

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