A Stable Reagent Mixture for the Whole Blood Transketolase Assay

Chamberlain, B R; Buttery, J E; Pannall, P R

doi:10.1177/000456329603300413

Cited by 26 publications

(19 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thiamine and thiamine monophosphate (TMP) were determined in food, plasma, and urine by reversedphase high-performance liquid chromatography with fluorimetric detection (18). TK activity was determined in glomerular extract and hemolysate by the method of Chamberlain et al (19). For Western blotting, glomerular protein (150 g/well) was separated by SDS-PAGE (10% gels), transferred to nitrocellulose and blocked with 5% milk protein, and blotted with rabbit anti-rat TK IgG (supplied by Prof. F. Paoletti) and anti-rabbit IgG-peroxidase conjugate, and blots were developed with enhanced chemiluminescence (AP Biotech, Amersham, U.K.).…”

Section: Methodsmentioning

confidence: 99%

Prevention of Incipient Diabetic Nephropathy by High-Dose Thiamine and Benfotiamine

et al. 2003

View full text Add to dashboard Cite

Accumulation of triosephosphates arising from high cytosolic glucose concentrations in hyperglycemia is the trigger for biochemical dysfunction leading to the development of diabetic nephropathy-a common complication of diabetes associated with a high risk of cardiovascular disease and mortality. Here we report that stimulation of the reductive pentosephosphate pathway by high-dose therapy with thiamine and the thiamine monophosphate derivative benfotiamine countered the accumulation of triosephosphates in experimental diabetes and inhibited the development of incipient nephropathy. High-dose thiamine and benfotiamine therapy increased transketolase expression in renal glomeruli, increased the conversion of triosephosphates to ribose-5-phosphate, and strongly inhibited the development of microalbuminuria. This was associated with decreased activation of protein kinase C and decreased protein glycation and oxidative stress-three major pathways of biochemical dysfunction in hyperglycemia. Benfotiamine also inhibited diabetes-induced hyperfiltration. This was achieved without change in elevated plasma glucose concentration and glycated hemoglobin in the diabetic state. High-dose thiamine and benfotiamine therapy is a potential novel strategy for the prevention of clinical diabetic nephropathy.

show abstract

Section: Methodsmentioning

confidence: 99%

Prevention of Incipient Diabetic Nephropathy by High-Dose Thiamine and Benfotiamine

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Clinically, thiamine status is quantified biochemically using a TKT assay of whole blood samples [41]. This assay involves measuring the increase in the activity of the thiamine-dependent enzyme TKT after added TPP.…”

Section: Reviewmentioning

confidence: 99%

Linking vitamin B1 with cancer cell metabolism

et al. 2013

View full text Add to dashboard Cite

The resurgence of interest in cancer metabolism has linked alterations in the regulation and exploitation of metabolic pathways with an anabolic phenotype that increases biomass production for the replication of new daughter cells. To support the increase in the metabolic rate of cancer cells, a coordinated increase in the supply of nutrients, such as glucose and micronutrients functioning as enzyme cofactors is required. The majority of co-enzymes are water-soluble vitamins such as niacin, folic acid, pantothenic acid, pyridoxine, biotin, riboflavin and thiamine (Vitamin B1). Continuous dietary intake of these micronutrients is essential for maintaining normal health. How cancer cells adaptively regulate cellular homeostasis of cofactors and how they can regulate expression and function of metabolic enzymes in cancer is underappreciated. Exploitation of cofactor-dependent metabolic pathways with the advent of anti-folates highlights the potential vulnerabilities and importance of vitamins in cancer biology. Vitamin supplementation products are easily accessible and patients often perceive them as safe and beneficial without full knowledge of their effects. Thus, understanding the significance of enzyme cofactors in cancer cell metabolism will provide for important dietary strategies and new molecular targets to reduce disease progression. Recent studies have demonstrated the significance of thiamine-dependent enzymes in cancer cell metabolism. Therefore, this review discusses the current knowledge in the alterations in thiamine availability, homeostasis, and exploitation of thiamine-dependent pathways by cancer cells.

show abstract

“…The activity of the TPP-dependent enzyme transketolase was measured in hindlimb skeletal muscles (n=6 mice per group) by the kinetic method of Chamberlain et al [38].…”

Section: Spectrophotometric Assay Of Transketolase Activitymentioning

confidence: 99%

Benfotiamine accelerates the healing of ischaemic diabetic limbs in mice through protein kinase B/Akt-mediated potentiation of angiogenesis and inhibition of apoptosis

et al. 2006

View full text Add to dashboard Cite

Aims/hypothesis: Benfotiamine, a vitamin B1 analogue, reportedly prevents diabetic microangiopathy. The aim of this study was to evaluate whether benfotiamine is of benefit in reparative neovascularisation using a type I diabetes model of hindlimb ischaemia. We also investigated the involvement of protein kinase B (PKB)/Akt in the therapeutic effects of benfotiamine. Methods: Streptozotocin-induced diabetic mice, given oral benfotiamine or vehicle, were subjected to unilateral limb ischaemia. Reparative neovascularisation was analysed by histology. The expression of Nos3 and Casp3 was evaluated by real-time PCR, and the activation state of PKB/Akt was assessed by western blot analysis and immunohistochemistry. The functional importance of PKB/Akt in benfotiamine-induced effects was investigated using a dominant-negative construct.

show abstract

A Stable Reagent Mixture for the Whole Blood Transketolase Assay

Cited by 26 publications

References 4 publications

Prevention of Incipient Diabetic Nephropathy by High-Dose Thiamine and Benfotiamine

Prevention of Incipient Diabetic Nephropathy by High-Dose Thiamine and Benfotiamine

Linking vitamin B1 with cancer cell metabolism

Benfotiamine accelerates the healing of ischaemic diabetic limbs in mice through protein kinase B/Akt-mediated potentiation of angiogenesis and inhibition of apoptosis

Contact Info

Product

Resources

About