A sporulation‐specific, <scp><i>sigF</i></scp>‐dependent protein, <scp>SspA</scp>, affects septum positioning in <i><scp>S</scp>treptomyces coelicolor</i>

Tzanis, Angelos; Dalton, Kate A.; Hesketh, Andrew; Hengst, Chris D. den; Buttner, Mark J.; Thibessard, Annabelle; Kelemen, Gabriella H.

doi:10.1111/mmi.12466

Cited by 12 publications

(3 citation statements)

References 85 publications

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“…More significantly, spores were frequently double the normal length, implying an interruption in the formation of some cross-walls. Such observations have been reported previously whenever the normal process of sporulation has been perturbed ( Tzanis et al , 2014 ). In turn, the spores were less tolerant of various stresses.…”

Section: Discussionsupporting

confidence: 85%

Developmental delay in a Streptomyces venezuelae glgE null mutant is associated with the accumulation of α-maltose 1-phosphate

et al. 2016

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The GlgE pathway is thought to be responsible for the conversion of trehalose into a glycogen-like α-glucan polymer in bacteria. Trehalose is first converted to maltose, which is phosphorylated by maltose kinase Pep2 to give α-maltose 1-phosphate. This is the donor substrate of the maltosyl transferase GlgE that is known to extend α-1,4-linked maltooligosaccharides, which are thought to be branched with α-1,6 linkages. The genome of Streptomyces venezuelae contains all the genes coding for the GlgE pathway enzymes but none of those of related pathways, including glgC and glgA of the glycogen pathway. This provides an opportunity to study the GlgE pathway in isolation. The genes of the GlgE pathway were upregulated at the onset of sporulation, consistent with the known timing of α-glucan deposition. A constructed ΔglgE null mutant strain was viable but showed a delayed developmental phenotype when grown on maltose, giving less cell mass and delayed sporulation. Pre-spore cells and spores of the mutant were frequently double the length of those of the wild-type, implying impaired cross-wall formation, and spores showed reduced tolerance to stress. The mutant accumulated α-maltose 1-phosphate and maltose but no α-glucan. Therefore, the GlgE pathway is necessary and sufficient for polymer biosynthesis. Growth of the ΔglgE mutant on galactose and that of a Δpep2 mutant on maltose were analysed. In both cases, neither accumulation of α-maltose 1-phosphate/α-glucan nor a developmental delay was observed. Thus, high levels of α-maltose 1-phosphate are responsible for the developmental phenotype of the ΔglgE mutant, rather than the lack of α-glucan.

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Section: Discussionsupporting

confidence: 85%

Developmental delay in a Streptomyces venezuelae glgE null mutant is associated with the accumulation of α-maltose 1-phosphate

et al. 2016

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“…They notably encode gas vesicles, ectoine and ergothioneine biosynthesis processes (Supplementary Table S5). Moreover, several sigma factors are differentially expressed in the 'HT' condition (compared to the 'MM' condition): six are induced including SigE, a regulator of cell envelope integrity (43) and SigF which is required for sporulation (44). Ten sigma factors are repressed including the principal sigma factor SigA (Supplementary Table S5).…”

Section: Activation Of Samy Prophage and Other Mobile Genetic Element...mentioning

confidence: 99%

Characterization of a novel temperate phage facilitatingin vitrodispersal of multicellular bacteria

Jaffal,

Kortebi,

Misson

et al. 2023

Preprint

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Streptomycesare renowned for their prolific production of specialized metabolites with applications in medicine and agriculture. These multicellular bacteria present a sophisticated developmental cycle, and play a key role in soil ecology. Little is known aboutStreptomyces-phage interactions and the impact of phages onStreptomycesphysiology. In this study, we investigated the conditions governing the expression and production of ′Samy ′, a prophage found inStreptomyces ambofaciensATCC 23877. This phage is a siphovirus produced simultaneously with the activation of other mobile genetic elements. We show that Samy production increases bacterial dispersal in stress conditions, facilitating the dissemination of the lineage. Altogether, we unveiled a new property of a bacteriophage infection that it is closely linked to the multicellular community life ofStreptomycesbacteria.

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“…However, one of the 45 homologues of RsbW, RsfA (SCO4677), was found to interact with SigF, suggesting that it may be an anti-sigma factor for SigF [13]. Only two target genes under the SigF control have been identified in S. coelicolor A3(2): the whiEVIII gene for spore pigment biosynthesis, and the sspA gene for secreted lipoprotein, which plays a role in sporulation [14,15]. Interestingly, immediately downstream of the sigF gene there is a gene encoding other SigB homologues, SigN (SCO4034).…”

Section: Introductionmentioning

confidence: 99%

Cross-Recognition of Promoters by the Nine SigB Homologues Present in Streptomyces coelicolor A3(2)

Ševčı́ková

Řežuchová

Mazuráková

et al. 2021

IJMS

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In contrast to Bacillus subtilis, Streptomyces coelicolor A3(2) contains nine homologues of stress response sigma factor SigB with a major role in differentiation and osmotic stress response. The aim of this study was to further characterize these SigB homologues. We previously established a two-plasmid system to identify promoters recognized by sigma factors and used it to identify promoters recognized by the three SigB homologues, SigF, SigG, and SigH from S. coelicolor A3(2). Here, we used this system to identify 14 promoters recognized by SigB. The promoters were verified in vivo in S. coelicolor A3(2) under osmotic stress conditions in sigB and sigH operon mutants, indicating some cross-recognition of these promoters by these two SigB homologues. This two-plasmid system was used to examine the recognition of all identified SigB-, SigF-, SigG-, and SigH-dependent promoters with all nine SigB homologues. The results confirmed this cross-recognition. Almost all 24 investigated promoters were recognized by two or more SigB homologues and data suggested some distinguishing groups of promoters recognized by these sigma factors. However, analysis of the promoters did not reveal any specific sequence characteristics for these recognition groups. All promoters showed high similarity in the -35 and -10 regions. Immunoblot analysis revealed the presence of SigB under osmotic stress conditions and SigH during morphological differentiation. Together with the phenotypic analysis of sigB and sigH operon mutants in S. coelicolor A3(2), the results suggest a dominant role for SigB in the osmotic stress response and a dual role for SigH in the osmotic stress response and morphological differentiation. These data suggest a complex regulation of the osmotic stress response in relation to morphological differentiation in S. coelicolor A3(2).

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A sporulation‐specific, sigF‐dependent protein, SspA, affects septum positioning in Streptomyces coelicolor

Cited by 12 publications

References 85 publications

Developmental delay in a Streptomyces venezuelae glgE null mutant is associated with the accumulation of α-maltose 1-phosphate

Developmental delay in a Streptomyces venezuelae glgE null mutant is associated with the accumulation of α-maltose 1-phosphate

Characterization of a novel temperate phage facilitatingin vitrodispersal of multicellular bacteria

Cross-Recognition of Promoters by the Nine SigB Homologues Present in Streptomyces coelicolor A3(2)

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