A splicing site mutation in BrpFLC1 and repressed expression of BrpFLC genes are associated with the early flowering of purple flowering stalk

Hu, G. L.; Hu, Zongli; Li, Y.; Gu, Fei; Zhao, Zhiping; Chen, G. P.

doi:10.1134/s1021443711030058

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…However, alleles with splice-site mutations have, to our knowledge, not been described in A. thaliana . It is striking that FLC alleles with splice-site mutations have now been found in three other species, in B. rapa , C. bursa-pastoris , and C. rubella (this work; Slotte et al 2009 ; Yuan et al 2009 ; Hu et al 2011 ).…”

Section: Discussionmentioning

confidence: 52%

“…There is evidence for FRI or FLC homologs being responsible for quantitative trait loci (QTL) affecting flowering in A. lyrata , Brassica napus , and B. rapa , with the situation being less clear for B. oleracea ( Schranz et al 2002 ; Pires et al 2004 ; Long et al 2007 ; Okazaki et al 2007 ; Razi et al 2007 ; Kuittinen et al 2008 ; Zhao et al 2010b ; Wang et al 2011 ). While the potential molecular causes for a change in FLC function in these cases remain uncertain, splice-site mutations in FLC homologs have been linked to earlier flowering in B. rapa and Capsella bursa-pastoris ( Slotte et al 2009 ; Yuan et al 2009 ; Hu et al 2011 ). However, in all these cases there has been little direct proof for a causal role of FLC or FRI alleles in flowering-time variation.…”

Section: Discussionmentioning

confidence: 99%

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IndependentFLCMutations as Causes of Flowering-Time Variation inArabidopsis thalianaandCapsella rubella

et al. 2012

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Capsella rubella is an inbreeding annual forb closely related to Arabidopsis thaliana, a model species widely used for studying natural variation in adaptive traits such as flowering time. Although mutations in dozens of genes can affect flowering of A. thaliana in the laboratory, only a handful of such genes vary in natural populations. Chief among these are FRIGIDA (FRI) and FLOWERING LOCUS C (FLC). Common and rare FRI mutations along with rare FLC mutations explain a large fraction of flowering-time variation in A. thaliana. Here we document flowering time under different conditions in 20 C. rubella accessions from across the species’ range. Similar to A. thaliana, vernalization, long photoperiods and elevated ambient temperature generally promote flowering. In this collection of C. rubella accessions, we did not find any obvious loss-of-function FRI alleles. Using mapping-by-sequencing with two strains that have contrasting flowering behaviors, we identified a splice-site mutation in FLC as the likely cause of early flowering in accession 1408. However, other similarly early C. rubella accessions did not share this mutation. We conclude that the genetic basis of flowering-time variation in C. rubella is complex, despite this very young species having undergone an extreme genetic bottleneck when it split from C. grandiflora a few tens of thousands of years ago.

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 99%

IndependentFLCMutations as Causes of Flowering-Time Variation inArabidopsis thalianaandCapsella rubella

et al. 2012

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“…FLC, however, plays a more important role in natural variation for flowering time in other species of the Brassicaceae family (Slotte et al, 2009;Wang et al, 2009;Yuan et al, 2009;Hu et al, 2011;Leinonen et al, 2013;Raman et al, 2016). Sequence analysis of the FRI locus in C. rubella did not reveal the same level of allelic variation that is observed in A. thaliana (Guo et al, 2012).…”

Section: Allelic Variation At Flc Contributes To Natural Variation Inmentioning

confidence: 82%

“…Allelic variation at FLC and associated variation in gene expression has been shown to be associated with flowering time in A. thaliana relatives as well, including C. rubella (Slotte et al, 2009;Yuan et al, 2009;Hu et al, 2011;Guo et al, 2012;Raman et al, 2016). In a previous study, we reported an allele of FLC that causes early flowering in C. rubella (Guo et al, 2012), in which a single nucleotide polymorphism (SNP) deleted a splice acceptor site, that results in a frameshift and ultimately leading to a truncated, nonfunctional FLC product.…”

Section: Introductionmentioning

confidence: 99%

Parallel Evolution of Common Allelic Variants Confers Flowering Diversity in Capsella rubella

et al. 2018

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Flowering time is an adaptive life history trait. , a close relative of and a young species, displays extensive variation for flowering time but low standing genetic variation due to an extreme bottleneck event, providing an excellent opportunity to understand how phenotypic diversity can occur with a limited initial gene pool. Here, we demonstrate that common allelic variation and parallel evolution at the locus confer variation in flowering time in We show that two overlapping deletions in the 5' untranslated region (UTR) of , which are associated with local changes in chromatin conformation and histone modifications, reduce its expression levels and promote flowering. We further show that these two pervasive variants originated independently in natural populations after speciation and spread to an intermediate frequency, suggesting a role of this parallel -regulatory change in adaptive evolution. Our results provide an example of how parallel mutations in the same 5' UTR region can shape phenotypic evolution in plants.

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“…chinensis var. communis) (Hu et al, 2011). Additionally, in an early flowering trifoliate orange (Poncirus trifoliata) mutant, five alternatively spliced transcripts of PtFLC have been identified; furthermore, their abundances are variable at the juvenile and adult stages, suggesting that the AS of PtFLC is related to flowering time (Zhang et al, 2009).…”

Section: As Of Flc Sense Transcripts Mediates Flowering Timementioning

confidence: 99%

RNA Splicing of FLC Modulates the Transition to Flowering

Lin

Ren

et al. 2019

Front. Plant Sci.

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Flowering is a critical stage of plant development and is closely correlated with seed production and crop yield. Flowering transition is regulated by complex genetic networks in response to endogenous and environmental signals. FLOWERING LOCUS C (FLC) is a central repressor in the flowering transition of Arabidopsis thaliana. The regulation of FLC expression is well studied at transcriptional and post-transcriptional levels. A subset of antisense transcripts from FLC locus, collectively termed cold-induced long antisense intragenic RNAs (COOLAIR), repress FLC expression under cold exposure. Recent studies have provided important insights into the alternative splicing of COOLAIR and FLC sense transcripts in response to developmental and environmental cues. Herein, at the 20th anniversary of FLC functional identification, we summarise new research advances in the alternative splicing of FLC sense and antisense transcripts that regulates flowering.

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A splicing site mutation in BrpFLC1 and repressed expression of BrpFLC genes are associated with the early flowering of purple flowering stalk

Cited by 8 publications

References 21 publications

IndependentFLCMutations as Causes of Flowering-Time Variation inArabidopsis thalianaandCapsella rubella

IndependentFLCMutations as Causes of Flowering-Time Variation inArabidopsis thalianaandCapsella rubella

Parallel Evolution of Common Allelic Variants Confers Flowering Diversity in Capsella rubella

RNA Splicing of FLC Modulates the Transition to Flowering

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