A spiral microfluidic chip endows high efficiency single cell alignment at extremely low flow for ICP-MS analysis

Single Cell Phenotypic Analysis for Cancer Stem Cell Identification by Dual-Isotope ICP-QMS

“…The prepared cells (about 1.5 × 10 6 cells) were incubated with aptamer-functionalized nanoparticle probes (1.5 nM EpCAM-AuNP conjugates or 1 nM MUC1-AgNP conjugates) in 200 μL of buffer on ice for 30 min. For efficient single cell dispersion and sorting, a spiral channel microfluidic chip previously fabricated in the authors’ laboratory − (the parameters and fabrication are described in Supporting Information Text S6) was coupled to the dual-isotope ICP-QMS system (Figure S1), which further guaranteed high-throughput single cell analysis at a low flow rate of 10 μL/min. The single cell ICP-QMS (SC-ICP-QMS) measurements were performed by using an Agilent 8900 ICP-MS spectrometer (Agilent 8900, Agilent Technologies, CA) equipped with a quadrupole mass analyzer in time-resolved analysis (TRA) mode.…”

Interaction of Cellular Uptake of Nanosilver and Metallothionein Stress Expression Elucidated by 2D Single-Cell Analyses Based on LIF and ICP-MS

“…The single-cell high-throughput 2D analysis platform is the hyphenation of a domain-limited spiral microfluidic single-cell focusing chip with a highly sensitive LIF unit, a data acquisition and processing system, and an ICP-MS detector. Our previous studies have demonstrated that the microfluidic chip may achieve stable focusing of a single particle or cell. , Herein, a spiral microfluidic chip with a periodic domain-limiting structure was developed and applied to achieve single-cell focusing at low flow velocities, which is particularly suitable for 2D single-cell analysis.…”

“…Set-Up of the 2D Single-Cell Analysis System. The single-cell high-throughput 2D analysis platform is the hyphenation of a domain-limited spiral microfluidic singlecell focusing chip 34 with a highly sensitive LIF unit, a data acquisition and processing system, and an ICP-MS detector. Our previous studies have demonstrated that the microfluidic chip may achieve stable focusing of a single particle or cell.…”

“…26−30 Inductively coupled plasma mass spectrometry (ICP-MS) provides ultrahigh sensitivity for the detection of metal species, and time-resolved TRA-ICP-MS plays a key role in single-cell detection when matching with appropriate dwell time and sampling flow rate. 31−33 In the present study, we report a two-dimensional (2D) high-throughput single-cell analysis platform (LIF hyphenating with ICP-MS), 25 in combination with a domain-limited spiral microfluidic chip 34 for quantifying MT stress expression in LX-2, HepG-2, SK-HEP-1, Huh-7, and MDA-MB-231 cells exposed to Ag + /AgNPs (20 nm)/PVP-AgNPs (20 nm). ICP-MS spikes reflect the uptake of Ag + /AgNPs/PVP-AgNPs in a specific cell, while LIF exploits the stress expression level of MTs in the same cell.…”

“…In the present study, we report a two-dimensional (2D) high-throughput single-cell analysis platform (LIF hyphenating with ICP-MS), in combination with a domain-limited spiral microfluidic chip for quantifying MT stress expression in LX-2, HepG-2, SK-HEP-1, Huh-7, and MDA-MB-231 cells exposed to Ag + /AgNPs (20 nm)/PVP-AgNPs (20 nm). ICP-MS spikes reflect the uptake of Ag + /AgNPs/PVP-AgNPs in a specific cell, while LIF exploits the stress expression level of MTs in the same cell.…”