A spectrophotometric micro-method for the enzymatic determination of beta-hydroxybutyrate

Chandrasekaran, Appavu; Glover, R.; Lord, P.S.

doi:10.1016/0009-8981(72)90457-3

Cited by 17 publications

(5 citation statements)

References 3 publications

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“…Blood samples were taken from the jugular vein of lambs at the start of the trial and prior to leaving the farm for slaughter. Blood serum D-|3-hydroxybutyrate (p"OH) was estimated by a reaction-rate method based on methods described by Chandrasekaran, Glover and Lord (1972) and Chandrasekaran and Lord (1973).…”

Section: Methodsmentioning

confidence: 99%

Effects of fish-meal supplementation on performance of overfat lambs fed on barley straw to reduce carcass fatness

Vipond¹,

King²,

Ørskov³

et al. 1989

Anim. Prod.

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1989). Effects of sh-meal supplementation on performance of overfat lambs fed on barley straw to reduce carcass fatness. Animal Production, 48, pp 131-138 ABSTRACT The effect of undegradable protein supply was studied in overfat lambs given low-energy diets to reduce carcass fatness. Two trials involving sequential batches of 48 overfat Suffolk x lambs investigated the effects of supplementing an ad libitum straw diet with either 0 or 100 g fish-meal pellets daily for 14-, 28-and 42-day periods. Lambs supplemented with fish meal in trials 1 and 2 lost significantly less weight. Weight losses (g/day) were -2 v. -129 (s.e.d. 26) and -58 v. -188 (s.e.d. 29) respectively. There were highly significant and favourable effects of fish-meal supplementation on carcass weight and composition (recorded in the second trial only). Carcass weight (kg) after 14, 28 and 42 days was 21-8, 20-4 and 18-3 for unsupplemented lambs and 22-4, 21-9 and 21-6 for supplemented lambs; saleable lean (kg) as determined by a commercial boning-out process was 12-1, 11-3 and 10-0 v. 12-7, 12-6 and 12-3 respectively. There were corresponding changes in conformation but fat trimmed off the carcass was not significantly reduced by the dietary treatments. There was, however, a reduction by one-fifth in excess carcass fat (about 0-5 kg) over the period 14 to 42 days on trial, and over the trial as a whole, fat trim fell from an estimated proportion of carcass weight of 0-17 to 012, equivalent to a fall in Meat and Livestock Commission fatness score from 4H to 3L. Results indicated very favourable financial rewards for supplementing diets of overfat lambs with fish meal.

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Section: Methodsmentioning

confidence: 99%

Effects of fish-meal supplementation on performance of overfat lambs fed on barley straw to reduce carcass fatness

Vipond¹,

King²,

Ørskov³

et al. 1989

Anim. Prod.

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“…On alternate specimens the concentrations were determined of blood lactate (Gutmann & Wahlefeld, 1974), plasma fl-hydroxybutyrate (Chandrasekaran, Glover & Lord, 1972), serum magnesium (by atomic absorption spectrophotometry) and serum insulin (C.I.S. Sorin Kit, Saluggia, Italy).…”

Section: Methodsmentioning

confidence: 99%

Metabolic and cardiovascular side effects of the beta 2‐adrenoceptor agonists salbutamol and rimiterol.

Phillips¹,

Vedig²,

Jones³

et al. 1980

Brit J Clinical Pharma

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The metabolic and cardiovascular side‐effects of intravenous infusions of therapeutic doses of beta 2‐adrenoceptor agonists salbutamol and rimiterol have been determined in four healthy male subjects. There were dose‐related increases in plasma glucose, renin activity, serum insulin and heart rate, and significant hyperlactataemia and ketonaemia. There were dose‐related decreases in plasma potassium, phosphate and corticosteroids and significant hypocalcaemia and hypomagnesaemia. The effects of equivalent molar amounts of salbutamol and rimiterol were similar. Whichever drug is used, special care is required with patients who may have abnormal glucose tolerance, potassium depletion, or be predisposed to lactic acidosis. Rimiterol may be preferable for infusion because of its short plasma half‐life.

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“…Endocrine assessment included measurement of the following serum parameters by standard radioassay procedures in the fasting state: thyroxine, resin-T3 uptake, thyroid-stimulating hormone, growth hormone, and cortisol (A.M. and P.M.), and these were all normal. A 100 g, 5-h oral glucose tolerance test was carried out after a 14-h fast, and in addition to glucose (9), the following measurements were made on blood taken at 0, 0.5, 1.0, 1.5, 2.0, 3.0, 4.0, and 5.0 h: glycerol, non-esterified fatty acids (NEFA) (10), ,8-hydroxybutyrate (11), lactate (12), glucagon, using Unger antibody 30K (13), and insulin (14). Serum electrolytes and bicarbonate were determined using the Stat-Ion procedure ( (16) and with sulfosalicylic acidferric chloride to detect phosphates (17).…”

Section: Case Reportmentioning

confidence: 99%

Familial hyperglycerolemia.

Rose¹,

Haines²

1978

J. Clin. Invest.

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A spectrophotometric micro-method for the enzymatic determination of beta-hydroxybutyrate

Cited by 17 publications

References 3 publications

Effects of fish-meal supplementation on performance of overfat lambs fed on barley straw to reduce carcass fatness

Effects of fish-meal supplementation on performance of overfat lambs fed on barley straw to reduce carcass fatness

Metabolic and cardiovascular side effects of the beta 2‐adrenoceptor agonists salbutamol and rimiterol.

Familial hyperglycerolemia.

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