A spectrophotometric method for simultaneous determination of protein-bound hexoses and fucose with a mixture of l-cysteine and phenol

Djurdjic, V.; Mandić, Lj.

doi:10.1016/0003-2697(90)90556-o

Cited by 22 publications

(6 citation statements)

References 10 publications

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“…Membrane-bound hexoses and fucose were determined by a slight modification of the procedure described by Djurdjic and Mandic ( 18 ). Sialic acid content was determined by the method of Skoza and Mohos ( 19 ).…”

Section: Methodsmentioning

confidence: 99%

Folate Deficiency Results in Alteration in Intestinal Brush Border Membrane Composition and Enzyme Activities in Weanling Rats

Singla

Kaushik²,

Kaur³

2006

J Nutr Sci Vitaminol

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Summary Folic acid deficiency is the most prevalent vitamin deficiency throughout the world and its effect on brush border membrane composition has not been studied earlier. We investigated the effect of folate deficiency on the structure and function of the intestinal brush border membrane. Various brush border enzyme activities, membrane sugars and lipids were evaluated in two groups of weanling male albino rats after 3 mo of feeding control and folate deficient diets. Except sucrase, all the other three enzymes, viz., alkaline phosphatase, leucine amino peptidase and ␥ -glutamyl transpeptidase showed decrease in activity in rats fed folate-deficient diets. Among sugars, hexoses and hexosamines showed significant decline in amount whereas sialic acid content showed great increase in brush border membrane of folate-deficient rats as compared to controls. Furthermore, there was a significant reduction in cholesterol, phospholipids, triglycerides, cerebrosides and fucolipids in the group fed the folate-deficient diet. Our study suggests that folate deficiency results in altered enzyme activities, lipid and sugar composition of intestinal brush border membrane. Such changes might reflect the underlying cause of the gastrointestinal disturbances observed in folate deficiency.

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Section: Methodsmentioning

confidence: 99%

Folate Deficiency Results in Alteration in Intestinal Brush Border Membrane Composition and Enzyme Activities in Weanling Rats

Singla

Kaushik²,

Kaur³

2006

J Nutr Sci Vitaminol

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“…Membrane‐bound hexoses and fucose were determined as described with slight modification 23 . Briefly, 200 µL of membrane sample corresponding to approximately 10–20 µg of membrane protein was taken, and to this 1 mL of cooled mixture consisting of six parts concentrated sulfuric acid and one part water was added.…”

Section: Methodsmentioning

confidence: 99%

Simple method of preparation of rat colonocyte apical membranes using polyethylene glycol precipitation

Ramamoorthy

Balasubramanian

2003

J of Gastro and Hepatol

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“…Thereafter, an aqueous l-cysteine solution is added, and the absorbance is measured at two wavelengths, namely, 396 and 430 nm. According to the difference of those two measurements, the possible interference of hexoses can be excluded [75]. However, algal polyphenols may interfere to a great extent in colorimetric fucose determination.…”

Section: Fucose Contentmentioning

confidence: 99%

Fucoidan Characterization: Determination of Purity and Physicochemical and Chemical Properties

et al. 2020

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Fucoidans are marine sulfated biopolysaccharides that have heterogenous and complicated chemical structures. Various sugar monomers, glycosidic linkages, molecular masses, branching sites, and sulfate ester pattern and content are involved within their backbones. Additionally, sources, downstream processes, and geographical and seasonal factors show potential effects on fucoidan structural characteristics. These characteristics are documented to be highly related to fucoidan potential activities. Therefore, numerous chemical qualitative and quantitative determinations and structural elucidation methods are conducted to characterize fucoidans regarding their physicochemical and chemical features. Characterization of fucoidan polymers is considered a bottleneck for further biological and industrial applications. Consequently, the obtained results may be related to different activities, which could be improved afterward by further functional modifications. The current article highlights the different spectrometric and nonspectrometric methods applied for the characterization of native fucoidans, including degree of purity, sugar monomeric composition, sulfation pattern and content, molecular mass, and glycosidic linkages.

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A spectrophotometric method for simultaneous determination of protein-bound hexoses and fucose with a mixture of l-cysteine and phenol

Cited by 22 publications

References 10 publications

Folate Deficiency Results in Alteration in Intestinal Brush Border Membrane Composition and Enzyme Activities in Weanling Rats

Folate Deficiency Results in Alteration in Intestinal Brush Border Membrane Composition and Enzyme Activities in Weanling Rats

Simple method of preparation of rat colonocyte apical membranes using polyethylene glycol precipitation

Fucoidan Characterization: Determination of Purity and Physicochemical and Chemical Properties

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