A Spectrophotometric comparative evaluation of the sealing ability of various perforation repair materials with a novel eggshell modified GIC

Abstract

Objectives: The objective of this study was to evaluate and compare the sealing ability of mineral trioxide aggregate (MTA), Biodentine, glass ionomer cement, and glass ionomer cement modified with Chicken Eggshell Powder when used as furcation perforation repair material. Materials and Methods: In the present study, 80 human lower first molars were used. Collected teeth had no caries or restoration, and none had fused roots. Every molar had an endodontic access cavity made utilizing a high-speed, long-shank round bur for the initial entry and an Endo-Z for lateral extension and finishing the cavity walls. Each canal’s orifice was covered with a temporary filling material. The pulpal floor and cavity walls of every molar were thoroughly coated with two successive coats of clear nail polish. A significant perforation was made between the orifices to the furcation area using a high-speed long shank round bur #4. The perforation centered between the mesial and distal orifice. They were divided into four experimental groups: Group I: 20 molars were repaired using MTA, Group II: 20 molars with Biodentine, Group III: 20 molars were repaired with glass ionomer cement, and Group IV was repaired using glass ionomer cement modified with 7% chicken eggshell powder. Moist cotton pellets were placed over the repair materials, and molars were kept in 100% humidity for 24 h to allow materials to set. Then, according to each group, molars were put in Petri dishes. Methylene blue dye was applied inside the access cavity of all samples for 24 h. Molars were placed under running tap water for 30 min to remove all residues of methylene blue, and then varnish was removed with a Parker blade #15 and polishing discs. Molars were placed in vials containing 1 mL of concentrated (65 wt%) nitric acid for 3 days. Vials were centrifuged at 14,000 rpm for 5 min. The supernatant from each sample was transferred in a quantity of 200 L to a 96-well plate. Sample absorbance was read by an automatic microplate spectrophotometer at 550 nm using concentrated nitric acid as a blank. Statistical analysis was performed using one-way analysis of variance. When the analysis of variance test was significant, the pairwise comparison of the means was done using a Duncan post hoc test. The significance level was set at P < 0.05. Statistical analysis was performed with SPSS 14.0 (SPSS Inc., Chicago, IL, USA) for Windows (Microsoft, Redmond, WA, USA). Results: The highest dye absorbance was seen in Group III, followed by Groups IV, II, and I. Conclusion: Within the limitations of study it was concluded that maximum sealing ability was seen in Biodentine followed by MTA, Glass Ionomer Cement modified with 7% Chicken Eggshell powder and Glass Ionomer Cement.