A spectral model for signal elements isolated from zebrafish photopic electroretinogram

Nelson, Ralph; Singla, Nirmish

doi:10.1017/s0952523809990113

Cited by 33 publications

(74 citation statements)

References 45 publications

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“…Behavioral responses to UV light are found in turtle (Arnold and Neumeyer 1987), roach (fish) (Douglas 1986), and birds (Hart 2001;Smith et al 2002;reviewed in Goldsmith 1994) and zebrafish (Risner et al 2006). ERG recordings identify UV cone contributions in zebrafish (Bilotta et al 2005;Hughes et al 1998;Nelson and Singla 2009) and juvenile goldfish (Chen and Stark 1994). Horizontal cell hyperpolarizations to UV light have been reported in turtle (Ammermuller et al 1998;Ventura et al 1999;Zana et al 2001) and fish (Hashimoto et al 1988).…”

Section: Uv Light Responsesmentioning

confidence: 99%

“…Each cone signal is represented as an independently saturable Hill function (Nelson and Singla 2009). The model does not differentiate synaptic pathways of individual cone inputs but says simply that, whatever the pathway, the signals arising from different cone colors are separately saturable.…”

Section: Spectral Modelmentioning

confidence: 99%

“…The Palacios nomogram (Palacios et al 1998) was used for the UV cone. The coefficients of the order 8 polynomials used to calculate the absorption nomograms can be found in Nelson and Singla (2009). A nonlinear least squares method (NLSM, Origin 8.0, OriginLab, North Hampton, MA) fit horizontal cell data sets to the model (Eq.…”

Section: Spectral Modelmentioning

confidence: 99%

“…MEM is an Earles salts, bicarbonate-buffered, cell culture medium containing amino acid and vitamin supplementation. Stable ERG b-wave responses are recorded from zebrafish eyecups for periods in excess of 10 h using this medium (Nelson and Singla 2009).…”

Section: Preparation and Perfusion Of Zebrafish Retina Eyecupsmentioning

confidence: 99%

“…In trichromats, C-type triphasic responses depolarize for midspectral stimuli selective for green cones. The depolarization is flanked by long and short wavelength hyperpolarizations arising from red and blue cones (Djamgoz 1984;Djamgoz and Ruddock 1979;Fukurotani and Hashimoto 1984;Gottesman and Burkhardt 1987;Hashimoto and Inokuchi 1981;Hashimoto et al 1988;Norton et al 1968;Ohtsuka and Kouyama 1986;Yazulla 1976).…”

Section: Introductionmentioning

confidence: 99%

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Spectral Responses in Zebrafish Horizontal Cells Include a Tetraphasic Response and a Novel UV-Dominated Triphasic Response

Connaughton

Nelson

2010

Journal of Neurophysiology

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Connaughton VP, Nelson R. Spectral responses in zebrafish horizontal cells include a tetraphasic response and a novel UV-dominated triphasic response. J Neurophysiol 104: 2407-2422, 2010. First published July 7, 2010 doi:10.1152/jn.00644.2009. Zebrafish are tetrachromats with red (R, 570 nm), green (G, 480 nm), blue (B, 415 nm), and UV (U, 362 nm) cones. Although neurons in other cyprinid retinas are rich in color processing neural circuitry, spectral responses of individual neurons in zebrafish retina, a genetic model for vertebrate color vision, are yet to be studied. Using dye-filled sharp microelectrodes, horizontal cell voltage responses to light stimuli of different wavelengths and irradiances were recorded in a superfused eyecup. Spectral properties were assessed both qualitatively and quantitatively. Six spectral classes of horizontal cell were distinguished. Two monophasic response types (L1 and L2) hyperpolarized at all wavelengths. L1 sensitivities peaked at 493 nm, near the G cone absorbance maximum. Modeled spectra suggest equally weighted inputs from both R and G cones and, in addition, a "hidden opponency" from blue cones. These were classified as RϪ/GϪ/(bϩ). L2 sensitivities were maximal at 563 nm near the R cone absorbance peak; modeled spectra were dominated by R cones, with lesser G cone contributions. B and UV cone signals were small or absent. These are RϪ/gϪ. Four chromatic (C-type) horizontal cells were either depolarized (ϩ) or hyperpolarized (Ϫ) depending on stimulus wavelength. These types are biphasic (Rϩ/GϪ/BϪ) with peak excitation at 467 nm, between G and B cone absorbance peaks, UV triphasic (rϪ/Gϩ/ UϪ) with peak excitation at 362 nm similar to UV cones, and blue triphasic (rϪ/Gϩ/BϪ/uϪ) and blue tetraphasic (rϪ/Gϩ/BϪ/uϩ), with peak excitation at 409 and 411 nm, respectively, similar to B cones. UV triphasic and blue tetraphasic horizontal cell spectral responses are unique and were not anticipated in previous models of distal color circuitry in cyprinids.

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Section: Uv Light Responsesmentioning

confidence: 99%

Section: Spectral Modelmentioning

confidence: 99%

Section: Spectral Modelmentioning

confidence: 99%

Section: Preparation and Perfusion Of Zebrafish Retina Eyecupsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Spectral Responses in Zebrafish Horizontal Cells Include a Tetraphasic Response and a Novel UV-Dominated Triphasic Response

Connaughton

Nelson

2010

Journal of Neurophysiology

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Cone signals in monostratified and bistratified amacrine cells of adult zebrafish retina

Torvund

Connaughton

et al. 2016

J of Comparative Neurology

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Strata within the inner plexiform layer (IPL) of vertebrate retinas are suspected to be distinct signaling regions. Functions performed within adult zebrafish IPL strata were examined through microelectrode recording and staining of stratified amacrine types. The stimulus protocol and analysis discriminated the pattern of input from red, green, blue, and UV cones as well as the light-response waveforms in this tetrachromatic species. A total of 36 cells were analyzed. Transient depolarizing waveforms at ON and OFF originated with bistratified amacrine types, whose dendritic planes branched either in IPL sublaminas a & b, or only within sublamina a. Monophasic-sustained depolarizing waveforms originated with types monostratified in IPL s4 (sublamina b). OFF responses hyperpolarized at onset, depolarized at offset, and in some cases depolarized during mid-stimulus. These signals originated with types monostratified in s1 or s2 (sublamina a). Bistratified amacrines received depolarizing signals only from red cones, at both ON and OFF, while s4 stratified ON cells combined red and green cone signals. The s1/s2 stratified OFF cells utilized hyperpolarizing signals from red, red and green, or red and blue cones at ON, but only depolarizing red cone signals at OFF. ON and OFF depolarizing transients from red cones appear widely distributed within IPL strata. “C-type” physiologies, depolarized by some wavelengths, hyperpolarized by others, in biphasic or triphasic spectral patterns, originated with amacrine cells monostratified in s5. Collectively, cells in this stratum processed signals from all cone types.

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Assessing Rewiring of the Retinal Circuitry by Electroretinogram (ERG) After Inner Retinal Lesion in Adult Zebrafish

Barrett

Meighan

Mitchell

et al. 2023

Methods in Molecular Biology

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A spectral model for signal elements isolated from zebrafish photopic electroretinogram

Cited by 33 publications

References 45 publications

Spectral Responses in Zebrafish Horizontal Cells Include a Tetraphasic Response and a Novel UV-Dominated Triphasic Response

Spectral Responses in Zebrafish Horizontal Cells Include a Tetraphasic Response and a Novel UV-Dominated Triphasic Response

Cone signals in monostratified and bistratified amacrine cells of adult zebrafish retina

Assessing Rewiring of the Retinal Circuitry by Electroretinogram (ERG) After Inner Retinal Lesion in Adult Zebrafish

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