A spectral hole‐burning study of long‐wavelength chlorophyll <i>a</i> forms in greening leaves at 5 K

Mauring, Koit; Renge, Indrek; Avarmaa, R.

doi:10.1016/0014-5793(87)80529-x

Cited by 12 publications

(4 citation statements)

References 20 publications

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“…For stationary emission polarized parallel and perpendicular to e, we get (30) Iil( vexc, v.c) = I Fi(Vrec)DijGijAj ( Vexc ) i,j Ii(Vexcq Vrec) = 2.5I(Vexc, "rec) O5Ii(Vexc, vrec) (31) III. SIMULATIONS To prove the working power of our model, numerous model simulations have been performed with different initial conditions and parameters of the model.…”

Section: Polarizationmentioning

confidence: 99%

“…For bacterial antenna, very few data are available (26). Assuming that electron-phonon coupling parameters are nearly the same in case of antenna bacteriochlorophyll-proteins and chlorophyll-proteins, the results obtained in PS-I particles (27) and in greening leaves (30) are taken into account in our calculations. The zero phonon line F is taken equal to 5 cm-' (the exact value of this parameter has far less influence on excitation ;-r -CD C 6 FIGURE 3 Dependence of the transfer rate on the energy difference vij and temperature, relative to the rate at 300 K if vij = 0.…”

Section: Parametersmentioning

confidence: 99%

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Kinetic model of primary energy transfer and trapping in photosynthetic membranes

Pullerits

Freiberg

1992

Biophysical Journal

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The picosecond time-domain incoherent singlet excitation transfer and trapping kinetics in core antenna of photosynthetic bacteria are studied in case of low excitation intensities by numerical integration of the appropriate master equation in a wide temperature range of 4-300 K. The essential features of our two-dimensional-lattice model are as follows: Förster excitation transfer theory, spectral heterogeneity of both the light-harvesting antenna and the reaction center, treatment of temperature effects through temperature dependence of spectral bands, inclusion of inner structure of the trap, and transition dipole moment orientation. The fluorescence kinetics is analyzed in terms of distributions of various kinetic components, and the influence of different inhomogeneities (orientational, spectral) is studied.A reasonably good agreement between theoretical and experimental fluorescence decay kinetics for purple photosynthetic bacterium Rhodospirillum rubrum is achieved at high temperatures by assuming relatively large antenna spectral inhomogeneity: 20 nm at the whole bandwidth of 40 nm. The mean residence time in the antenna lattice site (it is assumed to be the aggregate of four bacteriochlorophyll a molecules bound to proteins) is estimated to be approximately 12 ps. At 4 K only qualitative agreement between model and experiment is gained. The failure of quantitative fitting is perhaps due to the lack of knowledge about the real structure of antenna or local heating and cooling effects not taken into account.

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Section: Polarizationmentioning

confidence: 99%

Section: Parametersmentioning

confidence: 99%

Kinetic model of primary energy transfer and trapping in photosynthetic membranes

Pullerits

Freiberg

1992

Biophysical Journal

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“…More recently, FLNS has been applied to numerous biomolecules and biological systems: chlorophylls o and b (13)(14)(15) pheophytin a (16,17), protochlorophyll and protopheophytin (18), heme proteins (19), iron-free cytochrome c (20), etiolated leaves (21), and bacteriochlorophyll a and bacteriopheophytin a (22). The closely related line narrowing technique of spectral hole burning (10, [23][24][25] has now been applied to a wider variety of photosynthetic protein-pigment complexes (14,22,(26)(27)(28)(29)(30). For such complexes, also lies in the aforementioned range and it is clear that there is a significant contribution to rinh from statistical fluctuations in protein-pigment structure.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence line narrowing: a high-resolution window on DNA and protein damage from chemical carcinogens

Jankowiak¹,

Small²

1991

Chem. Res. Toxicol.

114

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“…The FLN spectra shown in Figure have been measured by scanning the narrow-band laser excitation in the region of vibronic absorption and by detecting the emission through a narrow-slit monochromator at the maxima of the respective tautomeric origin bands (B 1 , B 2 , B 3 ). General discussion of the FLN technique has been given in numerous papers and reviews, ,, and, similarly, the VHB technique has been discussed and applied by several authors. − For interpretation of our experiments, however, a short overview of basic principles may be useful.…”

Section: Discussionmentioning

confidence: 99%

Variety in the Coupling of Mesoporphyrin IX to Apohorseradish Peroxidase C Studied by Energy Selected Fluorescence Excitation and Vibronic Hole Burning Spectroscopy

et al. 1998

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The coupling between the heme and the surrounding protein in horseradish peroxidase was studied after substituting the iron protoheme by mesoporphyrin IX to produce a sample measurable by high-resolution fluorescence spectroscopy. The inner ring phototautomerization of mesoporphyrin was used to create a variety of prosthetic group configurations that were shown to be stable at cryogenic temperatures. Due to the properties of the heme crevice, some tautomeric states are characterized by distinct spectral bands. The original band of the tautomeric form stable at room temperature (B 1 ) and two of those produced by photobleaching (B 2 , B 3 ) could be selectively studied by two techniques, i.e., energy selected fluorescence excitation and vibronic hole burning spectroscopy. The line narrowed spectra were similar in the cases of complexes B 2 and B 3 , while both are different from that of B 1 . From these spectra, four characteristic vibronic lines were selected and further studied by spectral hole burning experiments. The unusual shapes of some spectral holes were discussed and interpreted on the basis of a new approach to the principles of energy selected spectroscopy. Vibronic relaxation times were determined and found in the range of 1-11 ps. It could be shown that in the porphyrinprotein complexes created photochemically at low temperature, some specific vibronic modes are characterized by significantly increased relaxation time values. It was thus experimentally verified that the coupling to the protein is the strongest in the lowest energy configuration stable at room temperature (B 1 ), in agreement with data of pressure tuning and of Stark effect hole burning studies on the same complexes.

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A spectral hole‐burning study of long‐wavelength chlorophyll a forms in greening leaves at 5 K

Cited by 12 publications

References 20 publications

Kinetic model of primary energy transfer and trapping in photosynthetic membranes

Kinetic model of primary energy transfer and trapping in photosynthetic membranes

Fluorescence line narrowing: a high-resolution window on DNA and protein damage from chemical carcinogens

Variety in the Coupling of Mesoporphyrin IX to Apohorseradish Peroxidase C Studied by Energy Selected Fluorescence Excitation and Vibronic Hole Burning Spectroscopy

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