A specific role of iron in promoting meristematic cell division during adventitious root formation

Hilo, Alexander; Shahinnia, Fahimeh; Druege, Uwe; Franken, Philipp; Melzer, Michael; Rutten, Twan; Wirén, Nicolaus von; Hajirezaei, Mohammad‐Reza

doi:10.1093/jxb/erx248

Cited by 41 publications

(36 citation statements)

References 48 publications

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“…ARR3 and ARR4 are type-A of response regulators ( Hwang et al, 2012 ) that are quickly induced by CKs and that redundantly regulate branching patters in the shoot in response to nitrate availability ( Müller et al, 2015 ). Recently, ammonium has been shown to improve adventitious rooting in leafy cuttings of Petunia hybrida ( Hilo et al, 2017 ). Further investigations will clarify the role of DcARR3 and DcARR4 in nutrient-induced AR formation in carnation stem cuttings.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Conjugation of Auxin by GH3 Enzymes Leads to Poor Adventitious Rooting in Carnation Stem Cuttings

et al. 2018

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Commercial carnation (Dianthus caryophyllus) cultivars are vegetatively propagated from axillary stem cuttings through adventitious rooting; a process which is affected by complex interactions between nutrient and hormone levels and is strongly genotype-dependent. To deepen our understanding of the regulatory events controlling this process, we performed a comparative study of adventitious root (AR) formation in two carnation cultivars with contrasting rooting performance, “2101–02 MFR” and “2003 R 8”, as well as in the reference cultivar “Master”. We provided molecular evidence that localized auxin response in the stem cutting base was required for efficient adventitious rooting in this species, which was dynamically established by polar auxin transport from the leaves. In turn, the bad-rooting behavior of the “2003 R 8” cultivar was correlated with enhanced synthesis of indole-3-acetic acid conjugated to aspartic acid by GH3 proteins in the stem cutting base. Treatment of stem cuttings with a competitive inhibitor of GH3 enzyme activity significantly improved rooting of “2003 R 8”. Our results allowed us to propose a working model where endogenous auxin homeostasis regulated by GH3 proteins accounts for the cultivar dependency of AR formation in carnation stem cuttings.

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Section: Discussionmentioning

confidence: 99%

Enhanced Conjugation of Auxin by GH3 Enzymes Leads to Poor Adventitious Rooting in Carnation Stem Cuttings

et al. 2018

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“…Our results suggest that nuclei may be a reservoir of iron during seed development or that iron may have unknown functions in nuclei of embryos in torpedo stage. Very recently, a specific role of iron in promoting meristematic cell division in adventitious root formation has been proposed ( Hilo et al, 2017 ), however, the molecular role of iron in nuclei remains unknown.…”

Section: Discussionmentioning

confidence: 99%

Dynamic Subcellular Localization of Iron during Embryo Development in Brassicaceae Seeds

et al. 2017

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Iron is an essential micronutrient for plants. Little is know about how iron is loaded in embryo during seed development. In this article we used Perls/DAB staining in order to reveal iron localization at the cellular and subcellular levels in different Brassicaceae seed species. In dry seeds of Brassica napus, Nasturtium officinale, Lepidium sativum, Camelina sativa, and Brassica oleracea iron localizes in vacuoles of cells surrounding provasculature in cotyledons and hypocotyl. Using B. napus and N. officinale as model plants we determined where iron localizes during seed development. Our results indicate that iron is not detectable by Perls/DAB staining in heart stage embryo cells. Interestingly, at torpedo development stage iron localizes in nuclei of different cells type, including integument, free cell endosperm and almost all embryo cells. Later, iron is detected in cytoplasmic structures in different embryo cell types. Our results indicate that iron accumulates in nuclei in specific stages of embryo maturation before to be localized in vacuoles of cells surrounding provasculature in mature seeds.

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“…Soluble sugars and amino acids were determined according to Hajirezaei et al (2000) and Hilo et al (2017). Extraction and analysis of Trp, tryptamine, and serotonin was performed as described previously (Cao et al, 2006;Kang et al, 2009).…”

Section: Physiological Analysesmentioning

confidence: 99%

An Age-Dependent Sequence of Physiological Processes Defines Developmental Root Senescence

et al. 2019

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Aging-related processes in plant tissues are associated with changes in developmental and physiological processes relevant for stress tolerance and plant performance. While senescence-regulated processes have been extensively characterized in leaves, they remain poorly described in roots. Here, we investigated the physiological processes and molecular determinants underlying the senescence of seminal roots in hydroponically grown barley (Hordeum vulgare). Transcriptome profiling in apical and basal root tissues revealed that several NAC-, WRKY-, and APETALA2 (AP2)-type transcription factors were upregulated just before the arrest of root elongation, when root cortical cell lysis and nitrate uptake, as well as cytokinin concentrations ceased. At this time point, root abscisic acid levels peaked, suggesting that abscisic acid is involved in root aging-related processes characterized by expression changes of genes involved in oxidative stress responses. This temporal sequence of aging-related processes in roots is highly reminiscent of typical organ senescence, with the exception of evidence for the retranslocation of nutrients from roots. Supported by the identification of senescence-related transcription factors, some of which are not expressed in leaves, our study indicates that roots undergo an intrinsic genetically determined senescence program, predominantly influenced by plant age.

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A specific role of iron in promoting meristematic cell division during adventitious root formation

Abstract: The essential role of iron in formation of adventitious roots is associated with an enhanced allocation of this element to actively dividing cells, highlighting a specific function in meristem development.

Cited by 41 publications

References 48 publications

Enhanced Conjugation of Auxin by GH3 Enzymes Leads to Poor Adventitious Rooting in Carnation Stem Cuttings

Enhanced Conjugation of Auxin by GH3 Enzymes Leads to Poor Adventitious Rooting in Carnation Stem Cuttings

Dynamic Subcellular Localization of Iron during Embryo Development in Brassicaceae Seeds

An Age-Dependent Sequence of Physiological Processes Defines Developmental Root Senescence

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