A Specific Radioimmunoassay for the Growth Hormone (GH)-Dependent Somatomedin-Binding Protein: Its Use for Diagnosis of GH Deficiency

Blum, Werner; Ranke, Michael B.; Kietzmann, K.; Gauggel, Elisabeth; Zeisel, Hans; Bierich, J. R.

doi:10.1210/jcem-70-5-1292

Cited by 463 publications

(249 citation statements)

References 36 publications

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“…Details regarding determination of IGF-I have been presented previously (Juul et al, 1994). IGFBP-3 was determined by a radio immunoassay as previously described (Blum et al, 1990). Reagents for the assay were obtained from Mediagnost GmbH (Tübingen, Germany).…”

Section: Methodsmentioning

confidence: 99%

High intakes of skimmed milk, but not meat, increase serum IGF-I and IGFBP-3 in eight-year-old boys

Hoppe¹,

Mølgaard²,

et al. 2004

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Objective: To examine whether a high protein intake (PI) from either milk or meat, at a level often seen in late infancy, could increase s-IGF-I and s-IGF-I/s-IGFBP-3 in healthy, prepubertal children. IGF-I levels are positively associated with growth velocity in children and some studies suggest that a high animal PI can stimulate growth. During protein deprivation IGF-I decrease, but it is unknown whether a high PI can increase s-IGF-I in well-nourished children. Design: In all, 24 8-y-old boys were asked to take either 1.5 l of skimmed milk (n ¼ 12) or the same amount of protein as 250 g low fat meat (n ¼ 12) daily for 7 days. The remaining diet they could choose freely. At baseline and after 7 days, anthropometrical variables were measured, diet was registered (3-day weighed records), and s-IGF-I and s-IGFBP-3 (RIA) were determined after fast. Results: PI increased by 61% in the milk group to 4.0 g/kg/day (Po0.0001) and by 54% in the meat group to 3.8 g/kg/day (P ¼ 0.001). The high milk intake increased s-IGF-I by 19% (P ¼ 0.001) and s-IGF-I/s-IGFBP-3 by 13% (Po0.0001). There were no increases in the meat group. Conclusions: High intake of milk and not meat, increased concentrations of s-IGF-I and s-IGF-I/s-IGFBP-3 significantly. Compounds in milk and not a high PI as such seem to stimulate IGF-I. This might explain the positive effect of milk intake on growth seen in some studies.

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Section: Methodsmentioning

confidence: 99%

High intakes of skimmed milk, but not meat, increase serum IGF-I and IGFBP-3 in eight-year-old boys

Hoppe¹,

Mølgaard²,

et al. 2004

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“…The following parameters were determined in serum samples: alkaline phosphatase with a photometric method (optimierte Standard-Methode; Boehringer (17); IGFBP-3 was measured with a specific radioimmunoassay as described previously (18).…”

Section: Methodsmentioning

confidence: 99%

A new and accurate prediction model for growth response to growth hormone treatment in children with growth hormone deficiency

Schönaü

Westermann²,

Rauch³

et al. 2001

European Journal of Endocrinology

101

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Objective: To identify parameters which predict individual growth response to recombinant human GH (rhGH) therapy and to combine these parameters in a prediction model. Design: Fifty-eight prepubertal patients with GH deficiency (17 females) participated in this prospective multicenter trial with 1 year of follow-up. Methods: Auxological measurements, parameters of GH status and markers of bone metabolism were measured at baseline and at 1, 3 and 6 months after the start of rhGH treatment. Correlations with height velocity during the first 12 months of treatment HV 12 were calculated. Prediction models were derived by multiple regression analysis. Results: The model which best predicted HV 12 combined the following parameters: pretreatment bone age retardation as a fraction of chronological age, pretreatment serum levels of IGF-I, urinary levels of deoxypyridinoline (a marker of bone resorption) after 1 month of treatment and height velocity after 3 months of treatment. This model explained 89% of the variation in HV 12 with a standard deviation of the residuals of 0.93 cm/year. Defining successful rhGH therapy as a doubling of pretreatment height velocity, the model had a specificity of 90% and a sensitivity of 100% in predicting therapeutic success. Conclusions: This model is an accurate and practicable tool to predict growth response in GH-deficient children. It may help to optimize rhGH therapy by individual dose adjustment and contribute to improved overall outcomes.European Journal of Endocrinology 144 13±20

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“…Serum IGFBP-3 was analyzed by a RIA described previously (16). All serum samples were stored at K20 8C until analysis.…”

Section: Hormone Measurementsmentioning

confidence: 99%

Pharmacokinetics and pharmacodynamics of GH: dependence on route and dosage of administration

Keller¹,

Kratzsch

et al. 2007

eur j endocrinol

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Objective: Pharmacokinetic and pharmacodynamic data after recombinant human GH (rhGH) administration in adults are scarce, but necessary to optimize replacement therapy and to detect doping. We examined pharmacokinetics, pharmacodynamics, and 20 kDa GH after injection of rhGH at different doses and routes of administration. Design: Open-label crossover study with single boluses of rhGH. Methods: Healthy trained subjects (10 males, 10 females) received bolus injections of rhGH on three occasions: 0.033 mg/kg s.c., 0.083 mg/kg s.c., and 0.033 mg/kg i.m. Concentrations of 22 and 20 kDa GH, IGF-I, and IGF-binding proteins (IGFBP)-3 were measured repeatedly before and up to 36 h after injection. Results: Serum GH maximal concentration (C max ) and area under the time-concentration curve (AUC) were higher after i.m. than s.c. administration of 0.033 mg/kg (C max 35.5 and 12.0 mg/l; AUC 196.2 and 123.8). C max and AUC were higher in males than in females (P!0.01) and pharmacodynamic changes were more pronounced. IGFBP-3 concentrations showed no dose dependency. In response to rhGH administration, 20 kDa GH decreased in females and remained suppressed for 14-18 h (low dose) and 30 h (high dose). In males, 20 kDa GH was undetectable at baseline and throughout the study. Conclusions: After rhGH administration, pharmacokinetic parameters are mainly influenced by route of administration, whereas pharmacodynamic variables and 20 kDa GH concentrations are determined mainly by gender. These differences need to be considered for therapeutic use and for detection of rhGH doping.European Journal of Endocrinology 156 647-653

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A Specific Radioimmunoassay for the Growth Hormone (GH)-Dependent Somatomedin-Binding Protein: Its Use for Diagnosis of GH Deficiency

Cited by 463 publications

References 36 publications

High intakes of skimmed milk, but not meat, increase serum IGF-I and IGFBP-3 in eight-year-old boys

High intakes of skimmed milk, but not meat, increase serum IGF-I and IGFBP-3 in eight-year-old boys

A new and accurate prediction model for growth response to growth hormone treatment in children with growth hormone deficiency

Pharmacokinetics and pharmacodynamics of GH: dependence on route and dosage of administration

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