A specific method for the determination of soluble sugars in plant extracts using enzymatic analysis and its application to the sugar content of developing pear fruit buds

Blunden, Christine A.; Wilson, Michael F.

doi:10.1016/0003-2697(85)90195-2

Cited by 19 publications

(10 citation statements)

References 12 publications

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“…The monosaccharides’ content was estimated spectrophotometrically (Hitachi U-5100 UV-Vis spectrophotometer; Hitachi High-Tech Science Corporation, Tokyo, Japan) according to the Somogyi-Nelson method [ 70 , 71 ]. A sample of W. arrhiza (0.5 g) was extracted in 5 mL of 62.5% ( v / v ) MeOH in a water bath for 30 min at 60 °C [ 72 ]. The standard sample was glucose, which was prepared by the dissolving of 30 mg glucose with 62.5% ( v / v ) MeOH.…”

Section: Methodsmentioning

confidence: 99%

Brassinolide Enhances the Level of Brassinosteroids, Protein, Pigments, and Monosaccharides in Wolffia arrhiza Treated with Brassinazole

Chmur

Bajguz

2021

Plants

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Brassinolide (BL) represents brassinosteroids (BRs)—a group of phytohormones that are essential for plant growth and development. Brassinazole (Brz) is as a synthetic inhibitor of BRs’ biosynthesis. In the present study, the responses of Wolffia arrhiza to the treatment with BL, Brz, and the combination of BL with Brz were analyzed. The analysis of BRs and Brz was performed using LC-MS/MS. The photosynthetic pigments (chlorophylls, carotenes, and xanthophylls) levels were determined using HPLC, but protein and monosaccharides level using spectrophotometric methods. The obtained results indicated that BL and Brz influence W. arrhiza cultures in a concentration-dependent manner. The most stimulatory effects on the growth, level of BRs (BL, 24-epibrassinolide, 28-homobrassinolide, 28-norbrassinolide, catasterone, castasterone, 24-epicastasterone, typhasterol, and 6-deoxytyphasterol), and the content of pigments, protein, and monosaccharides, were observed in plants treated with 0.1 µM BL. Whereas the application of 1 µM and 10 µM Brz caused a significant decrease in duckweed weight and level of targeted compounds. Application of BL caused the mitigation of the Brz inhibitory effect and enhanced the BR level in duckweed treated with Brz. The level of BRs was reported for the first time in duckweed treated with BL and/or Brz.

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Section: Methodsmentioning

confidence: 99%

Brassinolide Enhances the Level of Brassinosteroids, Protein, Pigments, and Monosaccharides in Wolffia arrhiza Treated with Brassinazole

Chmur

Bajguz

2021

Plants

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“…(). Soluble sugars were measured according to Blunden & Wilson (). Starch concentration was determined following Rasmussen & Henry () by enzymatic procedure.…”

Section: Methodsmentioning

confidence: 99%

The combined effect of salinity and heat reveals a specific physiological, biochemical and molecular response in tomato plants

Rivero

Mestre

Mittler

et al. 2013

Plant Cell & Environment

334

293

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Many studies have described the response mechanisms of plants to salinity and heat applied individually; however, under field conditions some abiotic stresses often occur simultaneously. Recent studies revealed that the response of plants to a combination of two different stresses is specific and cannot be deduced from the stresses applied individually. Here, we report on the response of tomato plants to a combination of heat and salt stress. Interestingly, and in contrast to the expected negative effect of the stress combination on plant growth, our results show that the combination of heat and salinity provides a significant level of protection to tomato plants from the effects of salinity. We observed a specific response of plants to the stress combination that included accumulation of glycine betaine and trehalose. The accumulation of these compounds under the stress combination was linked to the maintenance of a high K + concentration and thus a lower Na + /K + ratio, with a better performance of the cell water status and photosynthesis as compared with salinity alone. Our findings unravel new and unexpected aspects of the response of plants to stress combination and provide a proposed list of enzymatic targets for improving crop tolerance to the abiotic field environment.

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“…Samples in 75% methanol were treated with 10- to 15-mg activated charcoal (Sigma C-5385) per mL to remove substances that might interfere with enzymatic analyses [21,37]. The slurry was stirred, and then allowed to settle overnight at -20°C.…”

Section: Methodsmentioning

confidence: 99%

Compartmentation of sucrose during radial transfer in mature sorghum culm

Tarpley¹,

Vietor

2007

BMC Plant Biol

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Background: The sucrose that accumulates in the culm of sorghum (Sorghum bicolor (L.) Moench) and other large tropical andropogonoid grasses can be of commercial value, and can buffer assimilate supply during development. Previous study conducted with intact plants showed that sucrose can be radially transferred to the intracellular compartment of mature ripening sorghum internode without being hydrolysed. In this study, culm-infused radiolabelled sucrose was traced between cellular compartments and among related metabolites to determine if the compartmental path of sucrose during radial transfer in culm tissue was symplasmic or included an apoplasmic step. This transfer path was evaluated for elongating and ripening culm tissue of intact plants of two semidwarf grain sorghums. The metabolic path in elongating internode tissue was also evaluated.

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A specific method for the determination of soluble sugars in plant extracts using enzymatic analysis and its application to the sugar content of developing pear fruit buds

Cited by 19 publications

References 12 publications

Brassinolide Enhances the Level of Brassinosteroids, Protein, Pigments, and Monosaccharides in Wolffia arrhiza Treated with Brassinazole

Brassinolide Enhances the Level of Brassinosteroids, Protein, Pigments, and Monosaccharides in Wolffia arrhiza Treated with Brassinazole

The combined effect of salinity and heat reveals a specific physiological, biochemical and molecular response in tomato plants

Compartmentation of sucrose during radial transfer in mature sorghum culm

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