A specific method for the analysis of hydroxyproline in tissues and urine

Prockop, Darwin J.; Udenfriend, Sidney

doi:10.1016/0003-2697(60)90050-6

Cited by 1,040 publications

(276 citation statements)

References 7 publications

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“…Hydroxyproline was measured in samples of approximately 10 mg of tissue (dry weight) from the free wall of the left ventricle according to the method of Prockop and Udenfriend. 25 In addition, saline-soluble collagen was measured from pooled left ventricles according to the method of Nimni. 26 For this measurement, four or five left ventricles were homogenized at 4°C in 20 volumes of 0.5 m NaCl in a Virtis homogenizer at half the maximum speed setting for 5 minutes.…”

Section: Methodsmentioning

confidence: 99%

The effect of lathyrogen beta-amino proprionitrile (BAPN) on the mechanical properties of experimentally hypertrophied rat cardiac muscle.

Bing¹,

Fanburg²,

Brooks³

et al. 1978

Circulation Research

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SUMMARY yS-Amino proprionitrile (BAPN) at dietary concentrations of 1, 5, and 10 g BAPN/kg rat chow was administered to rats for 14-21 days following surgical constriction of the ascending aorta. Five and 10 g BAPN/kg rat chow prevented the increase in left ventricular collagen content which occurred with cardiac hypertrophy in rats following aortic constriction. In spite of this block in the increase in collagen in the ventricles, isolated trabecular muscles from hypertrophied hearts showed a decrease in maximum velocity of shortening at a preload of 0.5 g/mm 2 (max V) and an increase in time to peak tension as compared with values for sham-operated animals. Max V for rats with aortic constriction was decreased 0.57 muscle length/sec as compared with sham-operated animals (P< 0.01) whereas time to peak tension was prolonged by 12 msec (P < 0.05). In rats with aortic constriction receiving 10 g BAPN/kg rat chow, max V was decreased 0.66 muscle length/sec (P < 0.05), and time to peak tension was prolonged by 21 msec (P < 0.001). Resting tension was increased to 1.70 ± 0.18 (mean ± SEM) g/mm 2 as compared with shams (1.22 ± 0.10 g/mm 2 ; P < 0.002) in cardiac hypertrophy without BAPN. However, the increase in resting tension was not seen when animals with aortic constriction received 10 g BAPN/kg rat chow [1.23 ± 0.9 g/mm 2 as compared with shams, 1.15 ± 0.09 g/mm 2 (not significant)]. We conclude that the decrease in maximum velocity of shortening and prolongation of time to peak tension in experimental cardiac hypertrophy occur independently of elevated collagen content, whereas elevations in resting tension appear to depend upon an increase in collagen content of these hearts.IN THE past decade, numerous studies have attempted to characterize the mechanical performance of hypertrophied cardiac muscle. Several investigators 1 " 6 have reported a decrease in maximum shortening velocity of hypertrophied cardiac muscle when the hypertrophy was induced by a pressure overload. Whether the decrease in shortening velocity reflects a depression of cardiac "contractility" is a matter of controversy. 7 " 9 It seems clear, nonetheless, that an abnormality of cardiac performance follows acute experimental pressure overload. Additional mechanical changes have been described to accompany cardiac hypertrophy induced by pressure overload. These include a prolongation of contraction 2 ' 4l 6 and a change in passive compliance. 2 ' 5 In addition to the increase in RNA 10 ' n and protein synthesis, 12 ' 13 which accompanies experimental cardiac hypertrophy, enhanced thymidine incorportion into DNA has been demonstrated primarily in fibroblasts. 11 ' 14 ' lb Also, increased quantities of collagen are found in hypertrophied heart muscle. 2 ' 16 " 18 /?-amino proprionitrile (BAPN) is a lathyrogen which has been shown in vivo and in tissue culture to inhibit the cross-linking of collagen 19 ' 20 and elastin 21 probably by blocking the initial step in the enzymatic conversion of lysyl residues in peptide linkages to their aldehyde de...

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Section: Methodsmentioning

confidence: 99%

The effect of lathyrogen beta-amino proprionitrile (BAPN) on the mechanical properties of experimentally hypertrophied rat cardiac muscle.

Bing¹,

Fanburg²,

Brooks³

et al. 1978

Circulation Research

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show abstract

“…The samples were pulverised in liquid nitrogen, lyophilised, weighed and kept at -30°C. In each rat, both the control segments removed at operation and the samples containing the anastomosis, were analsyed for hydroxyproline as described elsewhere (Hesp et al, 1984), essentially according to Prockop and Udenfriend (1960 …”

Section: Cytostatic Agentsmentioning

confidence: 99%

Intraperitoneal cytostatics impair healing of experimental intestinal anastomoses

Zuidewijn

Hendriks²,

Wobbes³

et al. 1991

Br J Cancer

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Summary We investigated the effect of two doses of cytostatics, administered intraperitoneally during 5 consecutive days, on the healing of ileal and colonic anastomoses constructed on the third day. The cytostatics regimen consisted of a combination of 5-fluorouracil, bleomycin and cisplatin at 10, 2 and 0.35 mg kg-'d', respectively, or at twice higher doses. The lower dose was similar to that given intravenously in previous experiments. Rats were sacrificed 3 or 7 days after operation. No effects of cytostatics were observed after 3 days, neither on anastomotic bursting pressure nor on hydroxyproline concentration (jLg/mg dry weight) or content (ug cm-'). Profound effects were seen at 7 days. In the high dose group, bursting pressures in both anastomoses were greatly reduced with respect to the control group. Concurrently, collagen synthesis was severely impaired, as indicated by sustained decreased hydroxyproline concentrations and content. The lower dose of cytostatics showed essentially similar effects on hydroxyproline parameters, but affected anastomotic strength less dramatically. The data indicate that, while intraperitoneal chemotherapy may show less detrimental systemic toxicity and thus allow higher doses, its application as an adjunct to gastrointestinal surgery may be limited because of its severe effects on anastomotic repair.

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“…Quantification of myocardial hydroxyproline (HOP) concentrations, an indicator of collagen content, was performed as previously described 28. Briefly, the tissue was minced, its mass determined, and hydrolyzed overnight in 2 mL of 6 M hydrochloric acid at 110°C.…”

Section: Methodsmentioning

confidence: 99%

The neuroprotective agent Rasagiline mesylate attenuates cardiac remodeling after experimental myocardial infarction

Varela¹,

Mavroidis

Katsimpoulas³

et al. 2017

ESC Heart Failure

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AimRasagiline mesylate (N‐propargyl‐1 (R)‐aminoindan) (RG) is a selective, potent irreversible inhibitor of monoamine oxidase‐B with cardioprotective and anti‐apoptotic properties. We investigated whether it could be cardioprotective in a rat model undergoing experimental myocardial infarction (MI) by permanent ligation of the left anterior descending coronary artery.Methods and resultsRG was administered, intraperitoneally, for 28 days (2 mg/kg) starting 24 h after MI induction. Echocardiography analysis revealed a significant reduction in left ventricular end‐systolic and diastolic dimensions and preserved fractional shortening in RG‐treated compared with normal saline group at 28 days post‐MI (31.6 ± 2.3 vs. 19.6 ± 1.8, P < 0.0001), respectively. Treatment with RG prevented tissue fibrosis as indicated by interstitial collagen estimation by immunofluorescence staining and hydroxyproline content and attenuated the number of apoptotic myocytes in the border zone (65%) as indicated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Caspase 3 relative protein levels were significantly decreased in the non‐infarcted myocardium. Markedly decreased malondialdehyde levels in the border zone indicate a reduction in tissue oxidative stress.ConclusionsOur study demonstrates a positive effect of RG in the post‐MI period with a significant attenuation in cardiac remodelling.

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A specific method for the analysis of hydroxyproline in tissues and urine

Cited by 1,040 publications

References 7 publications

The effect of lathyrogen beta-amino proprionitrile (BAPN) on the mechanical properties of experimentally hypertrophied rat cardiac muscle.

The effect of lathyrogen beta-amino proprionitrile (BAPN) on the mechanical properties of experimentally hypertrophied rat cardiac muscle.

Intraperitoneal cytostatics impair healing of experimental intestinal anastomoses

The neuroprotective agent Rasagiline mesylate attenuates cardiac remodeling after experimental myocardial infarction

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