A specific fungal transcription factor controls effector gene expression and orchestrates the establishment of the necrotrophic pathogen lifestyle on wheat

Jones, Darcy A. B.; John, Evan; Rybak, Kasia; Phan, H.; Singh, Karam B.; Lin, Shao-Yu; Solomon, Peter S.; Oliver, Richard P.; Tan, Kar‐Chun

doi:10.1038/s41598-019-52444-7

Cited by 39 publications

(65 citation statements)

References 80 publications

(112 reference statements)

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“…For example, a well conserved Zn2Cy6 transcription factor (PnPf2) is involved in the expression of genes associated with nutrient assimilation and numerous effectors in the phyto-necrotrophic fungus Parastagonospora nodorum . Disrupting the transcription factor expression dramatically alters the plant cell wall degradation, asexual reproduction, and virulence [ 84 ].…”

Section: Discussionmentioning

confidence: 99%

Genome Expression Dynamics Reveal the Parasitism Regulatory Landscape of the Root-Knot Nematode Meloidogyne incognita and a Promoter Motif Associated with Effector Genes

Rocha

Bournaud

Dazenière

et al. 2021

Genes

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Root-knot nematodes (genus Meloidogyne) are the major contributor to crop losses caused by nematodes. These nematodes secrete effector proteins into the plant, derived from two sets of pharyngeal gland cells, to manipulate host physiology and immunity. Successful completion of the life cycle, involving successive molts from egg to adult, covers morphologically and functionally distinct stages and will require precise control of gene expression, including effector genes. The details of how root-knot nematodes regulate transcription remain sparse. Here, we report a life stage-specific transcriptome of Meloidogyne incognita. Combined with an available annotated genome, we explore the spatio-temporal regulation of gene expression. We reveal gene expression clusters and predicted functions that accompany the major developmental transitions. Focusing on effectors, we identify a putative cis-regulatory motif associated with expression in the dorsal glands, providing an insight into effector regulation. We combine the presence of this motif with several other criteria to predict a novel set of putative dorsal gland effectors. Finally, we show this motif, and thereby its utility, is broadly conserved across the Meloidogyne genus, and we name it Mel-DOG. Taken together, we provide the first genome-wide analysis of spatio-temporal gene expression in a root-knot nematode and identify a new set of candidate effector genes that will guide future functional analyses.

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Section: Discussionmentioning

confidence: 99%

Genome Expression Dynamics Reveal the Parasitism Regulatory Landscape of the Root-Knot Nematode Meloidogyne incognita and a Promoter Motif Associated with Effector Genes

Rocha

Bournaud

Dazenière

et al. 2021

Genes

View full text Add to dashboard Cite

show abstract

“…For example, a well conserved Zn2Cy6 transcription factor (PnPf2) is involved in the expression of genes associated with nutrient assimilation and numerous effectors in the phyto-necrotrophic fungus Parastagonospora nodorum. Disrupting the transcription factor expression dramatically alters the plant cell wall degradation, axesual reproduction as well as virulence [84].…”

Section: Discussionmentioning

confidence: 99%

Genome expression dynamics reveals parasitism regulatory landscape of the root-knot nematode Meloidogyne incognita and a promoter motif associated with effector genes

Rocha

Bournaud

Dazenière

et al. 2021

Preprint

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Root-knot nematodes are the major contributor to the crop losses caused by nematodes. Root-knot nematodes secrete effectors into the plant, derived from two sets of pharyngeal gland cells, to manipulate host physiology and immunity. Successful completion of the life cycle, involving successive molts from egg to adult, covers morphologically and functionally distinct stages and will require precise control of gene expression, including effectors. The details of how root-knot nematodes regulate transcription remain sparse. Here, we report a life stage-specific transcriptome of Meloidogyne incognita. Combined with an available annotated genome, we explore the spatio-temporal regulation of gene expression. We reveal gene expression clusters and predicted functions that accompany the major developmental transitions. Focusing on effectors, we identify a putative cis-regulatory motif associated with expression in the dorsal glands: providing an insight into effector regulation. We combine the presence of this motif with several other criteria to predict a novel set of putative dorsal gland effectors. Finally, we show this motif, and thereby its utility, is broadly conserved across the Meloidogyne genus and termed it Mel-DOG. Taken together, we provide the first analysis of spatio-temporal gene expression in a root-knot nematode, and identify a new set of candidate effector genes that will feed future functional analyses.

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“…Con7 (Lin et al, 2018), Pf2 (Jones et al, 2019), BcYOH1 (Simon et al, 2013), Rua1 (Teichmann et al, 2010), BcabaR1 (Wang, Hu, et al, 2018) Note: Detailed reviews on these and other methods not yet widely used in plant-pathogenic fungi can be found (Levati et al, 2016;Orenstein & Shamir, 2017;Slattery et al, 2014;Viola & Gonzalez, 2016).…”

Section: Molecular Methods Description Examplesmentioning

confidence: 99%

Transcription factor control of virulence in phytopathogenic fungi

John

Singh

Oliver

et al. 2021

Molecular Plant Pathology

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The symptoms of plant diseases such as rusts, blasts, smuts, blotches, blights, and mildews, along with various prescriptions for their mitigation, have been recorded since antiquity (Dark & Gent, 2001;Dugan, 2008;Wu et al., 2019). Until the establishment of the germ theory of disease in the 19th century, the nature of the causal agents remained obscure (Kelman & Peterson, 2002). Subsequently, numerous plant-pathogenic microorganisms have been identified (Crous et al.

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A specific fungal transcription factor controls effector gene expression and orchestrates the establishment of the necrotrophic pathogen lifestyle on wheat

Cited by 39 publications

References 80 publications

Genome Expression Dynamics Reveal the Parasitism Regulatory Landscape of the Root-Knot Nematode Meloidogyne incognita and a Promoter Motif Associated with Effector Genes

Genome Expression Dynamics Reveal the Parasitism Regulatory Landscape of the Root-Knot Nematode Meloidogyne incognita and a Promoter Motif Associated with Effector Genes

Genome expression dynamics reveals parasitism regulatory landscape of the root-knot nematode Meloidogyne incognita and a promoter motif associated with effector genes

Transcription factor control of virulence in phytopathogenic fungi

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