A specific assay for subnanogram concentrations of reserpine in human plasma

Tripp, Spencer L.; Williams, Elinor; Wagner, William E.; Lukas, George

doi:10.1016/0024-3205(75)90200-3

Cited by 18 publications

(1 citation statement)

References 14 publications

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“…Satisfactory agreement exists between the absolute values obtained with our newer procedure and those obtained from previous assays performed in our laboratory and based on the use of radio labelled reserpine. The solutions adopted by us for detecting and identifying small amounts of reserpine in animal tissues are believed to represent also an interesting alternative to radioisotopic methods and to a recently published fluorimetric procedure (22), for the determination of this drug in human plasma. Table I Reserpine levels in the rat brain: comparison of mass fragmentographic and radioisotopic determinations.…”

Section: -Discussionmentioning

confidence: 99%

Persistence of reserpine in rat brain: gas chromatographic — mass fragmentographic identification and quantitative determination

Pantarotto

Belvedere

Frigerio

et al. 1976

European Journal of Drug Metabolism and Pharmacokinetics

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The highly sensitive and specific combination of a gas chromatographic procedure with mass fragmentographic detection was adopted in order to determine the nanogram concentration of reserpine-like material persisting in rat brain, with more adequate information as to its actual chemical structure.Quantification was achieved by focussing the mass spectrometer upon the ions at mle 608, the molecular ion and base peak in the spectrum of reserpine; mle 634, the molecular ion of rescinnamine (internal standard) and mle 195, a fragment ion common to both reserpine and deserpidine (the latter also used as an internal standard for multiple ion detection).No column adsorption, decomposition or C 3-epimerization was observed during the analyses when trideuterated reserpine was used as a carrier. Satisfactory agreement resulted from the comparison of the data of radioisotopic assays previously performed in our laboratory, with those obtained by the use of the present method.

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Section: -Discussionmentioning

confidence: 99%

Persistence of reserpine in rat brain: gas chromatographic — mass fragmentographic identification and quantitative determination

Pantarotto

Belvedere

Frigerio

et al. 1976

European Journal of Drug Metabolism and Pharmacokinetics

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Synthesis of specifically ²H‐labeled reserpines, 3,4,5‐trimethoxybenzoic acids, and syringic acid

Roth

Fischer

Pachta

et al. 1982

Labelled Comp Radiopharmac

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SUMMARY3,4,5-Trimethoxy-'Hg-, 4-metho~y-3,5-dimethoxy-~H~-, and 4-hydroxy-3,5-dimethoxy-*H6-benzoic acids were prepared from g-propyl 3,4,5-trihydroxybenzoate (n-propyl gallate) by means of appropriate alkylationhydrolysis sequences employing iodor~iethane-~H~ or dimethyl-'H6-sulfate as the deuterium source. similarly prepared from ethyl 4-hydroxy-3,5-dirnethoxybenzoate.labeled trimethoxybenzoic acids were converted to the corresponding 2H-labeled reserpines by condensation of the acid chlorides with methyl reserpate in pyridine according to the classical procedure. The labeled reserpine analog methyl 18-0-(4-hydroxy-3,5-dimethoxy-2H6)benzoyl reserpate was likewise prepared from 4-hydro~y-3,5-dimethoxy-~H~-benzoic acid via the intermediate methyl 18-0-(4-ethoxycarbonyloxy-3,5-dimethoxy-*H~-benzoy1)reserpate (syrosing~pine-~H~). The isotopic purity of each compound exceeded 99 atom percent ZH.

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Antibody Specificity studies for Reserpine, its Metabolites, and Synthetic Reserpine Congeners: Radioimmimoassay

Loeffler

Schran

1979

Journal of Pharmaceutical Sciences

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A specific assay for subnanogram concentrations of reserpine in human plasma

Cited by 18 publications

References 14 publications

Persistence of reserpine in rat brain: gas chromatographic — mass fragmentographic identification and quantitative determination

Persistence of reserpine in rat brain: gas chromatographic — mass fragmentographic identification and quantitative determination

Synthesis of specifically ²H‐labeled reserpines, 3,4,5‐trimethoxybenzoic acids, and syringic acid

Antibody Specificity studies for Reserpine, its Metabolites, and Synthetic Reserpine Congeners: Radioimmimoassay

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A specific assay for subnanogram concentrations of reserpine in human plasma

Cited by 18 publications

References 14 publications

Persistence of reserpine in rat brain: gas chromatographic — mass fragmentographic identification and quantitative determination

Persistence of reserpine in rat brain: gas chromatographic — mass fragmentographic identification and quantitative determination

Synthesis of specifically 2H‐labeled reserpines, 3,4,5‐trimethoxybenzoic acids, and syringic acid

Antibody Specificity studies for Reserpine, its Metabolites, and Synthetic Reserpine Congeners: Radioimmimoassay

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Synthesis of specifically ²H‐labeled reserpines, 3,4,5‐trimethoxybenzoic acids, and syringic acid