A Specific Absorbance to Estimate a Protein by Lowry’s Method

Ranjini, HS; Udupa, Padmanabha; Kamath, Shobha; Setty, M. Manjunath; Hadapad, Basavaraj S

doi:10.1166/asl.2017.8509

Cited by 7 publications

(6 citation statements)

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“…Samples were combined right away, pupating again after each addition. The absorbance was measured at 650 nm and blanked on the water-only control after the color had been allowed to develop for 30 minutes at room temperature (Ranjini et al, 2017).…”

Section: Estimation Of Total Protein By Lowry's Methodsmentioning

confidence: 99%

Study on rice water composition and preparation of antidandruff shampoo against Malassezia species

Balakrishnaraja,

Ranjith,

Aniskumar

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Now days, there are many problems for humans. On top of that, hair fall is a major problem noticed by teenagers. One of the main reasons for hair fall is dandruff and body heat. So, dandruff is caused by the fungus Malassezia species, and for that problem, we have a simple solution to tackle it. Rice water is a simple and effective solution for this hair fall problem. The fungal sample was isolated and identified and prepare a shampoo using rice water as a major source. Total carbohydrate and protein was determined. The prepared antifungal shampoo was tested using the well-diffusion method against the fungus Malassezia sps, which causes dandruff. The prepared rice water shampoo gives a positive result and has a high zone of inhibition against the Malassezia species. The antifungal activity was performed on the Malassezia species and shows the best result.

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Section: Estimation Of Total Protein By Lowry's Methodsmentioning

confidence: 99%

Study on rice water composition and preparation of antidandruff shampoo against Malassezia species

Balakrishnaraja,

Ranjith,

Aniskumar

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…After centrifuging at 4000 × g for 30 minutes, the supernatant was collected and freeze‐dried to obtain the powders of the water‐solubilized extract, named as DSCH. Protein content was measured by Lowry method using a protein assay kit (Beijing Dingguo Biotech Co., Ltd) …”

Section: Methodsmentioning

confidence: 99%

“…Protein content was measured by Lowry method using a protein assay kit (Beijing Dingguo Biotech Co., Ltd). 20…”

Section: Preparation Of Collagen Hydrolysates Of Deer Sinewmentioning

confidence: 99%

Transdermal permeation effect of collagen hydrolysates of deer sinew on mouse skin, ex vitro, and antioxidant activity, increased type I collagen secretion of percutaneous proteins in NIH/3T3 cells

Zhang

Pan

Dong³

et al. 2019

J of Cosmetic Dermatology

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Background:The collagen hydrolysates as a cosmetic material have already been wide application. At present, few studies concern with transdermal behavior of collagen hydrolysates in vitro.Objective: Deer sinew contains rich collagen with a content of 82.12%. Thus, this article mainly studies the transdermal effect of collagen hydrolysates of deer sinew (DSCH) on mouse skin, ex vitro, and to explore skincare protection of percutaneous proteins. Methods: Collagen hydrolysates of deer sinew were extracted by 0.2% HCl and a two-step enzymatic method of pepsin-trypsin. The content of 17 amino acids of DSCH was detected by precolumn derivatization RP-HPLC. Using Franz diffusion cell systems studied the transdermal effect of DSCH and then examined the percutaneous rate and molecular weight distribution of percutaneous proteins (PP). Further, we studied the bioactivity of PP in vitro, such as the total antioxidant capacity and collagen secretion in NIH/3T3 cells. Results: About 8.0% DSCH could penetrate skin of mouse, the molecular weight of PP mainly distributed in 5 ~ 13 kDa, accounted for 91.55%. Compared with the antioxidant activity of DSCH, PP had obvious antioxidant activity of scavenging radical cation. Meanwhile, PP promoted cell proliferation and collagen I secretion in fibroblast cells; however, level of type III collagen has no change. Conclusion: Collagen hydrolysates of deer sinew may be used as cosmetic material to protect the skin from oxidative stress, to prevent premature skin aging. K E Y W O R D S antioxidant activity, collagen hydrolysates, deer sinew, percutaneous proteins, percutaneous rate, type I collagen How to cite this article: Zhang H, Pan D, Dong Y, et al. Transdermal permeation effect of collagen hydrolysates of deer sinew on mice skin, ex vitro, and antioxidant activity, increased type I collagen secretion of percutaneous proteins in NIH/3T3 cells.

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“…Sample elution was carried out using a NaCl gradient up to 1 M [28]. Screening of esterase assay [26], phosphatase assay [27], and estimation of protein concentration [29] was carried out at each step of purification. Purified samples were used for SDS-PAGE analysis to determine the molecular weight of organophosphate degrading enzymes.…”

Section: Monocrotophos Degrading Enzyme Profilementioning

confidence: 99%

Degradation and detoxification of monocrotophos using bacterial consortium

Jokhakar¹,

Dudhagara²

2022

J App Biol biotech

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The uncontrol use of organophosphate insecticide in agricultural fields to protect crop yields imposes severe environmental issues due to its long persistence in soil and water. For cotton, maize, and sugarcane, monocrotophos is a widely used and effective organophosphate pesticide. To lessen the harm to the environment, biodegradation and detoxification of pesticides need to be developed. In the present study, the polluted site Amlakhadi, Gujarat, India, was explored for the isolation of monocrotophos degrading bacteria. Gordonia terrae, Pseudomonas aeruginosa, Serratia surfactantfaciens, and a consortium of these three bacteria were chosen for further study on degradation after being tested for their tolerance to monocrotophos. Accelerated degradation by the consortium was confirmed through spectrometry and high performance liquid chromatographic analysis. Trimethyl phosphate and acetamide, N-acetyl N-methyl was generated by the degradation of monocrotophos using consortium culture, confirmed by the gas chromatography-mass spectrometry analysis. The production of phosphotriesterase enzyme facilitated the degradation of monocrotophos and the molecular weight of phosphotriesterase was found between a range of 35-67 kDa. Degraded metabolites were found to be less toxic than undegraded monocrotophos by cytotoxicity and phytotoxicity tests. Along with monocrotophos degradation and detoxification, isolates also exhibited plant growthpromoting traits. Thus, this screening might be helpful to carry out effective in situ degradation of monocrotophos with the consortium-based formulated prototype for the rectification of polluted sites along with plant growth.

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A Specific Absorbance to Estimate a Protein by Lowry’s Method

Cited by 7 publications

References 0 publications

Study on rice water composition and preparation of antidandruff shampoo against Malassezia species

Study on rice water composition and preparation of antidandruff shampoo against Malassezia species

Transdermal permeation effect of collagen hydrolysates of deer sinew on mouse skin, ex vitro, and antioxidant activity, increased type I collagen secretion of percutaneous proteins in NIH/3T3 cells

Degradation and detoxification of monocrotophos using bacterial consortium

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