A species difference in glycerol-3-phosphate metabolism reveals metabolic adaptations in hares

Abstract

The temperate climate adapted brown hare (Lepus europaeus) and the cold-adapted mountain hare (Lepus timidus) are evolutionarily closely related and interfertile. Still, their cultured skin fibroblasts show clear differences in the expression of genes related to basic cellular metabolism. To study this further, we utilized targeted metabolomics analysis, metabolite tracing, and high-resolution respirometry, and identified significant differences in metabolic pathways associated with adaptive thermogenesis, including a higher rate of glycerol 3-phosphate (G3P) production in the mountain hare. We therefore investigated mitochondrial heat production and its dependence on G3P in the two hare species. The mountain hare maintained lower mitochondrial temperature and had weaker thermal change following OXPHOS inhibition. Manipulating mitochondrial glycerol 3-phosphate dehydrogenase (GPD2) levels demonstrated its role in mitochondrial thermogenesis and revealed species-specific function in maintaining mitochondrial membrane potential. This study unveils previously undocumented and unexpected species differences in the mitochondrial properties of fibroblasts that could indicate differences in metabolic adaptability. These findings also demonstrate the utility of cell culture models in assessing trait differences between species and their evolutionary significance, contributing to a deeper understanding of metabolic adaptation in animals and underscoring the potential of in vitro approaches for eco-physiological studies.